HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1

Pan, Zongfu; Fang, Qilu; Lu, Li; Zhang, Yiwen; Xu, Tao; Liu, Yujia; Xing, Zheng; Tan, Zhuo; Huang, Ping; Ge, Minghua

doi:10.1016/j.canlet.2020.12.026

Cited by 29 publications

(19 citation statements)

References 36 publications

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“…In a study of 4625 patients with solid tumors, overexpression of STMN1 was associated with poor overall survival (31). HN1, interacting with STMN1, reduces a-tubulin acetylation and promotes tumor progression through EMT (32). CAB39L is a tumor metabolism regulator with the functions of tumor suppressor (33).…”

Section: Discussionmentioning

confidence: 99%

The Key Gene Expression Patterns and Prognostic Factors in Malignant Transformation from Enchondroma to Chondrosarcoma

Wu¹,

Huang²,

Yu³

et al. 2021

Front. Oncol.

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Enchondroma (EC) is a common benign bone tumor. It has the risk of malignant transformation to Chondrosarcoma (CS). However, the underlying mechanism is unclear. The gene expression profile of EC and CS was obtained from Gene Expression Omnibus (GEO) database. The differentially expressed genes (DEGs) were identified using GEO2R. We conducted the enrichment analysis and constructed the gene interaction network using the DEGs. We found that the epithelial-mesenchymal transition (EMT) and the VEGFA-VEGF2R signaling pathway were more active in CS. The CD8+ T cell immunity was enhanced in CS I. We believed that four genes (MFAP2, GOLM1, STMN1, and HN1) were poor predictors of prognosis, while two genes (CAB39L and GAB2) indicated a good prognosis. We have revealed the mechanism in the tumor progression and identified the key genes that predicted the prognosis. This study provided new ideas for the diagnosis and treatment of EC and CS.

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Section: Discussionmentioning

confidence: 99%

The Key Gene Expression Patterns and Prognostic Factors in Malignant Transformation from Enchondroma to Chondrosarcoma

Wu¹,

Huang²,

Yu³

et al. 2021

Front. Oncol.

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“…HN1 reportedly promoted proliferation and metastasis of malignant cells in many human cancers. Recent research revealed that HN1 could increase STMN1 protein level by inhibiting its ubiquitination-mediated degradation to promote aggressiveness of anaplastic thyroid carcinoma [21]. In addition, previous work demonstrated that ectopic HN1 expression could increase degradation of β-catenin and resulted in disassociation of β-catenin and E-cadherin, which promoted progression of prostate cancer [22].…”

Section: Discussionmentioning

confidence: 99%

“…Hematological and neurological expressed (HN1), locating on chromosome 17q25.2, encodes a 16.5 kilodalton protein which is ubiquitously expressed and highly conserved [20]. Increasing evidences indicated that HN1 showed an elevated expression level and was associated with poor prognosis in many tumors [21][22][23]. HN1 has been proposed to act as a biomarker for diagnosis and prognosis of HCC [24].…”

Section: Introductionmentioning

confidence: 99%

HN1 Promotes Proliferation, Metastasis and Attenuates the Chemosensitivity of HCC Cells to Oxaliplatin by Inhibiting Degradation of HMGB1 Through Interacting With TRIM28

