Hollow silica capsules for amphiphilic transport and sustained delivery of antibiotic and anticancer drugs

Gessner, Isabel; Krakor, Eva; Jurewicz, Anna; Wulff, Veronika; Kling, Lasse; Christiansen, Silke; Brodusch, Nicolas; Gauvin, Raynald; Wortmann, Laura; Wolke, Martina; Plum, Georg; Schauß, Astrid; Krautwurst, John; Ruschewitz, Uwe; Ilyas, Shaista; Mathur, Sanjay

doi:10.1039/c8ra03716g

Cited by 17 publications

(20 citation statements)

References 33 publications

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“…[28] To minimize the self-absorption and to have an estimation of the enzyme concentration, the iron oxide core (α-Fe 2 O 3 ) was etched using hydrochloric acid revealing hollow mesoporous silica capsules (HMSC). [21] The HMSC was subsequently modified with both enzymes, and a Bradford assay was performed, which revealed a concentration of 57.6 µg enzyme in 1 mg HMSC-cutinase and 49.8 µg enzyme for the HMSC-lipase. However, given the higher density of magnetite, absolute numbers cannot be used for a quantitative comparison and are expected to be slightly lower for For each modification step, the at.% of C, Si, O, (Fe), and N was found to vary with the attachment of functional groups to the particle surface resulting in thicker films.…”

Section: Resultsmentioning

confidence: 99%

“…This was a two-step synthesis in which first, α-Fe 2 O 3 @SiO 2 particles were synthesized as previously described by Gessner and Krakor et al [21] In a consequent step, the α-Fe 2 O 3 core was reduced under H 2 /Ar atmosphere at 400°C for 12 h with a heating rate of 10°C/min to obtain Fe 3 O 4 nanoparticles, while preserving the shape.…”

Section: Synthesis Of Fe 3 O 4 @Sio 2 Particlesmentioning

confidence: 99%

“…To determine the enzyme concentration for Fe 3 O 4 @ SiO 2 particles, the iron core was etched using HCl, as described in our previous publication. [21] The hollow mesoporous silica capsules (HMSC) obtained were modified with enzymes as described above, and the enzyme concentration was quantified using the Bradford assay.…”

Section: Protein Quantification Using Bradford Assaymentioning

confidence: 99%

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Selective degradation of synthetic polymers through enzymes immobilized on nanocarriers

et al. 2021

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In order to develop new sustainable and reusable concepts for the degradation of omnipresent industrial plastics, immobilization of (bio)catalysts on nanocarriers offers unique opportunities for selective depolymerization and catalyst recovery. In this study, enzymes (lipase and cutinase) were covalently immobilized on carrier nanoparticles (SiO2 and Fe3O4@SiO2) through 3-(aminopropyl)trimethoxysilane and glutaraldehyde linkers forming a stable bond to enzyme molecules. The presence of enzymes on the surface was confirmed by zeta potential and XPS measurements, while their degradation activity and long-term stability of up to 144 h was demonstrated by the conversion of 4-nitrophenyl acetate to 4-nitrophenol. Furthermore, enzymatic decomposition (hydrolysis/oxidation) of electrospun polycaprolactone fiber mats was verified through morphological (SEM) and weight loss studies, which evidently showed a change in the fiber morphology due to enzymatic degradation and accordingly a weight loss. Graphic abstract

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Section: Resultsmentioning

confidence: 99%

Section: Synthesis Of Fe 3 O 4 @Sio 2 Particlesmentioning

confidence: 99%

Section: Protein Quantification Using Bradford Assaymentioning

confidence: 99%

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Selective degradation of synthetic polymers through enzymes immobilized on nanocarriers

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“…Synthesis of ellipsoid-shaped HMSCs. The synthesis of ellipsoid-shaped hollow mesoporous silica capsules (HMSCs) was performed as previously described 42 using ellipsoid-shaped hematite particles as hard templates. In short, hematite (Fe 2 O 3 ) particles, produced in a solvothermal process, 43 were subsequently coated in a two-step sol gel process with silica (SiO 2 ) to form core-shell (Fe 2 O 3 @SiO 2 ) particles.…”

Section: Accepted Articlementioning

confidence: 99%

“…26 As an alternative strategy, we report on the immobilization of copper, silver, and zinc containing nanoparticles on hollow mesoporous silica capsules (HMSCs), which have been previously reported as biodegradable and amphiphilic drug delivery vehicles that can efficiently transport high payloads of both hydrophilic and hydrophobic drugs. 42 In addition to their surface-immobilization on silica capsules, metalbased nanoparticles were synthesized inside and on the outside of HMSCs through in-situ reduction of appropriate metal salts to obtain Ag@HMSCs, Cu@HMSCs, and ZnO@HMSCs following a "ship-in-abottle" reaction. The mesoporous nature of the walls of silica capsule allowed a slow outward diffusion of metal ions leaching from the inner core that prolonged the stream of metal ions following the burst release triggered by surface-anchored nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

