Homobifunctional Imidoester Combined Black Phosphorus Nanosheets Used as Cofactors for Nucleic Acid Extraction

Liu, Huifang; Zou, Qingshuang; Kim, Myoung Gyu; Qiao, Zhen; Nguyen, Dao Thi Thuy; Koo, Bon San; Lee, Hyo Joo; Jang, Yoon Ok; Kim, Jun Ki; Shin, Yong Moon

doi:10.1007/s13206-022-00046-3

Cited by 8 publications

(9 citation statements)

References 31 publications

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“…The SdMDx system consists of a DMS-based AD PTFE filter and LAMP with LFA. Our previous studies have demonstrated that various biomaterial composites enable rapid, efficient, and affordable enrichment and separation of viral biomolecules for early clinical diagnosis [13] , [14] , [15] , [16] , [17] . Furthermore, our previous studies have shown that complex devices and expensive reagents are not needed for biomolecule separation steps, including pathogen enrichment and extraction [40] , [41] , [42] , [43] , [44] , [45] , [46] , and can be performed in POC settings.…”

Section: Discussionmentioning

confidence: 99%

“…Among the various materials, diatomaceous earth (DE) is a promising natural alternative to synthetic porous silica due to its biocompatibility, advantages in fabrication and transportability, and can be used for a broad range of environmental, biomedical, and physicochemical applications [11] , [12] . Moreover, our previous studies have demonstrated the potential of multifunctional DE composites in biological applications [13] , [14] , [15] , [16] , [17] . Thus, with the continuous development of biomaterial technologies, sample preparation processes can be prepared without precise equipment and expensive reagents.…”

Section: Introductionmentioning

confidence: 97%

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Self-directed molecular diagnostics (SdMDx) system for COVID-19 via one-pot processing

Jang

Kim²,

Roh

et al. 2023

Sensors and Actuators B: Chemical

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Self-directed molecular diagnostics (SdMDx) system for COVID-19 via one-pot processing

Jang

Kim²,

Roh

et al. 2023

Sensors and Actuators B: Chemical

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“…In the case of the existing extraction method for circulating NAs, a lysis step is performed to enhance the recovery rate of circulating NAs from a small sample volume. 7 , 29 , 64 , 65 , 66 Although a lysis step can extract a high concentration of circulating NAs, contamination with chaotropic reagents of cell‐derived genomic NAs, and prostate‐related NAs extracted from PCa cells and urinary exosomes may reduce the sensitivity and specificity in analyzing pure circulating NAs. From this perspective, further analysis and exploration of pure circulating NAs in urine through the development of a circulating NA enrichment and isolation platform without lysis are essential.…”

Section: Discussionmentioning

confidence: 99%

A novel platform using homobifunctional hydrazide for enrichment and isolation of urinary circulating RNAs

Koo

Kim

Jang

et al. 2022

Bioengineering & Transla Med

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Changes in specific circulating RNA (circRNA) expressions can serve as diagnostic noninvasive biomarkers for prostate cancer (PCa). However, there are still unmet needs, such as unclear types and roles of circRNAs, PCa detection in benign prostatic hyperplasia (BPH) by unstandardized methods, and limitations of sample volume capacity and low circRNA concentrations. This study reports a simple and rapid circRNA enrichment and isolation technique named “HAZIS‐CirR” for the analysis of urinary circRNAs. The method utilizes homobifunctional hydrazides with amine‐modified zeolite and polyvinylidene fluoride (PVDF) syringe filtration for combining electrostatic and covalent coupling and size‐based filtration, and it offers instrument‐free isolation of circRNAs in 20 min without volume limitation, thermoregulation, and lysis. HAZIS‐CirR has high capture efficiency (82.03%–92.38%) and a 10‐fold more sensitive detection limit (20 fM) than before enrichment (200 fM). The clinical utility of HAZIS‐CirR is confirmed by analyzing circulating mRNAs and circulating miRNAs in 89 urine samples. Furthermore, three miRNA panels that differentiate PCa from BPH and control, PCa from control, and BPH from control, respectively, are established by comparing miRNA levels. HAZIS‐CirR will be used as an optimal and established method for the enrichment and isolation of circRNAs as diagnostic, prognostic, and predictive biomarkers in human cancers.

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“…To overcome this limitation, further study would be desired with a larger cohort of clinical samples, varying by cancer stages as well as other samples, such as tissue and liquid biopsy, for testing the versatility of the HSSP. Finally, this HSSP assay should be applied to sample preparation techniques for clinical diagnostic systems [ 29 , 30 , 31 ]. Overall, this method is expected to be able to detect not only KRAS mutations but also other mutations by selectively designing a different sequence.…”

Section: Discussionmentioning

confidence: 99%

Hot-Spot-Specific Probe (HSSP) for Rapid and Accurate Detection of KRAS Mutations in Colorectal Cancer

et al. 2022

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Detection of oncogene mutations has significance for early diagnosis, customized treatment, treatment progression, and drug resistance monitoring. Here, we introduce a rapid, sensitive, and specific mutation detection assay based on the hot-spot-specific probe (HSSP), with improved clinical utility compared to conventional technologies. We designed HSSP to recognize KRAS mutations in the DNA of colorectal cancer tissues (HSSP-G12D (GGT→GAT) and HSSP-G13D (GGC→GAC)) by integration with real-time PCR. During the PCR analysis, HSSP attaches to the target mutation sequence for interference with the amplification. Then, we determine the mutation detection efficiency by calculating the difference in the cycle threshold (Ct) values between HSSP-G12D and HSSP-G13D. The limit of detection to detect KRAS mutations (G12D and G13D) was 5–10% of the mutant allele in wild-type populations. This is superior to the conventional methods (≥30% mutant allele). In addition, this technology takes a short time (less than 1.5 h), and the cost of one sample is as low as USD 2. We verified clinical utility using 69 tissue samples from colorectal cancer patients. The clinical sensitivity and specificity of the HSSP assay were higher (84% for G12D and 92% for G13D) compared to the direct sequencing assay (80%). Therefore, HSSP, in combination with real-time PCR, provides a rapid, highly sensitive, specific, and low-cost assay for detecting cancer-related mutations. Compared to the gold standard methods such as NGS, this technique shows the possibility of the field application of rapid mutation detection and may be useful in a variety of applications, such as customized treatment and cancer monitoring.

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Homobifunctional Imidoester Combined Black Phosphorus Nanosheets Used as Cofactors for Nucleic Acid Extraction

Cited by 8 publications

References 31 publications

Self-directed molecular diagnostics (SdMDx) system for COVID-19 via one-pot processing

Self-directed molecular diagnostics (SdMDx) system for COVID-19 via one-pot processing

A novel platform using homobifunctional hydrazide for enrichment and isolation of urinary circulating RNAs

Hot-Spot-Specific Probe (HSSP) for Rapid and Accurate Detection of KRAS Mutations in Colorectal Cancer

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