Holographic twin traps

Zwick, Susanne; Haist, Tobias; Miyamoto, Yoko; He, Lin; Warber, Michael; Hermerschmidt, Andreas

doi:10.1088/1464-4258/11/3/034011

Cited by 40 publications

(37 citation statements)

References 44 publications

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“…Counterpropagating diverging beams can also be used to trap objects by using opposing objective lenses 39,40 or using a dichroic sample slide or mirror behind the sample. 41,42 The scattering forces from a pair of beams cancel when the object is centered axially, removing the requirement for high NA. This enables the use of long working distance objectives at low magnifications.…”

Section: Trapping Capabilitiesmentioning

confidence: 99%

A compact holographic optical tweezers instrument

Gibson

Bowman

Linnenberger

et al. 2012

Review of Scientific Instruments

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Visual and dynamical measurement of Rayleigh-Benard convection by using fiber-based digital holographic interferometry J. Appl. Phys. 112, 113113 (2012) Guide-star-based computational adaptive optics for broadband interferometric tomography Appl. Phys. Lett. 101, 221117 (2012) Limits of elemental contrast by low energy electron point source holography J. Appl. Phys. 110, 094305 (2011) Cantilever biosensor reader using a common-path, holographic optical interferometer Appl. Phys. Lett. 97, 221110 (2010) Extended depth of focus in a particle field measurement using a single-shot digital hologram Appl. Phys. Lett. 95, 201103 (2009) Additional information on Rev. Sci. Instrum. Holographic optical tweezers have found many applications including the construction of complex micron-scale 3D structures and the control of tools and probes for position, force, and viscosity measurement. We have developed a compact, stable, holographic optical tweezers instrument which can be easily transported and is compatible with a wide range of microscopy techniques, making it a valuable tool for collaborative research. The instrument measures approximately 30×30×35 cm and is designed around a custom inverted microscope, incorporating a fibre laser operating at 1070 nm. We designed the control software to be easily accessible for the non-specialist, and have further improved its ease of use with a multi-touch iPad interface. A high-speed camera allows multiple trapped objects to be tracked simultaneously. We demonstrate that the compact instrument is stable to 0.5 nm for a 10 s measurement time by plotting the Allan variance of the measured position of a trapped 2 μm silica bead. We also present a range of objects that have been successfully manipulated.

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Section: Trapping Capabilitiesmentioning

confidence: 99%

A compact holographic optical tweezers instrument

Gibson

Bowman

Linnenberger

et al. 2012

Review of Scientific Instruments

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show abstract

“…The need for a high numerical aperture for stable axial trapping has confined HOT to situations where samples could be accessed using short working distance objectives. Using a mirror trap configuration [9,10] and a diamond anvil cell (DAC) [11] we have recently shown that the full power of holographic tweezers can be also made available for high pressure studies [12,13]. Optical tweezers have been successfully applied to the study of the mechanical and rheological response of soft and biological matter [14].…”

Section: Introductionmentioning

confidence: 99%

Holographic tracking and sizing of optically trapped microprobes in diamond anvil cells

et al. 2016

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We demonstrate that Digital Holographic Microscopy can be used for accurate 3D tracking and sizing of a colloidal probe trapped in a diamond anvil cell (DAC). Polystyrene beads were optically trapped in water up to Gigapascal pressures while simultaneously recording in-line holograms at 1 KHz frame rate. Using Lorenz-Mie scattering theory to fit interference patterns, we detected a 10% shrinking in the bead's radius due to the high applied pressure. Accurate bead sizing is crucial for obtaining reliable viscosity measurements and provides a convenient optical tool for the determination of the bulk modulus of probe material. Our technique may provide a new method for pressure measurements inside a DAC.

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“…Indeed, two fibers with numerical apertures as low as 0.1 are able to provide sufficient forces for trapping with a further benefit of requiring a low power density, thus reducing potential photodamage [12,13]. This configuration can also be achieved by creating two foci along the propagation axis from a single beam and reflecting the beam with a mirror to create a counterpropagating configuration [14,15]. Notably, with this arrangement, micro-organisms with sizes ranging from 50 µm to 100 µm have been successfully trapped [16].…”

Section: Introductionmentioning

confidence: 99%

Macro-optical trapping for sample confinement in light sheet microscopy

Yang

Piksarv

Ferrier

et al. 2015

Biomed. Opt. Express

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Abstract:Light sheet microscopy is a powerful approach to construct three-dimensional images of large specimens with minimal photo-damage and photo-bleaching. To date, the specimens are usually mounted in agents such as agarose, potentially restricting the development of live samples, and also highly mobile specimens need to be anaesthetized before imaging. To overcome these problems, here we demonstrate an integrated light sheet microscope which solely uses optical forces to trap and hold the sample using a counter-propagating laser beam geometry. Specifically, tobacco plant cells and living Spirobranchus lamarcki larvae were successfully trapped and sectional images acquired. This novel approach has the potential to significantly expand the range of applications for light sheet imaging.

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Holographic twin traps

Cited by 40 publications

References 44 publications

A compact holographic optical tweezers instrument

A compact holographic optical tweezers instrument

Holographic tracking and sizing of optically trapped microprobes in diamond anvil cells

Macro-optical trapping for sample confinement in light sheet microscopy

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