HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behçet's Disease

Chen, Peng; Zhang, Chunyan; Liu, Chen; Zhang, Liyun; Yang, Chunhe; Chen, Guangyu; Ma, Ding; Tian, Yaping; Du, Hongwu

doi:10.1016/j.ebiom.2016.03.009

Cited by 6 publications

(5 citation statements)

References 34 publications

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“…The expression and purification methods were the same as previously described . Briefly, PD2 and p50 were overexpressed in Escherichia coli BL 21.…”

Section: Methodsmentioning

confidence: 99%

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Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening

Wang

Chen

et al. 2022

ACS Pharmacol. Transl. Sci.

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Andrographolide (andro) and its derivatives have been reported to have antitumor activity by arresting the cell cycle. However, the more precise mechanism has been controversial. Here, a proteome chip was used to screen drug targets in cells, and we discovered that andro can bind to PDCD2 (PD2), which has been shown to be associated with the cell cycle and mRNA nuclear export. Then, RNA-binding protein immunoprecipitation for PD2 was used to detect the quantity of cell cycle-related mRNAs, and the nuclear distribution difference analyses of these mRNAs in tumor cells after andro intervention, followed by systematic experiments, were performed to assess the downstream effects of this event in vivo and in vitro. Thus, the target spectrum of andro was revealed at the level of the human proteome chip for the first time, and this work demonstrated that andro, through targeting PD2, blocks the nuclear output of CDK mRNAs in the nucleus of tumor cells, further reduces the expression of cell CDK proteins, and finally causes tumor cell cycle arrest in phenotype and tumor tissue growth arrest in vivo.

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“…The expression and purification methods were the same as previously described . Briefly, PD2 and p50 were overexpressed in Escherichia coli BL 21.…”

Section: Methodsmentioning

confidence: 99%

“…The expression and purification methods were the same as previously described. 26 Briefly, PD2 and p50 were overexpressed in Escherichia coli BL 21. After the cells were lysed using ultrasonic lysis in phosphate-buffered saline (PBS) (pH = 7.4), the supernatant was purified with Ni-NTA resin (Cwbiotech, Beijing, China).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening

Wang

Chen

et al. 2022

ACS Pharmacol. Transl. Sci.

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“…This method was applied in our recent studies [ 28 ]. Briefly, the target protein (recombinant Annexin A1) band was cutoff from SDS/PAGE gel.…”

Section: Methodsmentioning

confidence: 99%

Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease

et al. 2018

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The autoantibodies profile of Behçet’s disease (BD) is yet incompletely understood. Annexins are a family of highly conserved proteins which are involved in some human autoimmune diseases. Autoantibodies directed toward Annexin A1 and A2 are involved in BD pathology, but correlation in their clinical role is controversial. The aim of our study is to estimate and evaluate the expression correlation between Annexin A1 and A2 autoantibodies in BD patients. We have designed and implemented different technical approaches to prove the hypothesis. First, bioinformatics tools such as amino acid sequence alignment, epitope prediction analysis, and 3D structural comparison were performed to find out the correlation between Annexin A1 and A2. Second, amplification of the corresponding gene by RT-PCR, then cloning, and purification techniques were applied to acquire the recombinant Annexin A1. Third, the target protein band was excised from gel electrophoresis, digested with trypsin, and analyzed by MALDI-TOF/TOF. Finally, in-house ELISA was developed to determine the induced anti-Annexin A1 autoantibodies in BD patients. Obtained results demonstrated that the BD serum reactivity against recombinant Annexin A1 was significantly higher as compared with healthy control (HC) (P<0.001). Moreover, bioassay results of Annexin A1 and A2 also showed the presence, absence, and independent coexistence of autoantibodies, when reacted with BD sera. In conclusion, Annexin A1 has a similar immunogenic expression and correlation with its analog Annexin A2 and their association may be a novel immune target of BD in Han Chinese population.

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“…The tissue sections of the ulcers isolated from BD patients revealed that serum IgA autoantibodies colocalized with monoclonal antibodies against hnRNP A2/B1 (Cho, Zheng, Cho, et al, 2013). In addition, hnRNP C1/C2 and hnRNP A1 were also identified as potential autoantigens in BD and the positivity of hnRNP A1 autoantibodies was associated with the deep vein thrombosis (Chen et al, 2016; Hussain et al, 2018).…”

Section: Hnrnps Are Autoantigens In Autoimmune Diseases and Associate...mentioning

confidence: 99%

Undervalued and novel roles of heterogeneous nuclear ribonucleoproteins in autoimmune diseases: Resurgence as potential biomarkers and targets

Chen

Luo

et al. 2023

WIREs RNA

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Autoimmune diseases are mainly characterized by the abnormal autoreactivity due to the loss of tolerance to specific autoantigens, though multiple pathways associated with the homeostasis of immune responses are involved in initiating or aggravating the conditions. The heterogeneous nuclear ribonucleoproteins (hnRNPs) are a major category of RNA‐binding proteins ubiquitously expressed in a multitude of cells and have attracted great attentions especially with their distinctive roles in nucleic acid metabolisms and the pathogenesis in diseases like neurodegenerative disorders and cancers. Nevertheless, the interplay between hnRNPs and autoimmune disorders has not been fully elucidated. Virtually various family members of hnRNPs are increasingly identified as immune players and are pertinent to all kinds of immune‐related processes including immune system development and innate or adaptive immune responses. Specifically, hnRNPs have been extensively recognized as autoantigens within and even beyond a myriad of autoimmune diseases, yet their diagnostic and prognostic values are seemingly underestimated. Molecular mimicry, epitope spreading and bystander activation may represent major putative mechanisms underlying the presence of autoantibodies to hnRNPs. Besides, hnRNPs play critical parts in regulating linchpin genes expressions that control genetic susceptibility, disease‐linked functional pathways, or immune responses by interacting with other components particularly like microRNAs and long non‐coding RNAs, thereby contributing to inflammation and autoimmunity as well as specific disease phenotypes. Therefore, comprehensive unraveling of the roles of hnRNPs is conducive to establishing potential biomarkers and developing better intervention strategies by targeting these hnRNPs in the corresponding disorders.This article is categorized under: RNA in Disease and Development > RNA in Disease RNA Interactions with Proteins and Other Molecules > Protein‐RNA Interactions: Functional Implications

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HnRNP A1 is Involved in Deep Vein Thrombosis Patients with Behçet's Disease

Cited by 6 publications

References 34 publications

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening

Identification of PDCD2 as a Candidate Target of Andrographolide That Arrests the Tumor Cell Cycle by Human Proteome-Scale Screening

Expression correlates between Annexin A1 and A2 autoantibodies in patients with Behçet’s disease

Undervalued and novel roles of heterogeneous nuclear ribonucleoproteins in autoimmune diseases: Resurgence as potential biomarkers and targets

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