2008
DOI: 10.1016/j.stem.2008.08.002
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Homing of Mouse Spermatogonial Stem Cells to Germline Niche Depends on β1-Integrin

Abstract: Spermatogonial stem cells (SSCs) provide the foundation for spermatogenesis. In a manner comparable to hematopoietic stem cell transplantation, SSCs colonize the niche of recipient testes and reinitiate spermatogenesis following microinjection into the seminiferous tubules. However, little is known about the homing mechanism of SSCs. Here we examined the role of adhesion molecules in SSC homing. SSCs isolated from mice carrying loxP-tagged beta1-integrin alleles were ablated for beta1-integrin expression by in… Show more

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Cited by 166 publications
(121 citation statements)
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“…Vimentin, an intermediate filament of Sertoli cell cytoskeleton, plays an important role in maintenance of spermatogenesis in the testes [53]. Using qPCR, we further confirmed the expression of mRNA encoding other SSC specific markers (viz., β1 integrin and Oct-4) required for colonization and self renewal of SSCs [54,55]. A relatively higher abundance of vimentin mRNA observed in the study might be due to the fact that most SSC cultures contain a mixture of testicular cells constituting as less as 1.33 % SSCs and other testicular cells mainly comprising of Sertoli cells [26].…”
Section: Discussionmentioning
confidence: 71%
“…Vimentin, an intermediate filament of Sertoli cell cytoskeleton, plays an important role in maintenance of spermatogenesis in the testes [53]. Using qPCR, we further confirmed the expression of mRNA encoding other SSC specific markers (viz., β1 integrin and Oct-4) required for colonization and self renewal of SSCs [54,55]. A relatively higher abundance of vimentin mRNA observed in the study might be due to the fact that most SSC cultures contain a mixture of testicular cells constituting as less as 1.33 % SSCs and other testicular cells mainly comprising of Sertoli cells [26].…”
Section: Discussionmentioning
confidence: 71%
“…A new discovery in the present study is the homing and colonization process of GS cells, which were promising signs of successful sperm production in vitro, because the differentiation of SSCs on the basement membrane was already demonstrated in our previous study 15 . The mechanism of SSC migration from the lumen to the basal compartment of the seminiferous tubule is not fully understood 17 . The organ culture system would be useful to elucidate SSCs' behaviour, including homing and colonization, in the seminiferous tubules.…”
Section: Discussionmentioning
confidence: 99%
“…When the size reaches several millimeters in length along the tubule, the differentiation of germ cells commences at the centre of each colony 16 . These phenomena observed in transplantation experiments could be viewed as the homing of SSCs into their niches, followed by their proliferation and differentiation 17 .…”
Section: Colonization Of Injected Gs Cells In Cultured Tissuesmentioning
confidence: 99%
“…On the contrary, ITGA6 and ITGB1 were not immunologically detected in SG of testis cryosections [Schaller et al 1993]. In the mouse, ITGB1 and ITGA6 were identified as surface markers of SG [Shinohara et al 1999;Kanatsu-Shinohara et al 2008] which allowed the selection of cell fractions rich in SG stem cells by MACS and FACS [Shinohara et al 1999;Shinohara et al 2000;Kubota et al 2003;Phillips et al 2010].…”
Section: Introductionmentioning
confidence: 98%