Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma

Abstract

Holliday junction recognition protein (HJURP) is involved in the regulation of mortality in various cell types, including renal cell carcinoma (RCC) cells. The specific mechanisms by which HJURP regulates RCC cell apoptosis and the cell cycle have not been previously investigated, to the best of our knowledge. In the present study, the expression of HJURP in RCC tissues and adjacent paracancerous renal tissue, as well as in RCC cell lines, was analyzed using reverse transcription-quantitative PCR and western blot analysis. The A498 RCC cells were transfected with an HJURP overexpression vector, which resulted in reduced proliferation, as demonstrated using immunofluorescence staining, a Cell Counting Kit-8 assay and a colony formation assay. Flow cytometry and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labelling assays were used to determine the effect of HJURP on the cell cycle and apoptosis of RCC cells. Proteins associated with the reactive oxygen species (ROS) status were analyzed using western blot analysis. The expression of HJURP was lower in RCC tissues and cells compared with that in the adjacent paracancerous renal tissues and control cells. Furthermore, overexpression of HJURP resulted in a decrease in cell viability and proliferation in vitro. Overexpression of HJURP resulted in cell cycle arrest at the G0/G1 phase, cell apoptosis and an increase in ROS stress. In addition, the phosphorylated/total sirtuin 1 (SIRT1) protein ratio was decreased, whereas the expression of peroxisome proliferator-activated receptor (PPAR)γ was increased in the HJURP-overexpressing RCC cells. In clinical practice, decreased HJURP expression may be associated with poor prognosis in patients with RCC. These results suggest that HJURP may regulate cell apoptosis and proliferation in RCC cells and this may be mediated by PPARγ/SIRT1. Thus, HJURP may be used as a predictor of prognosis in patients with RCC.

m1A Regulatory gene signatures are associated with certain immune cell compositions of the tumor microenvironment and predict survival in kidney renal clear cell carcinoma

“…Our findings are also well in line with the hot nature of RCC tumor that generally show high levels of immune infiltration. Consistent with this viewpoint, a study conducted by Zhang et al showed a significant correlation between the Holliday junction recognition protein (HJURP) and the progression of tumors across various malignancies, frequently indicating a poor prognosis [ 51 ]. This association extends to the infiltration of diverse immune cells and the expression of a broad array of genetic markers associated with immune cells.…”

Identification and verification of the role of crucial genes through which methionine restriction inhibits the progression of colon cancer cells

“…In addition, studies have shown that HJURP can promote epithelial-mesenchymal transition of tumor cells by regulating the Wnt/β-catenin pathway and Sphingosine kinase 1 (40,41). In addition, studies by Cao et al (42) and Yuan et al (43) successively confirmed that HJURP affects the oxidative stress and cell cycle arrest of tumor cells by regulating the peroxisome proliferator-activated receptor γ-sirtuin 1 feedback loop. In the present study, it was confirmed that E2F1, MIR17HG, FANCI and HJURP are highly expressed in colon cancer tissues.…”

To Identify and Verify the Role and Crucial Genes of Methionine Restriction in Inhibiting the Progression of Colon Cancer Cells

“…In addition, studies have shown that HJURP can promote epithelial-mesenchymal transition of tumor cells by regulating the Wnt/β-catenin pathway and SPHK1 [44,45] . Not only that, studies by Yuan et al and Cao et al have successively con rmed that HJURP affects the oxidative stress and cell cycle arrest of tumor cells by regulating the peroxisome proliferator-activated receptorγ-sirtuin1 feedback loop [46,47] . In our research, we con rmed that E2F1, MIR17HG, FANCI, and HJURP are highly expressed in colon cancer tissues.…”