Hollow mesoporous silica capsules loaded with copper, silver, and zinc oxide nanoclusters for sustained antibacterial efficacy

et al. 2021

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Intensive and overuse of antibiotics during the last years has triggered a distinct rise in antibiotic resistance worldwide. In addition to the newly developed antimicrobials, there is a high demand for alternative treatment options against persistent bacterial infections. The biocidal impact of transition metal ions like copper (Cu 2+ ), silver (Ag + ), and zinc (Zn 2+ ) also known as oligodynamic effect has been used through ages to kill or inhibit the growth of microorganisms and to employ long-term prevention strategies against their biological antagonists. Herein, we report on the synthesis of Cu, Ag and Zn metal Accepted ArticleThis article is protected by copyright. All rights reserved and corresponding oxide nanoparticles immobilized on hollow mesoporous silica capsules (HMSCs) obtained by a hard-template assisted sol-gel synthesis followed by reduction of appropriate metal salts in the presence of HMSCs. Compartmentalization of nanosized metal and oxide clusters in Ag@HMSCs, Cu@HMSCs and ZnO@HMSCs particles prevented their agglomeration and offered a high release kinetics of metal ions between 2.0-3.7 mM during 24 hours, as monitored by UV-vis analyses. The distribution and morphology of pristine and metal functionalized HMSCs was evaluated by transmission electron microscopy (TEM) analysis revealing the successful synthesis of Ag, Cu and ZnO nanoparticles supported on HMSCs. X-ray photoelectron spectroscopy (XPS) revealed that mainly Cu(II), Ag(0) and Zn(II) species were present in the modified HMSCs. In addition to the surface attachment of preformed metal (Ag and Cu) and metal oxide (ZnO) cluster, nucleation of metal nanoparticles inside the void of HMSCs provided an internal reservoir which allowed for a time-dependent release of metal ions through slower dissolution rates leading to a long-term and sustained bacterial inhibition over several hours. The high antimicrobial efficiency of Ag@HMSCs, Cu@HMSCs, and ZnO@HMSCs particles was investigated towards both Grampositive (B. subtilis) and Gram-negative (E. coli) bacteria by INT assays showing a complete growth inhibition for both bacteria types after 24 hours. While Ag@HMSCs and Cu@HMSCs showed a higher susceptibility against Gram-negative bacteria, ZnO@HMSCs showed a higher susceptibility against Grampositive bacteria. This demonstrates the promise of metal-loaded capsules as antibacterial delivery vehicles with dual-mode time release profiles being potential alternatives for antibiotic drugs.

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Zwitterionic Polymer Electroplating Facilitates the Preparation of Electrode Surfaces for Biosensing

et al. 2022

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Surface chemistry critically affects the diagnostic performance of biosensors. An ideal sensor surface should be resistant to nonspecific protein adsorption, yet be conducive to analytical responses. Here a new polymeric material, zwitterionic polypyrrole (ZiPPy), is reported to produce optimal surface condition for biosensing electrodes. ZiPPy combines two unique advantages: the zwitterionic function that efficiently hydrates electrode surface, hindering nonspecific binding of hydrophobic proteins; and the pyrrole backbone, which enables rapid (<7 min), controlled deposition of ZiPPy through electropolymerization. ZiPPy‐coated electrodes show lower electrochemical impedance and less nonspecific protein adsorption (low fouling), outperforming bare and polypyrrole‐coated electrodes. Moreover, affinity ligands for target biomarkers can be immobilized together with ZiPPy in a single‐step electropolymerization. ZiPPy‐coated electrodes are developed with specificity for severe acute respiratory syndrome coronavirus 2 (SARS‐CoV‐2). The prepared sensor detects SARS‐CoV‐2 antibodies in human saliva down to 50 ng mL−1, without the need for sample purification or secondary labeling.

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Hollow silica capsules for amphiphilic transport and sustained delivery of antibiotic and anticancer drugs

Abstract: Hollow mesoporous silica capsules were used as amphiphilic drug delivery vehicles and sustained release systems for antimicrobial and anticancer drugs.

Cited by 17 publications

References 33 publications

Selective degradation of synthetic polymers through enzymes immobilized on nanocarriers

Selective degradation of synthetic polymers through enzymes immobilized on nanocarriers

Hollow mesoporous silica capsules loaded with copper, silver, and zinc oxide nanoclusters for sustained antibacterial efficacy

Zwitterionic Polymer Electroplating Facilitates the Preparation of Electrode Surfaces for Biosensing

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