Rock varnish, a dark-coloured natural feature rich in manganese (Mn), iron (Fe), and clay minerals, is believed to provide nutritional support to microbiota. Thus, rock varnish is considered a unique substrate for potential microbial life to thrive in the extreme environments on Earth that are comparable to their planetary analogues. However, little is known about the occurrence of microbiota in rock varnish, as the microbes found on the varnish are quite diversified. We present here the new morphological and chemical results of microbial forms found in rock varnish samples from Ladakh, a potential site for hosting life in extreme environments. Our results demonstrate the presence of putative magnetofossils type biological entities in the form of nanochains present in the rock varnish layer that coincide with high magnetic susceptibility values of varnish samples. Further, the higher concentrations of oxidised fractions of Mn 4+ , and carboxylic acid functionality on the varnish surface revealed the signatures of organic entities. These collective results point towards the enriched concentration of magnetic minerals on the varnish layer that are possibly sourced through biotic forms. Consequently, the rock varnish can serve as a "black box" of ancient environmental records, as well as a potential geomaterial for astrobiological studies from the Martian analogue field location of Ladakh, which needs to be explored further for extensive biogeochemical studies.