Homing of chloromethylbenzoyl ammonia-labeled bone marrow mesenchymal stem cells in an immune-mediated bone marrow failure mouse model in vivo

Abstract

ABSTRACT. Aplastic anemia is an abnormal immune reaction disease in which T lymphocytes destroy hematopoietic stem and progenitor cells because of immune hyperactivity. Bone marrow mesenchymal stem cells (BMSCs) have hematopoietic supporting and immune regulation functions. This study investigated BMSCs homing in mice transplantation models after bone marrow failure. BALB/c mice were randomly divided into three groups: normal control, bone marrow failure model, and BMSC transplantation group. Chloromethyl benzamidolabeled BMSCs of BALB/c mice were transplanted through tail vein injection in mouse models with bone marrow failure. Flow cytometry and histological fluorescence microscopy were used to observe the dynamic distribution of labeled cells in different tissues. Average survival time, peripheral blood, and bone marrow morphological features were observed in mice from each group. Twenty-four hours after tail vein infusion of BMSCs, positively labeled cells were observed in the bone marrows of recipient mice, and the number of positive cells increased significantly at 72 h (P < 0.05). In dead or dying mice, white blood cells, hemoglobin, platelets, and bone marrow mononuclear cells were all significantly higher in the BMSC transplantation group than in the BMSCs of the model group (P < 0.01). Mean survival time was significantly shorter in the bone marrow failure model group than in the transplantation group (P < 0.05). These results confirmed that the major of BMSCs injected via tail vein could migrate to injured bone marrow tissues within 24-72 h in a mouse model of bone marrow failure. Furthermore, BMSCs can promote hematopoietic recovery, reduce the degree of bone marrow failure, and significantly prolong survival time.

Exogenous IL-4-Expressing Bone Marrow Mesenchymal Stem Cells for the Treatment of Autoimmune Sensorineural Hearing Loss in a Guinea Pig Model

“…Bone marrow mesenchymal stem cells (BMSCs) are considered favorable vehicles for gene therapy owing to their multidifferentiation potential and their ability to migrate into sites of acute injury [2–7]. In vitro experiments have demonstrated long-term expression of exogenous genes delivered into BMSCs, thus making it possible to use genetically modified BMSCs for the treatment of diseases [8].…”

“…In such cases, the amount of lentiviral vectors needed for the treatment is significantly lower, thus lowering the risk of adverse events. Numerous reports have demonstrated the therapeutic value of BMSCs [2, 7–11]. Furthermore, BMSCs play immunoregulatory roles and are involved in the genesis and progression of autoimmune diseases [12].…”

Comparison of Three Types of Mesenchymal Stem Cells (Bone Marrow, Adipose Tissue, and Umbilical Cord-Derived) as Potential Sources for Inner Ear Regeneration

“…In this regard, various aspects of developmental biology and molecular signaling networks must be considered 68,69 . Multi differentiation potential of MSCs and their capability to migrate into acute injury location make these cells suitable candidate for gene and cell therapy [70][71][72][73][74] . MSCs have been demonstrated to be helpful in treating inner ear inflammatory damage because they exhibit multidirectional differentiation potential, immunosuppressive function and low immunogenicity 75 .…”

Epimedium Polysaccharide Ameliorates Benzene‐Induced Aplastic Anemia in Mice

“…e BIAA mouse model was established according to the accepted criteria and evaluated by body weight, peripheral blood cell counts, peripheral blood cell smears, and femur biopsies [27]. e body weights of all mice were measured once a week using an automatic balance.…”