Hodgkin’s lymphoma RNA-transfected dendritic cells induce cancer/testis antigen-specific immune responses

Winkler, Carolin; Steingrube, Daniel Sebastian; Altermann, Wolfgang; Schlaf, Gerald; Max, Daniela; Kewitz, Stefanie; Emmer, Alexander; Kornhuber, Malte; Banning-Eichenseer, Ursula; Staege, Martin S.

doi:10.1007/s00262-012-1239-z

Cited by 31 publications

(53 citation statements)

References 49 publications

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“…No significant results were received for LIPI peptide SLS (p = 0.26) presented by LCL. Significant IFNγ-release was gained for CCND1 peptides LLG (p = 0.02) and RLT (p = 0.02) and for LIPI peptides LDY (p = 0.03) and NLL (p = 0.03) as well as for HLA-A2-positive [21][22][23] A673-cells (p = 0.01).…”

Section: B Cells (Lcl)mentioning

confidence: 91%

“…CD8-enriched PBMC intended to be analyzed by IFNγ ELISPOT were primed with SK-N-MC lysate-pulsed DC. IFNγ release was measured one week after first re-stimulation during exposition to peptides derived from LIPI or CCND1 presented on allogeneic HLA-A2 positive LCL or to HLA-A2 positive A673 or HLA-A2 negative 21 SK-N-MC tumor cells. Negative controls include CD8-enriched PBMC without re-stimulation during IFNγ ELISPOT.…”

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

“…HLA-haplotypes of EFT cells lines were determined as described. 21 The HLA-A2-positive Epstein Barr virus-immortalized LCL A2+2310 was established in our laboratory 38 and maintained in X-VIVO 15 Medium (Lonza). LCL were used as APC in IFNγ-ELISPOT assay (Becton-Dickinson) or for restimulation of PBMC.…”

Section: Cells and Cell Culture Pbmc DC Eft Cell Lines A673mentioning

confidence: 99%

“…Calculations were performed with the MAfilter software. 21 In a second approach, already known CTA that are listed in the CTA-database of the Ludwig Institute for Cancer Research 7 were checked for expression in EFT in microarray-data sets. Instead of using a fixed threshold with supposed expression of a gene when this threshold is exceeded, expression of a known CTA was assessed employing a relative threshold.…”

Section: Cells and Cell Culture Pbmc DC Eft Cell Lines A673mentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Ewing sarcoma associated cancer/testis antigens

Mahlendorf

Staege

2013

Cancer Biology & Therapy

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Section: B Cells (Lcl)mentioning

confidence: 91%

Section: Disclosure Of Potential Conflicts Of Interestmentioning

confidence: 99%

Section: Cells and Cell Culture Pbmc DC Eft Cell Lines A673mentioning

confidence: 99%

Section: Cells and Cell Culture Pbmc DC Eft Cell Lines A673mentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Ewing sarcoma associated cancer/testis antigens

Mahlendorf

Staege

2013

Cancer Biology & Therapy

Self Cite

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“…All cell files were processed together using the robust microarray analysis algorithm with Expression Console 1.1 (Affymetrix). For the identification of differentially expressed genes, probe sets were prefiltered using MAFilter [31]. Cluster analysis was performed with Genesis [32].…”

Section: Gene Expression Analysismentioning

confidence: 99%

Stimulation of the hypoxia pathway modulates chemotherapy resistance in Hodgkin’s lymphoma cells

et al. 2015

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Hodgkin's lymphoma (HL) is a malignant disease of the lymphatic system. The therapy has been improved during the last decades but there are still patients who cannot be cured, and the therapy is associated with several adverse late effects. Therefore, we asked which genes might be involved in the chemotherapy resistance of HL cells. We observed that HL cells became more resistant against cisplatin after treatment with cobalt chloride. Therefore, we analyzed which genes were differentially expressed between cells incubated in medium with or without cobalt chloride. We found several genes which were up- or downregulated in the presence of cobalt chloride and might be involved in the modulation of chemotherapy resistance. Cobalt chloride is a hypoxia-mimetic agent. Therefore, we tested chemo-resistance and gene expression of HL cells under hypoxic conditions and confirmed the results from the cobalt chloride experiments. Taken together, activation of the hypoxia pathway led to altered gene expression and drug resistance of HL cells. Differentially expressed genes might be interesting targets for the development of future treatment strategies against drug-resistant HL.

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Histone variants: critical determinants in tumour heterogeneity

et al. 2019

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Malignant cell transformation could be considered as a series of cell reprogramming events driven by oncogenic transcription factors and upstream signalling pathways. Chromatin plasticity and dynamics are critical determinants in the control of cell reprograming. An increase in chromatin dynamics could therefore constitute an essential step in driving oncogenesis and in generating tumour cell heterogeneity, which is indispensable for the selection of aggressive properties, including the ability of cells to disseminate and acquire resistance to treatments. Histone supply and dosage, as well as histone variants, are the best-known regulators of chromatin dynamics. By facilitating cell reprogramming, histone under-dosage and histone variants should also be crucial in cell transformation and tumour metastasis. Here we summarize and discuss our knowledge of the role of histone supply and histone variants in chromatin dynamics and their ability to enhance oncogenic cell reprogramming and tumour heterogeneity.

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Hodgkin’s lymphoma RNA-transfected dendritic cells induce cancer/testis antigen-specific immune responses

Cited by 31 publications

References 49 publications

Characterization of Ewing sarcoma associated cancer/testis antigens

Characterization of Ewing sarcoma associated cancer/testis antigens

Stimulation of the hypoxia pathway modulates chemotherapy resistance in Hodgkin’s lymphoma cells

Histone variants: critical determinants in tumour heterogeneity

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