HMOCC-1, a human monoclonal antibody that inhibits adhesion of ovarian cancer cells to human mesothelial cells

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

“…This model histologically mimics the peritoneal lining, and allows us to recreate and examine key events in ovarian cancer metastasis including ovarian cancer cell adhesion, proliferation, and invasion 8,27 . Previously, limited 3D models have been established to investigate ovarian cancer interactions with the microenvironment 13,14,[21][22][23][24][25] , while this 3D organotypic model is the first to recreate the construction of the mesothelial-lining of the peritoneal cavity. The human omentum is collected from patients varying in age and health.…”

“…Historically, several 3D models have been developed to investigate ovarian cancer interactions with the microenvironment. Some of the first models studied ovarian cancer-ECM interface [17][18][19][20][21] , ovarian cancer-mesothelial cell communication 13,14,[21][22][23][24] , or both 25 (reviewed by us 26 ). Niedbala et al discovered that ovarian cancer cells display a quicker and firmer adhesion to ECM than to mesothelial cells or to plastic alone 25 .…”

Identification of Mono- and Disulfated N-Acetyl-lactosaminyl Oligosaccharide Structures as Epitopes Specifically Recognized by Humanized Monoclonal Antibody HMOCC-1 Raised against Ovarian Cancer

“…Production of HMOCC-1 antibody was described previously (15). Purified HMOCC-1 antibody was provided by Kyowa-Kirin Co. Ltd.…”

“…In this study, we determined the epitope carbohydrate structure recognized by the humanized monoclonal antibody HMOCC-1, which was raised against ovarian clear cell carcinoma RMG-I cells (15). A previous study showed that HMOCC-1 antigen is found in ovarian cancer tissues and in cell lines derived from ovarian cancer and is associated with a 100-kDa glycoprotein and that HMOCC-1 antigenic glycans were susceptible to N -glycanase, suggesting that some, if not all, HMOCC-1 antigen is carried by N -glycans (15).…”

“…A previous study showed that HMOCC-1 antigen is found in ovarian cancer tissues and in cell lines derived from ovarian cancer and is associated with a 100-kDa glycoprotein and that HMOCC-1 antigenic glycans were susceptible to N -glycanase, suggesting that some, if not all, HMOCC-1 antigen is carried by N -glycans (15). It was also shown previously that HMOCC-1 inhibited adhesion of RMG-I cells to cultured peritoneal mesometrial cells.…”