Holly (Ilex latifolia Thunb.) Polyphenols Extracts Alleviate Hepatic Damage by Regulating Ferroptosis Following Diquat Challenge in a Piglet Model

He, Pengwei; Hua, Hongwei; Tian, Wei; Zhu, Huiling; Liu, Yulan

doi:10.3389/fnut.2020.604328

Cited by 18 publications

(12 citation statements)

References 37 publications

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“…Earlier work has demonstrated that iron, a major component of haemoglobin, which is extremely toxic to nerve cells, is liberated during haematoma formation following ICH and can initiate a Fenton reaction. The latter leads to the generation of multiple hydroxyl radicals, which stimulate and exacerbate tissue oxidation which harms cellular membranes, DNA and proteins and gives rise to nerve cell ferroptosis (Li et al 2017a, b, c;He et al 2020;Magtanong and Dixon 2018;Zhang et al 2018a, b;Djulbegovic and Uversky 2019).…”

Section: Discussionmentioning

confidence: 99%

Scalp Acupuncture Protects Against Neuronal Ferroptosis by Activating The p62-Keap1-Nrf2 Pathway in Rat Models of Intracranial Haemorrhage

Dai

et al. 2021

J Mol Neurosci

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Intracerebral haemorrhage (ICH) can be a catastrophic event; even if the initial stages of the pathology were well-managed, a number of patients experience varied residual neurological deficits following the insult. Ferroptosis is a recently identified type of cell demise which is tightly linked to the neurological impairment associated with ICH. In the current work, the prophylactic impact of scalp acupuncture (SA) therapy on autologous blood injection murine models of ICH was investigated in order to establish whether SA could mitigate the secondary damage arising following ICH by moderating ferroptosis. The pathophysiological mechanisms associated with this process were also explored. Ludmila Belayev tests were utilised for the characterisation of neurological damage. Haematoxylin–eosin staining was employed in order to determine the cerebral impact of the induced ICH. Malondialdehyde (MDA) and iron titres in peri-haemorrhagic cerebral tissues were appraised using purchased assay kits. Transmission electron microscopy delineated mitochondrial appearances within nerve cell bodies from the area of haemorrhage. Western blotting techniques were utilised to assay the degree of protein expression of NeuN, sequestosome 1 (p62), nuclear factor erythroid 2-related factor 2 (Nrf2), Kelch-like ECH-associated protein 1 (Keap1), glutathione peroxidase 4 (GPX4) and ferritin heavy chain 1 (FTH1). The frequencies of Nrf2, GPX4 and FTH1 positive cells, respectively, were documented with immunohistochemical staining. The results demonstrated that therapy with SA after ICH mitigated MDA and iron sequestration, diminished the appearance of contracted mitochondria with increased outer mitochondrial membrane diameter within the nerve cell bodies, and suppressed neuronal ferroptosis. The pathways responsible for these effects may encompass amplified p62, Nrf2, GPX4 and FTH1 expression, together with decreased Keap1 expression. Application of SA reduced identified neurobehavioural abnormalities after ICH; no disparities were observed between the consequences of SA therapy and deferoxamine delivery. It can be surmised that intervention with SA enhanced recovery after ICH by triggering the antioxidant pathway, p62/Keap1/Nrf2, and causing FTH1 and GPX4 upregulation, factors that participate in diminishing excess iron and thus in mitigating lipid peroxidation insults arising from ferroptosis following ICH.

