Hollow fibre cell fishing with high performance liquid chromatography for screening bioactive anthraquinones from traditional Chinese medicines

Screening and quantification of anticancer compounds in traditional Chinese medicine by hollow fiber cell fishing and hollow fiber liquid/solid‐phase microextraction

“…The process of cell seeding on HF was administered as described in Ref. . Subsequently, the suspension liquid in the fiber seeded cells was discarded, new nutrient solution was injected into the fiber with a microsyringe, and both ends of the fiber were tied.…”

“…LPME and SPME based on a hollow fiber (HF) have been widely used in the extraction, concentration, and purification of active compounds in TCMs because of the low organic solvent consumption, simplicity, speed, high enrichment factor, and cost‐effectiveness of these techniques. Recently, our research group proposed hollow fiber cell fishing (HFCF) with HPLC on the basis of the previous research and introduced the approach to screen, fish, and identify anticancer active compound groups from TCMs . Soon after, we used HFs filled with cell membranes, organelles and receptors, respectively, to investigate the binding sites and targets of potential anticancer active groups in TCMs .…”

Analysis of α-amylase inhibitor from corni fructus by coupling magnetic cross-linked enzyme aggregates of α-amylase with HPLC–MS

“…Therefore, it is necessary to develop rapid and effective approaches for screening of active compounds from natural products [6]. In recent years, various screening methods like cell membrane chromatography, ultrafiltration and functionalized magnetic nanoparticles have been developed to screen active compounds from natural products [7,8].…”

Overview of online two‐dimensional liquid chromatography based on cell membrane chromatography for screening target components from traditional Chinese medicines

“…However, complex composition, lack of scientific knowledge about their mechanism of action, targets in body, screening and quantification techniques restrict the use and acceptability of these holistic medicines . Over the past few decades different screening techniques have been developed for the screening of lead compounds include but are not limited to screening of bioactive compounds with liposomes , hollow fiber cell fishing with liquid chromatography (HFCF–HPLC) , direct drug screening methods based on target receptors or enzymes , and virtual screening using computer‐assisted drug design (CADD) . However, there are few efficient techniques suitable for TCM research.…”

“…However, complex composition, lack of scientific knowledge about their mechanism of action, targets in body, screening and quantification techniques restrict the use and acceptability of these holistic medicines [3]. Over the past few decades different screening techniques have been developed for the screening Correspondence: Professor Sicen Wang, School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, Shaanxi, China E-mail: wangsc@mail.xjtu.edu.cn; wangsicen@gmail.com Fax: +86- Abbreviations: DAD, Diode array detector; FGFR-4, Fibroblast growth factor receptor-4; HSC-T6, Hepatic stellate cells-6; H1R, Histamine receptor-1; SMMC-7721, Human hepatoma cell lines SMMC-7721; HepG2, Human hepatocellular carcinoma cells HepG2; HEK 293, Human embryonic kidney cells 293; MTT, 3-(4,5-dimethyl-2-thiazolyl)-2,5diphenyl-2-H-tetrazolium bromide; RTK, Receptor tyrosine kinase; TCM, Traditional Chinese medicine; TCMIs, Traditional Chinese medicine injections; VEGFR, Vascular epithelial growth factor receptor of lead compounds include but are not limited to screening of bioactive compounds with liposomes [4], hollow fiber cell fishing with liquid chromatography (HFCF-HPLC) [5], direct drug screening methods based on target receptors or enzymes [6,7], and virtual screening using computer-assisted drug design (CADD) [8,9]. However, there are few efficient techniques suitable for TCM research.…”