Hollow-fibre affinity cell separation system for CD34+ cell enrichment

Nordon, Robert E.; Haylock, David N.; Gaudry, Leonie; Schindhelm, Klaus

doi:10.1002/(sici)1097-0320(19960801)24:4<340::aid-cyto5>3.0.co;2-j

Cited by 12 publications

(7 citation statements)

References 19 publications

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“…The physical criteria developed for uniform fiber flow will assist in the development of large-scale systems. Experimental data using a small scale system (surface area, 220 cm') have shown that CD34' cell yields are adequate if the device is loaded with not more than Y2 million cells/cm2 of lumenal surface area (17). Therefore, a large-scale system (lo1' cells) would require a surface area of 2 m2 (e.g., 10,000 fibers; device length, 32 cm; fiber bundle radius, 2 cm).…”

Section: Resultsmentioning

confidence: 99%

“…A hollow fiber immunoadsorption system (surface area, 220 cm2) was developed for the purification of CD34' cells from clinical material (17). Monoclonal antibody to the CD34 antigen was covalently coupled to the lumenal surface of Cuprophan (regenerated cellulose) hollow fiber modules (AKZO Nobel Faser AG Membrana, Wuppertal, Germany).…”

Section: Re N O R D O N a N D K Schindhelmmentioning

confidence: 99%

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Design of Hollow Fiber Modules for Uniform Shear Elution Affinity Cell Separation

Nordon

Schindhelm

1997

Artificial Organs

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Abstrad: Large-scale monoclonal antibody based systems for the selection of cell subsets will play a prominent role in the development of hematotherapy and graft engineering. Hollow fiber systems for affinity cell separation rely on the generation of uniform fluid shear stress at the lumenal attachment interface. Potential mechanisms for nonuniformity of lumenal wall shear stress are fiber wall permeation fluxes driven by the pressure gradient along individual fibers and the influence of inlet header dynamic pressure on the radial distribution of axial flow within the fiber module. Dimensional analysis and numerical solution of the flow field within the lumen of a hollow fiber module illustrate the main physical criteria for design of hollow fiber modules. There will be a nearly uniform distribution of flow within the fiber bundle provided that the dynamic inlet pressure is small in comparison with the pressure drop along fibers. Fiber wall permeation fluxes will have a negligible effect on axial flow rate for nonporous membranes such as Cuprophan.

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Section: Resultsmentioning

confidence: 99%

Section: Re N O R D O N a N D K Schindhelmmentioning

confidence: 99%

Design of Hollow Fiber Modules for Uniform Shear Elution Affinity Cell Separation

Nordon

Schindhelm

1997

Artificial Organs

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“…They demonstrated that cellulose particles coated with SCF are colocalized with the receptor for SCF (c‐kit) on B6SutA cells. Subsequent work will examine the potential role for immobilized ligands using hollow‐fiber‐based systems for cell selection and expansion (16, 17).…”

Section: Discussionmentioning

confidence: 99%

Hollow‐fiber assay for ligand‐mediated cell adhesion

et al. 2003

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Background:The investigation of receptor-ligand interactions in the cellular context presents significant technical challenges, first, to immobilize the ligand in a manner that preserves functional properties and, second, to relate ligand properties to cell adhesion and other cellular processes. Methods: Ligand-mediated cell adhesion was characterized by the development of a cellulose hollow-fiber adhesion assay in which ligand (protein A) was immobilized onto the cellulose membrane as a recombinant fusion protein containing a cellulose-binding domain affinity tag. Modules containing single cellulose hollow fibers were connected to a micro-flow system for cell deposition and detachment with fluid shear stress. The cell adhesion process that occurred inside a segment of hollow fiber was observed in real time by using an inverted microscope equipped with a CCD camera and digital frame grabber.

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“…A number of approaches to controlling this reaction using mAbs have been applied, including removal of the T-cells from the inoculum before transplantation [22], isolation or enrichment of stem cells before transplantation [23][24][25] and treatment of the recipient with mAb to inhibit T-cell function (thus inhibiting both graft versus-host and graft rejection) [26]. A particularly informative series of studies has utilised various forms of an antibody, CAMPATH-1, against CD52 [27,28].…”

Section: Immunosuppressionmentioning

confidence: 99%

Human leukocyte differentiation antigens as therapeutic targets: the CD molecules and CD antibodies

Zola¹

2001

Expert Opinion on Biological Therapy

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The cell membrane presents an attractive target in a number of different disease situations. Most obviously, malignant cells may be killed by damaging their cell membranes. There are also more subtle, though effective, ways of rendering cells harmless by engaging proteins at the cell surface. The cells of the immune system may be targeted, for example to stop a damaging immune reaction, such as acute inflammation or rejection of a transplanted organ. If we are to make the best use of the opportunities to modulate disease by targeting the cell membrane, we need a detailed understanding of the many proteins, glycoproteins and glycolipids that are attached to or inserted in the cell membrane. The CD (cluster of differentiation) Workshops, more properly known as the HLDA (Human Leukocyte Differentiation Antigens) Workshops have, since 1982, focussed on the study of the membrane molecules of leukocytes, including the major cells of the immune system and malignant cells derived from them. The scope has extended to molecules on endothelium which are important in interaction with leukocytes. Many of the molecules characterised as leukocyte antigens are also expressed on other tissue. The approaches developed by the HLDA Workshops are useful in the study of the molecular composition and function of cells of other organ systems. Some of the antibodies produced in order to study the CD molecules have proved useful as therapeutic agents. This review describes the CD system, how it has developed and what it means and introduces the field of therapy based on antibodies against CD or similar molecules. The author is responsible for organising the next (8th) HLDA Workshop and invites readers to suggest ways in which the therapeutic relevance of the Workshop may be enhanced.

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Hollow-fibre affinity cell separation system for CD34+ cell enrichment

Cited by 12 publications

References 19 publications

Design of Hollow Fiber Modules for Uniform Shear Elution Affinity Cell Separation

Design of Hollow Fiber Modules for Uniform Shear Elution Affinity Cell Separation

Hollow‐fiber assay for ligand‐mediated cell adhesion

Human leukocyte differentiation antigens as therapeutic targets: the CD molecules and CD antibodies

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