Holm Oak Quercus ilex L.

Corredoira, Elena; Hernández, Inmaculada; Morcillo, Marian; Martínez, M. T.; Ruiz-Galea, Mar; Cernadas, Ma José; Ramírez-Martín, Noelia; San-José, M. C.; Arrillaga, Isabel; Toribio, M.

doi:10.1007/978-3-319-89483-6_14

Cited by 1 publication

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“…Indeed, somatic embryos or/and embryogenic callus generated on the initial explants were isolated and cultured in proliferation medium to produce numerous somatic embryos by secondary embryogenesis. However, the maintenance of embryogenic ability in holm oak is one the main bottlenecks as most of the initial embryogenic cultures lost their embryogenic competence after few subcultures (Blasco et al., 2013; Barra-Jiménez et al., 2014; Martínez et al., 2017b; Corredoira et al., 2018b). Furthermore, after isolation, some primary embryos underwent rapid differentiation to the cotyledonary stage, sometimes even forming complete plants without developing secondary somatic embryos.…”

Section: Holm Oak Somatic Embryogenesis: Where Do We Stand At Present?mentioning

confidence: 99%

“…Sánchez et al., 2003; Hernández et al., 2003b; Andrade and Merkle, 2005; Corredoira et al., 2008). Nevertheless, the genotype effect is less in holm oak than in other oak species studied and plantlet conversion rates have improved after optimization of proliferation and maintenance of embryogenic lines (Corredoira et al., 2018b). In addition, good quality of plants in terms of shoot length and leaf development is observed in holm oak (Figures 2D,E).…”

Section: Holm Oak Somatic Embryogenesis: Where Do We Stand At Present?mentioning

confidence: 99%

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Holm Oak Somatic Embryogenesis: Current Status and Future Perspectives

et al. 2019

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Quercus ilex (holm oak) is one of the most representative trees in the Mediterranean basin, but now the sustainability of its ecosystems is at serious risk due to the lack of natural regeneration and to the presence of a severe disease called oak decline that has caused the death of thousands of trees. The application of biotechnological tools, such as somatic embryogenesis, allows programs of genetic improvement of the species to be speeded up and helps in the conservation of its ecosystems. Somatic embryogenesis is currently considered one of the main biotechnological techniques that has demonstrated significant benefits when has applied to forest tree species, providing significant advantages such as mass propagation, genetic transformation, application of synthetic seed, and cryopreservation of elite genotypes. In this report, the state of the art of somatic embryogenesis in holm oak is reviewed. Factors affecting the induction (plant donor age, type of explant, or plant growth regulators) and maintenance and proliferation of the embryogenic cultures are summarized. Advances on the conversion of somatic embryos into plants and on the acclimatization of these plantlets, as well as the results obtained on the application of the genetic transformation and the cryopreservation procedures to holm oak embryogenic cultures, are also presented.

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