HMG-CoA reductase from Camphor Tulsi (Ocimum kilimandscharicum) regulated MVA dependent biosynthesis of diverse terpenoids in homologous and heterologous plant systems

Bansal, Shilpi; Narnoliya, Lokesh Kumar; Mishra, Bhawana; Chandra, Muktesh; Yadav, Radha; Sangwan, Rajender Singh

doi:10.1038/s41598-017-17153-z

Cited by 38 publications

(11 citation statements)

References 52 publications

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“…However Ocimum extract is highly aromatic. 12,18 Hence the possibility of stimulating the insect's olfactory system and repelling them is a possible mechanism by which efficacy of Ocimum extract was observed in our study. A single spray was effective in repelling the fungus gnats for just over 4 hr, as the flies either flew away or were observed deeply buried into the compost.…”

Section: Discussionsupporting

confidence: 51%

Efficacy of Natural Extract from Ocimum Species against Fungus Gnats Infesting Plant Saplings

Kumar¹,

Kumar

2020

BEMS Reports

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Background: Fungus gnats around household plants besides being annoying can also compromise the growth the plant saplings. Hence effectively repelling them without compromising their ecological purpose is necessary. Materials and Methods: We tested here the effectiveness of an aromatic natural extract of the Ocimum species in repelling the Fungus gnats naturally infesting Catharanthus roseus plant saplings. Ocimum extract was sprayed on the plant saplings and monitored for the presence of the Fungus gnats. The binding potential of selected compounds in the Ocimum extract was evaluated against known insect specific olfactory receptors to understand the mechanism of action. Results: Ocimum extract effectively repelled and reduced the fungus gnats around the plant saplings following treatment over 4 days. This improved the growth of the plant saplings. Selected compounds from Ocimum extract had physiologically effective binding affinity with the insect specific olfactory receptors. Conclusion: Ocimum extract spray is a cost effective and ecologically favourable approach for controlling fungus gnats infesting plant saplings.

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Section: Discussionsupporting

confidence: 51%

Efficacy of Natural Extract from Ocimum Species against Fungus Gnats Infesting Plant Saplings

Kumar¹,

Kumar

2020

BEMS Reports

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“…Subsequently additional steps (60 °C for 15 s, 95 °C for 15 s and 37 °C for 2 min) were followed to get the dissociation curve. Actin of O. sanctum (details provided in the Supplementary Table 1) was used as an endogeneous control to quantify the relative mRNA levels 38,87 . ∆∆C t method was used for relative quantification of gene transcripts through Sequence Detection System (SDS) software version 2.2.1.…”

Section: Methodsmentioning

confidence: 99%

Comparative temporal metabolomics studies to investigate interspecies variation in three Ocimum species

