CO 2 emissions from preindustrial land-use change (LUC) are subject to large uncertainties. Although atmospheric CO 2 records suggest only a small land carbon (C) source since 5,000 y before present (5 kyBP), the concurrent C sink by peat buildup could mask large early LUC emissions. Here, we combine updated continuous peat C reconstructions with the land C balance inferred from double deconvolution analyses of atmospheric CO 2 and δ 13 C at different temporal scales to investigate the terrestrial C budget of the Holocene and the last millennium and constrain LUC emissions. LUC emissions are estimated with transient model simulations for diverging published scenarios of LU area change and shifting cultivation. Our results reveal a large terrestrial nonpeatland C source after the Mid-Holocene (66 ± 25 PgC at 7-5 kyBP and 115 ± 27 PgC at 5-3 kyBP). Despite high simulated per-capita CO 2 emissions from LUC in early phases of agricultural development, humans emerge as a driver with dominant global C cycle impacts only in the most recent three millennia. Sole anthropogenic causes for particular variations in the CO 2 record (∼20 ppm rise after 7 kyBP and ∼10 ppm fall between 1500 CE and 1600 CE) are not supported. This analysis puts a strong constraint on preindustrial vs. industrial-era LUC emissions and suggests that upper-end scenarios for the extent of agricultural expansion before 1850 CE are not compatible with the C budget thereafter.carbon cycle | Anthropocene | agriculture | peatland | ice core T he Earth's functioning is now so significantly affected by human activities that their impacts will leave long-lasting fingerprints in environmental records. This has motivated the definition of a new, human-dominated geological epoch-the Anthropocene (1, 2). However, human impacts on the Earth's environmental history throughout the preindustrial Holocene are less clear. Although a causal link between anthropogenic greenhouse gas (GHG) emissions and the rapid rise in their concentrations since industrialization is unequivocal, CO 2 and CH 4 concentrations already exhibited a particular, albeit slow and not synchronous increase millennia earlier. This may give rise to the possibility of far-reaching global environmental change induced by early agriculture (3). Similarly, variations in atmospheric CO 2 during the last millennium have been linked to agricultural expansion and collapse (2). However, any association between a particular change in the GHG records and a coincidental continental or global socio-economic change hinges on a causal relationship between the two and on the plausibility of bottom-up estimates to imply a sufficient impact on atmospheric GHG concentrations.In all cases where such a connection has been claimed, causality is strongly debated (4-8). For example, the link between the particular CO 2 increase after 7 kyBP and the Neolithic Revolution is motivated by a multitude of local-scale paleoecological and archaeological archives documenting an early onset of a human influence on the landscape wit...