Hollow-Fiber Flow Field-Flow Fractionation for Whole Bacteria Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Reschiglian, Pierluigi; Zattoni, Andrea; Cinque, Leonardo; Roda, Barbara; Piaz, Fabrizio Dal; Roda, Aldo; Moon, Myeong Hee; Min, Byung Ryul

doi:10.1021/ac0353379

Cited by 60 publications

(48 citation statements)

References 34 publications

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“…The variability of profile patterns within the fractions was found to be insignificant. Hollow fiber FFF was found useful for eliminating non-cellular components from the sample (Reschiglian et al, 2004). Moreover, the separation of B. subtilis from E. coli was also achieved.…”

Section: F Sample Fractionationmentioning

confidence: 99%

Sample preparation methods for MALDI‐MS profiling of bacteria

Šedo¹,

Sedláček²,

Zdráhal³

2010

Mass Spectrometry Reviews

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Direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.

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Section: F Sample Fractionationmentioning

confidence: 99%

Sample preparation methods for MALDI‐MS profiling of bacteria

Šedo¹,

Sedláček²,

Zdráhal³

2010

Mass Spectrometry Reviews

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“…With this respect, several technological approaches have been proposed, such as accurate protocols standardization, bio-inspired bacteria purification systems, coupling with separative techniques, high-resolution MS methods, and bioinformatic tools [92][93][94][95]. Employing the pathogenic bacteria E. coli O157:H7 and Yersinia enterocolitica and the non-pathogenic E. coli MC1061 strain as model samples, we recently proposed the use of chemometric tools to extract relevant information, useful for comparing the fingerprint MALDI-TOF spectra of unknown bacteria with a spectral library, to accomplish robust bacteria identification and classification [96].…”

Section: Mass Spectrometrymentioning

confidence: 99%

“…Therefore, complementary separative techniques have been proposed to prepare the sample and provide a fraction enriched in the bacteria of interest for MALDI-TOF analysis. With this respect, field-flow fractionation (FFF) techniques have proved a useful non-invasive sample preparation tool [95], as better described in the paragraph "Bacteria separation to assist bioassays".…”

Section: Mass Spectrometrymentioning

confidence: 99%

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

et al. 2012

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Foodborne illnesses caused by pathogenic bacteria represent a widespread and growing problem to public health, and there is an obvious need for rapid detection of food pathogens. Traditional culture-based techniques require tedious sample workup and are time-consuming. It is expected that new and more rapid methods can replace current techniques. To enable large scale screening procedures, new multiplex analytical formats are being developed, and these allow the detection and/or identification of more than one pathogen in a single analytical run, thus cutting assay times and costs. We review here recent advancements in the field of rapid multiplex analytical methods for foodborne pathogenic bacteria. A variety of strategies, such as multiplex polymerase chain reaction assays, microarray-or multichannel-based immunoassays, biosensors, and fingerprint-based approaches (such as mass spectrometry, electronic nose, or vibrational spectroscopic analysis of whole bacterial cells), have been explored. In addition, various technological solutions have been adopted to improve detectability and to eliminate interferences, although in most cases a brief pre-enrichment step is still required. This review also covers the progress, limitations and future challenges of these approaches and emphasizes the advantages of new separative techniques to selectively fractionate bacteria, thus increasing multiplexing capabilities and simplifying sample preparation procedures.

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“…Drawbacks to this approach include the requirement for a highly concentrated sample (10 5 to 10 7 cells/ml for whole-cell analysis), the need to develop complex spectral fingerprints for every target agent, and the possible lack of specificity in complex matrices or mixtures of targets. Flow fieldflow fractionation is being investigated as one way of increasing specificity by separating particles in a complex matrix prior to analysis by MALDI-TOF/mass spectrometry (173,240).…”

Section: Chemical and Physical Detectionmentioning

confidence: 99%

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

et al. 2005

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SUMMARY Recent events have made public health officials acutely aware of the importance of rapidly and accurately detecting acts of bioterrorism. Because bioterrorism is difficult to predict or prevent, reliable platforms to rapidly detect and identify biothreat agents are important to minimize the spread of these agents and to protect the public health. These platforms must not only be sensitive and specific, but must also be able to accurately detect a variety of pathogens, including modified or previously uncharacterized agents, directly from complex sample matrices. Various commercial tests utilizing biochemical, immunological, nucleic acid, and bioluminescence procedures are currently available to identify biological threat agents. Newer tests have also been developed to identify such agents using aptamers, biochips, evanescent wave biosensors, cantilevers, living cells, and other innovative technologies. This review describes these current and developing technologies and considers challenges to rapid, accurate detection of biothreat agents. Although there is no ideal platform, many of these technologies have proved invaluable for the detection and identification of biothreat agents.

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Hollow-Fiber Flow Field-Flow Fractionation for Whole Bacteria Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Cited by 60 publications

References 34 publications

Sample preparation methods for MALDI‐MS profiling of bacteria

Sample preparation methods for MALDI‐MS profiling of bacteria

Recent developments in rapid multiplexed bioanalytical methods for foodborne pathogenic bacteria detection

Current and Developing Technologies for Monitoring Agents of Bioterrorism and Biowarfare

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