Hollow Polymer Shells from Biological Templates: Fabrication and Potential Applications

Donath, Edwin; Moya, Sergio; Neu, Björn; Sukhorukov, Gleb B.; Georgieva, Radostina; Voigt, Andreas; Bäumler, Hans; Kiesewetter, H.; Möhwald, Helmuth

doi:10.1002/1521-3765(20021202)8:23<5481::aid-chem5481>3.0.co;2-8

Cited by 174 publications

(125 citation statements)

References 11 publications

Supporting

Mentioning

122

Contrasting

Unclassified

Order By: Relevance

“…The technique was usually performed in the aqueous solution at room temperature, and thus, it was suitable for encapsulating therapeutic proteins or other bio-therapeutics with poor stability. Among the decomposable cores porous CaCO 3 drives interest due to its wide industrial, technological, and drug delivery applications (23)(24)(25)(26). The controlled crystallization of CaCO 3 resulting in the formation of uniform, homogenous, and non-aggregated CP.…”

Section: Discussionmentioning

confidence: 99%

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

2011

View full text Add to dashboard Cite

Abstract. The aim of the study was to develop ultrathin polyelectrolyte microreservoir (UPM) using two combinations of synthetic/synthetic (S/s; poly(allylamine hydrochloride) (PAH)/sodium poly (styrenesulfonate)) and synthetic/natural (S/n; PAH/sodium alginate) polyelectrolytes over spherical porous CaCO 3 core particles (CP) followed by core removal and to evaluate its biocompatibility and integrity of loaded model protein bovine serum albumin (BSA). A novel process for synthesis of CP was developed to obtain maximum yield of monodisperse vaterite (spherical) polymorph. The prepared UPM was characterized for surface morphology, layer-by-layer growth, pay load efficiency, integrity of BSA, as well as viability and cell adhesion using murine J 774 macrophages (Φ). In vitro release profile revealed that both S/s and S/n UPM were able to provide sufficient diffusion barrier to release protein at physiological pH. It has been observed that S/n UPM are fully biocompatible due to obvious reason of using natural polymer. In a separate experiment, the S/s UPM surface was modified with pluronic F-68 to tune biocompatibility which provides evidences for safety and tolerability of the S/s UPM as well. In nutshell, the proposed system could successfully be used for the delivery of proteins, and moreover, the system can be tailored to impart desired properties at any stage of layering especially in terms of drug release and to retain the integrity of proteins.

show abstract

Section: Discussionmentioning

confidence: 99%

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

2011

View full text Add to dashboard Cite

show abstract

“…279 A protein destruction treatment was carried out to decompose the cytoplasmic proteins of the erythrocytes, which were removed by centrifugation or filtration. The resulting shells were used for the controlled precipitation or crystallization of organic and inorganic materials.…”

Section: Other Polymeric Systems As Nanoreactorsmentioning

confidence: 99%

Self-Assembled Nanoreactors

et al. 2005

View full text Add to dashboard Cite

“…[1] By self-assembly of oppositely charged polyions, films with precise component locations in the multilayer can be build on charged templates. [2][3][4][5][6][7] So far, a wide variety of inorganic and organic templates for assembly of polyion shells was used like latex, lipid microtubules, blood platelets, erythrocytes, and stem cells, [8][9][10][11][12][13][14][15] and also a number of microbiological materials were applied as a template. In recent studies, for example, Escherichia coli and other bacteria of different genera were encapsulated using electrostatic LbL nano self-assembly and cellular activity LbL nano self-assembly coating of A. vinosum with different polyelectrolyte combinations is presented as an example to investigate substrate uptake in bacteria.…”

Section: Introductionmentioning

confidence: 99%

Layer‐by‐Layer Nano‐Encapsulation of Microbes: Controlled Cell Surface Modification and Investigation of Substrate Uptake in Bacteria

Franz

Balkundi

Dahl

et al. 2010

Macromolecular Bioscience

View full text Add to dashboard Cite

LbL nano self-assembly coating of A. vinosum with different polyelectrolyte combinations is presented as an example to investigate substrate uptake in bacteria. The effects of surface charge and the formation of a physical barrier provides new insights in the contact mechanisms between the cell surface and insoluble elemental sulfur. Furthermore, uptake of sulfide by encapsulated cells was investigated. Growth experiments of coated cells showed that surface charge did neither affect sulfide uptake nor the contact formation between the cells and solid sulfur. However, increasing layers slowed or inhibited the uptake of sulfide and elemental sulfur. This work demonstrates how defining surface properties of bacteria has potential for microbiological and biotechnological applications.

show abstract

Hollow Polymer Shells from Biological Templates: Fabrication and Potential Applications

Abstract: Rhodamin 6G were precipitated within the capsules.

Cited by 174 publications

References 11 publications

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

Templated Ultrathin Polyelectrolyte Microreservoir for Delivery of Bovine Serum Albumin: Fabrication and Performance Evaluation

Self-Assembled Nanoreactors

Layer‐by‐Layer Nano‐Encapsulation of Microbes: Controlled Cell Surface Modification and Investigation of Substrate Uptake in Bacteria

Contact Info

Product

Resources

About