Hollow spherical nucleic acid structures based on polymer-coated phospholipid vesicles

Haladjova, Emi; Petrova, Maria; Ugrinova, Iva; Foryś, Aleksander; Trzebicka, Barbara; Rangelov, Stanislav

doi:10.1039/d2sm00355d

Cited by 2 publications

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“…SNAs have been prepared with a variety of core materials including inorganic compositions (Au, Ag, Pt, SiO 2 , Fe 3 O 4 , quantum dots) as well as organic and polymeric compositions. ,− Although introducing great versatility in the chemistry, structure, and properties of the SNAs, the latter are yet underexplored. They can be obtained by self-assembly of amphiphilic conjugates, prepared from a synthetic polymer covalently linked to a short oligonucleotide strand, into core–shell type micelles.…”

Section: Introductionmentioning

confidence: 99%

“…They can be obtained by self-assembly of amphiphilic conjugates, prepared from a synthetic polymer covalently linked to a short oligonucleotide strand, into core–shell type micelles. Normally, the synthetic polymers are hydrophobic – polyesters such as poly(lactic- co -glycolic acid) and poly(ε-caprolactone), ,− polyethers such as poly(ethoxyethyl glycidyl ether), and also diene and vinyl polymers such as polybutadiene and polystyrene, respectively – and build the core of the structures, whereas the nucleic acid strands form the shell. Most of the polymer-oligonucleotide conjugates are of linear diblock and rarely of triblock, chain architecture, which poses some limitations to obtain structures with high enough density of nucleic acids on the nanoparticle surface, capable of exhibiting the typical/hallmark properties of SNAs.…”

Section: Introductionmentioning

confidence: 99%

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Three-Dimensional Nucleic Acid Nanostructures Based on Self-Assembled Polymer-Oligonucleotide Conjugates of Comblike and Coil-Comb Chain Architectures

et al. 2023

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Spherical nucleic acids have emerged as a class of nanostructures, exhibiting a wide variety of properties, distinctly different from those of linear nucleic acids, and a plethora of applications in therapeutics and diagnostics. Herein, we report on preparation of 3D nucleic acid nanostructures, prepared by self-assembly of polymer–oligonucleotide conjugates. The latter are obtained by grafting multiple alkyne-functionalized oligonucleotide strands onto azide-modified homo-, block, and random (co)polymers of chloromethylstyrene via initiator-free click coupling chemistry to form conjugates of comblike and coil-comb chain architectures. The resulting conjugates are amphiphilic and form stable nanosized supramolecular structures in aqueous solution. The nanoconstructs are thoroughly investigated and a number of physical characteristics, in particular, molar mass, size, aggregation number, zeta potential, material density, number of oligonucleotide strands per particle, grafting density, and their relation to hallmark properties of spherical nucleic acids – biocompatibility, resistance against DNase I, cellular uptake without the need for transfection agents – are determined.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Nucleic Acid Nanostructures Based on Self-Assembled Polymer-Oligonucleotide Conjugates of Comblike and Coil-Comb Chain Architectures

et al. 2023

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From Structure to Application: The Evolutionary Trajectory of Spherical Nucleic Acids

Wang,

Han,

2024

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Since the proposal of the concept of spherical nucleic acids (SNAs) in 1996, numerous studies have focused on this topic and have achieved great advances. As a new delivery system for nucleic acids, SNAs have advantages over conventional deoxyribonucleic acid (DNA) nanostructures, including independence from transfection reagents, tolerance to nucleases, and lower immune reactions. The flexible structure of SNAs proves that various inorganic or organic materials can be used as the core, and different types of nucleic acids can be conjugated to realize diverse functions and achieve surprising and exciting outcomes. The special DNA nanostructures have been employed for immunomodulation, gene regulation, drug delivery, biosensing, and bioimaging. Despite the lack of rational design strategies, potential cytotoxicity, and structural defects of this technology, various successful examples demonstrate the bright and convincing future of SNAs in fields such as new materials, clinical practice, and pharmacy.

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Hollow spherical nucleic acid structures based on polymer-coated phospholipid vesicles

Abstract: A feasible one pot synthesis of hollow spherical nucleic acids (SNAs) using phospholipid liposomes is reported. These constructs are synthesized in a chemically straightforward process involving formation of unilamellar liposomes,...

Cited by 2 publications

References 56 publications

Three-Dimensional Nucleic Acid Nanostructures Based on Self-Assembled Polymer-Oligonucleotide Conjugates of Comblike and Coil-Comb Chain Architectures

Three-Dimensional Nucleic Acid Nanostructures Based on Self-Assembled Polymer-Oligonucleotide Conjugates of Comblike and Coil-Comb Chain Architectures

From Structure to Application: The Evolutionary Trajectory of Spherical Nucleic Acids

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