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Section: Discussionmentioning

confidence: 99%

Scalp Acupuncture Protects Against Neuronal Ferroptosis by Activating The p62-Keap1-Nrf2 Pathway in Rat Models of Intracranial Haemorrhage

Dai

et al. 2021

J Mol Neurosci

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“…The hepatocytes ultrastructure were observed and photographed with a HT7700 TEM (Hitachi Co. Ltd., Tokyo, Japan) referred to previous study [ 26 ].…”

Section: Methodsmentioning

confidence: 99%

Glycine alleviated diquat-induced hepatic injury via inhibiting ferroptosis in weaned piglets

Hua

Tian

et al. 2022

Anim Biosci

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Objective:The beneficial effects of glycine were tested in piglets with diquat-induced hepatic injury.Methods: Thirty-two piglets were assigned by a 2 × 2 factorial experimental design including glycine supplementation and diquat challenge. After 3 weeks of feeding with a basic diet or a 1% glycine supplemented diet, piglets were challenged with diquat or saline. After 1 week later, the piglets were slaughtered and samples were collected. Results:Our results indicated that glycine alleviated diquat induced morphological hepatic injury, decreased the activities of plasma alanine aminotransferase, aspartate aminotransferase and glutamyl transpeptidase in the piglets under diquat challenge, and increased total antioxidantive capacity and antioxidative enzyme activity significantly. Adding with glycine enhanced the concentrations of hepatic adenosine triphosphate and adenosine diphosphate. Transmission electron microscope observation showed that diquat induced clear hepatocytes ferroptosis and its effect could be alleviated by glycine to a certain degree. Moreover, glycine significantly affected mRNA and protein expression of ferroptosis-related signals in the liver. Conclusion:These results demonstrated that glycine attenuated liver damage via inhibiting ferroptosis.

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“…Other polyphenol-containing food additives have been assessed for their ability to reduce oxidative stress and inflammation. For example, holly tree derived polyphenol extracts were associated with improved liver function in weaned piglets challenged with oxidative stress using diquat, as evident from heightened anti-oxidant capacity, and expression of metabolic transferases and ferroptosis mediators in the liver [ 228 ]. Resveratrol (a stilbene common to berries) added to diets reduced Salmonella and E. coli counts in the fecal content of pathogen challenged weaned piglets, additionally increasing serum immunoglobin G (IgG), reducing TNF-α levels, and appearing to improve the gut microenvironment in general due to improvements in nitrogen digestibility [ 229 ].…”

Section: Health Effects Of Dietary Polyphenols In Pigs and Other Animalsmentioning

confidence: 99%

Polyphenols: Bioavailability, Microbiome Interactions and Cellular Effects on Health in Humans and Animals

2022

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Polyphenolic compounds have a variety of functions in plants including protecting them from a range of abiotic and biotic stresses such as pathogenic infections, ionising radiation and as signalling molecules. They are common constituents of human and animal diets, undergoing extensive metabolism by gut microbiota in many cases prior to entering circulation. They are linked to a range of positive health effects, including anti-oxidant, anti-inflammatory, antibiotic and disease-specific activities but the relationships between polyphenol bio-transformation products and their interactions in vivo are less well understood. Here we review the state of knowledge in this area, specifically what happens to dietary polyphenols after ingestion and how this is linked to health effects in humans and animals; paying particular attention to farm animals and pigs. We focus on the chemical transformation of polyphenols after ingestion, through microbial transformation, conjugation, absorption, entry into circulation and uptake by cells and tissues, focusing on recent findings in relation to bone. We review what is known about how these processes affect polyphenol bioactivity, highlighting gaps in knowledge. The implications of extending the use of polyphenols to treat specific pathogenic infections and other illnesses is explored.

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Holly (Ilex latifolia Thunb.) Polyphenols Extracts Alleviate Hepatic Damage by Regulating Ferroptosis Following Diquat Challenge in a Piglet Model

Cited by 18 publications

References 37 publications

Scalp Acupuncture Protects Against Neuronal Ferroptosis by Activating The p62-Keap1-Nrf2 Pathway in Rat Models of Intracranial Haemorrhage

Scalp Acupuncture Protects Against Neuronal Ferroptosis by Activating The p62-Keap1-Nrf2 Pathway in Rat Models of Intracranial Haemorrhage

Glycine alleviated diquat-induced hepatic injury via inhibiting ferroptosis in weaned piglets

Polyphenols: Bioavailability, Microbiome Interactions and Cellular Effects on Health in Humans and Animals

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