Homocysteine Levels and Clinical Outcomes in Schizophrenia—A Pilot Randomized Controlled Trial

Vedam, Venkata; Ghanta, Mohan Krishna; Kantipudi, Suvarna Jyothi; David, Darling Chellathai; Vijayalakshmi, M; Nuthalapati, Poojith

doi:10.1177/0976500x221128649

Cited by 2 publications

(1 citation statement)

References 55 publications

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“…The absorbance at 570nm was measured with UV-Spectrophotometer using DMSO as the blank. Measurements were performed and the concentration required for a 50% inhibition (IC50) was determined graphically [16]. The % cell viability was calculated using the following formula:…”

Section: Cell Culture and Treatmentmentioning

confidence: 99%

Green tea extract and hydroxyl-chloroquine combination enhances apoptosis in A549 non-small cell lung cancer cells

Suresh

2023

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Polyphenols, including catechins from green tea extract, have long been known for their potential anti-tumour activities. However, the precise mechanisms underlying their actions remain unclear. This study aimed to investigate the effects of green tea extract on A549 cells, a type of non-small lung cancer cells. A549 cells treated with green tea extract (GTE) were examined using an inverted light microscope and a fluorescence microscope. Cell sensitivity was evaluated using the MTT assay, while cell death was assessed using the Tali image-based cytometer. Ultra structural changes were observed using a transmission electron microscope. The findings suggested that even at the highest dose tested (150 μM), GTE did not exhibit toxic effects on A549 cells. Likewise, treatment with GTE resulted in a minimal, dose-dependent increase in the population of apoptotic cells. However, the analysis of cell structures using light and electron microscopy revealed an enhanced accumulation of vacuole-like structures in response to GTE. Moreover, under the fluorescence microscope, an increase in acidic vesicular organelles and the formation of LC3-II puncta were observed following GTE treatment. Assessment of autophagy function indicated that GTE-induced autophagy may serve as a self-protective mechanism against cytotoxicity, as blocking autophagy with bafilomycin A1 reduced cell viability and enhanced necrotic cell death in response to GTE treatment. In summary, our results demonstrate that A549 cells are insensitive to both low and high concentrations of green tea extract, likely due to the induction of cytoprotective autophagy. These findings suggest that the potential utility of GTE in lung cancer therapy may lie in its synergistic combinations with drugs or small molecules that target autophagy, rather than as a standalone therapy.

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Section: Cell Culture and Treatmentmentioning

confidence: 99%

Green tea extract and hydroxyl-chloroquine combination enhances apoptosis in A549 non-small cell lung cancer cells

Suresh

2023

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A Study of Disability Severity, Barriers, and Facilitating Factors in Accessing Healthcare Among Differently Abled Adults

Rahul,

Karunakaran

et al. 2024

Cureus

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Background: Disability impacts many individuals, thus restricting access to necessary healthcare. Barriers that affect health equity among people with disabilities include physical inaccessibility, financial constraints, and stigma in society. This study aims to report on the level of disability and factors determining healthcare access among adult differently abled persons in Chennai, Tamil Nadu, focusing on the WHO Disability Assessment Schedule (WHODAS 2.0). Methods: In this cross-sectional study, 300 differently abled adults affiliated with the Tamil Nadu Udavikkaram Association participated. The severity of disability was assessed using WHODAS 2.0, while a structured questionnaire elicited details on socio-demographic characteristics, barriers to healthcare utilization, and facilitating factors. Data was analyzed using SPSS version 16 (IBM Corp., Armonk, New York, USA), with descriptive statistics and odds ratios calculated for key variables. Results: Most participants (N=286, 93%) had mild levels of disability with problems mainly in self-care and mobility. The main barriers reported were healthcare expenses, distance to facilities, and lack of family support. Key facilitators were government schemes and assistive devices. Socio-economic factors like income and education showed marked correlations with the severity of disability. Conclusion: Healthcare access varies with disability severity, revealing a need for targeted financial, familial, and accessibility interventions to reduce disparity. Future longitudinal studies may provide insights into the development of policies and programs to support individuals living with disabilities.

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Homocysteine Levels and Clinical Outcomes in Schizophrenia—A Pilot Randomized Controlled Trial

Cited by 2 publications

References 55 publications

Green tea extract and hydroxyl-chloroquine combination enhances apoptosis in A549 non-small cell lung cancer cells

Green tea extract and hydroxyl-chloroquine combination enhances apoptosis in A549 non-small cell lung cancer cells

A Study of Disability Severity, Barriers, and Facilitating Factors in Accessing Healthcare Among Differently Abled Adults

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