HNEAP Regulates Necroptosis of Cardiomyocytes by Suppressing the m⁵C Methylation of Atf7 mRNA

Abstract

PIWI‐interacting RNAs (piRNAs) are highly expressed in various cardiovascular diseases. However, their role in cardiomyocyte death caused by ischemia/reperfusion (I/R) injury, especially necroptosis, remains elusive. In this study, a heart necroptosis‐associated piRNA (HNEAP) is found that regulates cardiomyocyte necroptosis by targeting DNA methyltransferase 1 (DNMT1)‐mediated 5‐methylcytosine (m5C) methylation of the activating transcription factor 7 (Atf7) mRNA transcript. HNEAP expression level is significantly elevated in hypoxia/reoxygenation (H/R)‐exposed cardiomyocytes and I/R‐injured mouse hearts. Loss of HNEAP inhibited cardiomyocyte necroptosis and ameliorated cardiac function in mice. Mechanistically, HNEAP directly interacts with DNMT1 and attenuates m5C methylation of the Atf7 mRNA transcript, which increases Atf7 expression level. ATF7 can further downregulate the transcription of Chmp2a, an inhibitor of necroptosis, resulting in the reduction of Chmp2a level and the progression of cardiomyocyte necroptosis. The findings reveal that piRNA‐mediated m5C methylation is involved in the regulation of cardiomyocyte necroptosis. Thus, the HNEAP‐DNMT1‐ATF7‐CHMP2A axis may be a potential target for attenuating cardiac injury caused by necroptosis in ischemic heart disease.

Recent advances of PIWI‐interacting RNA in cardiovascular diseases

“…HNEAP, linked to cardiac necrosis piRNA, enhances Atf7 expression via its interaction with DNMT1, thereby diminishing the presence of necrosis inhibitors like Chmp2a, which leads to cardiomyocyte necrosis. Reveals the HNEAP‐DNMT1‐ATF7‐CHMP2A shaft in the regulation function of cardiomyocyte necrosis 103 (Figure 5A ). Meanwhile, AVCAPIR binds the m 6 A demethylase FTO and inhibits its activity, resulting in the hypermethylation of CD36 mRNA downstream of FTO.…”