Rastogi

Shah

Kumar

et al. 2020

Sci Rep

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Ocimum is one of the most revered medicinally useful plants which have various species. each of the species is distinct in terms of metabolite composition as well as the medicinal property. Some basil types are used more often as an aromatic and flavoring ingredient. It would be informative to know relatedness among the species which though belong to the same genera while exclusively different in terms of metabolic composition and the operating pathways. in the present investigation the similar effort has been made in order to differentiate three commonly occurring Ocimum species having the high medicinal value, these are Ocimum sanctum, O. gratissimum and O. kilimandscharicum. the parameters for the comparative analysis of these three Ocimum species comprised of temporal changes in number leaf trichomes, essential oil composition, phenylpropanoid pathway genes expression and the activity of important enzymes. O. gratissimum was found to be richest in phenylpropanoid accumulation as well as their gene expression when compared to O. sanctum while O. kilimandscharicum was found to be accumulating terpenoid. in order to get an overview of this qualitative and quantitative regulation of terpenes and phenylpropenes, the expression pattern of some important transcription factors involved in secondary metabolism were also studied. Ocimum is one of the highly important medicinal plants used worldwide in the traditional medicinal systems. It is commonly known as 'basil' but is popular as 'Tulsi' in India. Not only in medicine, basil also has religious importance 1,2. Ocimum belongs to family Lamiaceae and subfamily Nepetoideae which contains strongly fragrant plants marked by the presence of essential oils comprising of sesquiterpenes, monoterpenes and phenylpropanoids. Eugenol, methylchavicol and linalool are the major compounds found in most basil species, however, the composition of every chemical constituent differ in different species or varieties 1. Another feature in the plants of Lamiaceae family is the presence of oil glands called glandular trichomes on the leaf surface. Mainly there are two types of oil glands 3 that is, peltate and capitate. The glands are differentiated by the number and size of head cells occurring on the basil leaf surface, and the phenylpropenes are exclusively synthesized in the peltate glands 4. Surplus glandular trichomes in plants and their association with essential oil biosynthesis in numerous plants including peppermint 5-14 , lima bean 15-18 , and tomato 19-21 have been talked about, which has improved our comprehension of isoprenoid production in plants. The general phenylpropanoid pathway produces a wide range of secondary metabolites originating from the shikimate pathway intermediates. However, the role and impact of this gene family need to be established 22. Phenylpropanoids contribute to both abiotic and biotic plant stress responses. Apart being the indicators of stress, they also help in acquiring resistance against pests 23. They support the incursion of fresh habitation ...

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“…Camphor Basil is unique in possessing terpenoids while other Ocimum species are popular for phenylpropanoids as major components of essential oil. 6,7 Methanol and aqueous extracts of O. kilimandscharicum inhibit HIV-1 reverse transcriptase in vitro. 8 Due to wholehearted demand in ethnomedicine in modern society, logical proof for the usefulness of numerous herbal remedies for various conditions is required and has been reported for a few of the phytomedicines.…”

Section: Issn: 2250-1177mentioning

confidence: 99%

Evaluation of preliminary phytochemical screening, acute toxicity and antioxidant profile of Ocimum kilimandscharicum

Saxena

Mittal

Siddiqui³

2019

J. Drug Delivery Ther.

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Nature has offered us diverse curative herbs having with powerful antioxidant phytochemicals. Ocimum (Lamiaceae) is a notable source of volatile oils and flavouring agents in general and primarily of eugenol, methyl eugenol, linalool, methyl chavicol, etc. Karpoora Thulasi is a member of this genus; nevertheless, not much literature has been reported on its safety and antioxidant potential. In this investigation, we did a pre-clinical safety assessment of concentrate of O. kilimandscharicum on Sprague Dawley rodents. Toxicological concordat of the O. kilimandscharicum concentrate was carried out following OECD guidelines 423. Further, to verify the traditional efficacy and elucidate the mechanism, the present study compared in-vitro antioxidant activity of the plant by DPPH, ABTS and Hydroxyl radical scavenging method using ascorbic acid as the standard. In acute oral toxicity, no treatment-related death or toxic signs were observed. Moreover, the study revealed that the O. kilimandscharicum extract could be well tolerated up to the dose 2000 mg/kg body weight and could be classified as Category 5. Moreover, ABTS free radical activity of the extract was 79.2148 while that of DDPH Inhibition potential was found to be 70.72758. Our findings present substantiation that the crude extracts of O. kilimandscharicum is a likely source of natural antioxidants, and this justified its long-established uses. Keywords: Ocimum kilimandscharicum, Acute Toxicity, In-vitro Antioxidant Activity DPPH, ABTS

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HMG-CoA reductase from Camphor Tulsi (Ocimum kilimandscharicum) regulated MVA dependent biosynthesis of diverse terpenoids in homologous and heterologous plant systems

Cited by 38 publications

References 52 publications

Efficacy of Natural Extract from Ocimum Species against Fungus Gnats Infesting Plant Saplings

Efficacy of Natural Extract from Ocimum Species against Fungus Gnats Infesting Plant Saplings

Comparative temporal metabolomics studies to investigate interspecies variation in three Ocimum species

Evaluation of preliminary phytochemical screening, acute toxicity and antioxidant profile of Ocimum kilimandscharicum

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