Introduction.Let W be a domain in C or a Riemann surface and let S be a Riemann surface of type (g, n) with Sg -3 + n > 0, where g is the genus of S and n is the number of punctures of S. In this paper, we shall consider holomorphic families of S over W (see Section 1, Definition 1) or a locally holomorphic mapping of W to the Teichmϋller space of S, and study their boundary behavior.In Section 1, we shall state known results and set up our notations.In Section 2, we shall investigate a holomorphic family of S over the punctured disk 0 < \z\ < 1 and the behavior as z->0. Imayoshi [7] obtained a similar result. We shall show a uniqueness theorem of holomorphic families of S (Theorem 2).In Section 3, we shall discuss holomorphic families over a general domain or a Riemann surface, and consider the problems as in Section 2.In Section 4, we construct two examples of holomorphic families which might be of interest.Thanks are due to Professors Y. Imayoshi and M. Taniguchi for useful and stimulating conversation. The author expresses his thanks also to the referee for valuable suggestions.