Wang

Yao

et al. 2021

Preprint

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Background: The oxaliplatin-based chemotherapy has revealed an encouraging therapeutic efficacy for advanced hepatocellular carcinoma patients. However, the development of resistance limits its clinical utilization. In addition, the chemotherapy resistance in HCC is usually accompanied with other malignant phenotypes, such as cell proliferation and metastasis, which together result in poor prognosis of HCC patients. Therefore, efforts should be made to explore potential regulators which fuel multiple events of HCC progression.Methods: The qRT-PCR, western blot, immunohistochemistry and immunofluorescence were performed to measure mRNA and protein expression. MTT assay, colony formation and Transwell assay were performed to evaluate cell proliferation and metastasis. Flow cytometry was performed to test cell apoptosis. Alkaline Comet assay was performed to measure DNA lesions. Transmission electron microscope analysis provided potent testimony of autophagy. The role of HN1 on the malignant phenotypes of hepatoma carcinoma was demonstrated in vitro and in vivo.Results: The immunohistochemistry analysis of HCC patient tissues revealed that the expression of HN1 was higher in HCC tissues compared to adjacent tissues and was associated with worse prognosis. In vitro, HN1 knockdown inhibited proliferation and metastasis of HCC cells, whereas HN1 overexpression promoted their proliferation and metastasis. In addition, we found that HN1 knockdown sensitized HCC cells to oxaliplatin, which is companied with deteriorated DNA damage and increased cell apoptosis in oxaliplatin-treated HCC cells. In vivo, HN1 knockdown inhibited the tumor growth and metastasis, and promoted the anti-cancer efficiency of oxaliplatin. Mechanically, HN1 prevented HMGB1 from ubiquitination and degradation via autophagy-lysosome pathway, which is related to its interaction with TRIM28, and overexpression of HMGB1 can restore the malignant phenotypes of HN1 knockdown in HCC cells. Furthermore, we found that HN1 can regulate cellular autophagy via HMGB1, which is important to tumor-promoting effect of HN1.Conclusions: In conclusion, we systemically revealed the multiple functions of HN1 in HCC progression and the underlying molecular mechanism, which indicated that HN1 could be a promising therapeutic target for HCC treatment.

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“…Ding et al ( 16) have revealed that circ-DONSON is upregulated in tumor tissues and its knockdown restrains cell proliferation, migration, and invasion in GC. The expression of the HN1 gene is commonly elevated in tumors and contributes to accelerated tumor progression in breast cancer, anaplastic thyroid carcinoma, prostate cancer, and hepatocellular carcinoma (17)(18)(19)(20). CircHN1, encoded by the HN1 gene, can promote proliferation, migration, and invasion, as well as inhibit apoptosis in GC (11,12).…”

Section: Gsk3a Reversed the Effects Of Mir-485-5p On Gc Cell Progressionmentioning

confidence: 99%

Downregulation of circular RNA circ-HN1 suppressed the progression of gastric cancer through the miR-485-5p/GSK3A pathway

Zhang

Jiang

2022

Bioengineered

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Gastric cancer (GC) is a malignancy with high incidence and mortality globally. Circular RNAs (circRNAs) are reported to regulate cellular processes in human diseases, including GC.Herein, the functions of circ-HN1 and its molecular mechanisms were investigated. circ-HN1, miR-485-5p, and GSK3A levels in GC were measured using Real time-quantitative polymerase chain reaction (RT-qPCR). Cell proliferation was analyzed using cell counting kit-8 (CCK-8) and colony formation assays. Meanwhile, the migration and invasion abilities were analyzed using the transwell assay. The targeted relationship was confirmed using a luciferase reporter assay and an RNA pull-down assay. In both GC tissues and cells, circ-HN1 expression was upregulated, and its silencing suppressed cellular processes. Moreover, circ-HN1 served as a sponge of miR-485-5p, which was reduced in patients with GC and negatively regulated by circ-HN1 in GC cells. Inhibition of miR-485-4p abolished the biological functions induced by the silencing of circ-HN1. Additionally, miR-485-5p targeted GSK3A in GC, whose expression was elevated in tumor tissues and was negatively correlated with miR-485-5p in tumor cells. GSK3A rescued the inhibition of miR-485-5p in the cellular processes. In conclusion, silencing of the circ-HN1-miR-485-5p-GSK3A regulatory network inhibited GC cell proliferation, migration, and invasion, suggesting that circ-HN1 is a potential target for GC therapy.

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HN1 promotes tumor growth and metastasis of anaplastic thyroid carcinoma by interacting with STMN1

Cited by 29 publications

References 36 publications

The Key Gene Expression Patterns and Prognostic Factors in Malignant Transformation from Enchondroma to Chondrosarcoma

The Key Gene Expression Patterns and Prognostic Factors in Malignant Transformation from Enchondroma to Chondrosarcoma

HN1 Promotes Proliferation, Metastasis and Attenuates the Chemosensitivity of HCC Cells to Oxaliplatin by Inhibiting Degradation of HMGB1 Through Interacting With TRIM28

Downregulation of circular RNA circ-HN1 suppressed the progression of gastric cancer through the miR-485-5p/GSK3A pathway

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