hMLH1 promoter methylation is an early event in oral cancer

DNA methylation analysis of cancer-related genes in oral epithelial cells of healthy smokers

“…39 This profile was shown in many cases of oral cancer at early stages and in the later stages of disease in half of the cases, suggesting that methylation of the MLH1 gene promoter is an early event and is maintained during tumour progression. 40,41 In contrast, other authors have shown a low frequency of MLH1 methylation in oral carcinoma. 42,43 The authors reported that the differences between results may be a consequence of the different stages of development and progression in the studied samples.…”

MutSα and MutLα immunoexpression analysis in diagnostic grading of oral epithelial dysplasia and squamous cell carcinoma

“…[16][17][18] Promoter methylation and subsequent loss of hMLH1 expression has previously been associated with oral carcinogenesis and tumour progression. 14,29 Positive immunoreactivity for hMLH1 has great specificity and sensitivity for microsatellite instability detection, 30 and it is possible that previously reported intra-tumour heterogeneity in microsatellite instability for OSCC 31 may correlate with similar hMLH1 heterogeneity.…”

“…[14][15][16][17][18][19][20] Allelic imbalance in hMLH1 has been suggested to be an aetiological factor in head and neck carcinogenesis 20 and promoter methylation of this gene has been shown to be an early event in oral carcinogenesis. 14 Previous studies in this area have been limited to expression of hMLH1 and hMSH2 in either OPMD or squamous cell carcinoma.…”

Link between immunoexpression of hMLH1 and hMSH2 proteins and clinical-epidemiological aspects of actinic cheilitis

“…16 Some studies have evaluated the immunoexpression of these proteins in the degrees of dysplasia that AC may present, as well as in cancer, but no study has correlated the immunoexpression of these proteins with epidemiological and clinical aspects in AC. 5,6,8,17-19 This study is the first in the literature to analyze differences in expression of hMLH1 and hMSH2 proteins, correlating it with epidemiological and clinical characteristics of AC.…”