Hodgkin and sternberg‐reed cells contain antigens specific to late cells of granulopoiesis

Abstract

The results of a recent investigation, in which an antiserum specifically directed against Hodgkin (H) and Sternberg-Reed (SR) cells was prepared, indicated the existence of a granulocytic cell-specific antigen on H and SR cells. In the present study, a large series of biopsies from patients with Hodgkin's disease were subjected to immunostaining with monoclonal mouse antibodies raised against acute myelomonocytic leukemia (AMML) cells. Among seven hybrids that secreted antibodies showing reactivity to AMML cells but not to Daudi cells, there were three (TU5, TU6 and TU9) whose antibodies selectively stained formalin resistant antigens in cells of granulopoiesis. The strongest staining was found in the more mature cells; only a few promyelocytes stained very faintly with TU9, H and SR cells showed distinct staining for TU9 in 57 (76%) of the 75 tested cases of Hodgkin's disease, whereas TU5 and TU6-reactive H and SR cells were found in only 35 cases (47%). All cases of the nodular sclerosis type and almost all cases of the mixed cellularity type contained TU9-reactive H and SR cells, although the percentage varied from case to case. TU9-reactive H and SR cells were demonstrated in nine of 12 cases of the lymphocyte depletion type and in eight of 21 cases of the lymphocyte predominance type. The presence of granulocytic cell-specific antigens in H and SR cells in most of the cases of Hodgkin's disease suggest that (1) H and SR cells (including the lacunar cell variant) are not heterogeneous, but rather homogeneous in origin and nature, at least in a majority of cases, and (2) H and SR cells are more closely related to cells of the granulocytic cell lineage than to any other type of cell of the hemato-lymphoid system.

Control of human B cell tumor growth in severe combined immunodeficiency mice by monoclonal anti-B cell antibodies.

“…Sections 2 Mm thick were stained with hematoxylin and eosin. A three-stage indirect immunoperoxidase method ( 19) The three patients received same therapeutic protocol using anti-B cell monoclonal antibodies anti-CD21 and anti-CD24. The patients from whom the cell lines (BLCL) 48 and 22 were derived were cured but the patient from whom BLCL 41 was derived died rapidly from the disease progression (25).…”

Immunopathology of Hodgkin’s Disease

“…Anti-Leu M-I consistently reacts with Reed-Sternberg cells of all types of Hodg kin's disease with the general exception of the lymphocyte predominant (nodular lym phocytic and histiocytic) form [12,13,17,[39][40][41]43], Leu M-l, unlike other lymphoid antigens, is not destroyed by formalin fixa tion and paraffin embedding and is detect able in deparaffinized as well as snap frozen tissue [ 17,40,41 ]. Leu M-1 is not detected in most cases of lymphocyte predominant (no dular lymphocytic and histiocytic) Hodg kin's disease [13,17,40,41], but has been shown to be present in sialylated form [42], The expression of a different form of the antigen in this type of Hodgkin's disease is consistent with the impression of some de gree of heterogeneity in the Hodgkin's cell phenotype [42], Leu M-l expression is not unique to Hodgkin's disease.…”

Intraglomerular Expression of MHC Class II and Ki-67 Antigens and Serum γ-lnterferon Levels in IgA Nephropathy

“…The proliferative nuclear antigen Ki-67, present in all cell cycle phases except G0and early G|, is a useful marker for the detection of proliferative cells in vivo [13,14]. Ki-67 is also found in activated T cells in association with the expression of HLA-DR (Ial) in viral infections [15].…”