Homocysteine: validation and comparison of two methods using samples from patients with pulmonary hypertension

Barbosa, Tatiana Maria Costa de Campos; Carvalho, Maria das Graças; Silveira, Josianne Nicácio; Rios, Júnia Garib; Komatsuzaki, Flávia; Godói, Lara Carvalho; Costa, Guilherme Hideki Yoshizane

doi:10.5935/1676-2444.20140048

Cited by 3 publications

(2 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The HPLC-FL method with SBD-F showed a good correlation with the results obtained using a bodipy-based fluorescence sensor for the determination of Hcy, Cys, and GSH in human serum [53]. In a clinical study, Hcy levels in patients with pulmonary hypertension [57], type 2 diabetes [58], and ulcerative colitis [59] were determined by HPLC-FL with SBD-F. A validated HPLC method for the routine determination of Hcy, Cys, and CA was developed [25] and applied to several hundred plasma samples. The results were used to examine the utility of carotid intima-media thickness [9] and cardio-ankle vascular index [8] as screening tools for atherosclerosis in the Japanese population.…”

Section: Fluorescence Detectionmentioning

confidence: 55%

HPLC Analysis of Homocysteine and Related Compounds

Wada¹,

Nakamura²,

Nakashima³

2018

Non-Proteinogenic Amino Acids

View full text Add to dashboard Cite

Homocysteine (Hcy), a sulfur-containing amino acid, is a representative intermediate metabolite of methionine (Met) to cysteine (Cys) via several intermediates. An elevated level of Hcy in plasma plays an important role in diseases such as neural tube defects and Down syndrome. Homocystinuria is the most common inborn error of sulfur metabolism and is caused by mutations in the metabolic enzymes of Hcy. These errors can be caused by abnormal levels of Met metabolites and classified on the basis of plasma Met levels. Additionally, Hcy and related compounds such as glutathione play an important role in maintaining homeostasis. Therefore, the simultaneous determination of Hcy and/or related compounds is required for appropriate clinical management of several diseases. The sulfur-containing amino acids and their derivatives in biological samples are quantified sensitively using high-performance liquid chromatography methods coupled with various detection methods such as UV/Vis, fluorescence, chemiluminescence, electrochemical, mass spectrometry, and tandem mass spectrometry. In this chapter, we review recent advances in these analytical methods and their applications.

show abstract

Section: Fluorescence Detectionmentioning

confidence: 55%

HPLC Analysis of Homocysteine and Related Compounds

Wada¹,

Nakamura²,

Nakashima³

2018

Non-Proteinogenic Amino Acids

View full text Add to dashboard Cite

show abstract

“…Oleh sebab itu, perlu dilakukan deteksi CAP secara akurat pada produk hewan untuk meminimalisir masalah diatas, salah satunya menggunakan instrumen High Performance Liquid Chromatography (HPLC). HPLC dipilih karena memiliki tingkat keakurasian dan sensitivitas yang tinggi, sehingga dapat digunakan untuk mendeteksi CAP secara akurat [6,7]. Namun, karena keberadaan CAP pada produk hewan pada umumnya dijumpai dalam konsentrasi yang rendah, maka sebelum melakukan instrumentasi perlu dilakukan pretreatment, salah satunya dengan cara pemekatan atau pre-concentration menggunakan Molecularly Imprinted Polymer (MIP) [8].…”

Section: Gambar 8 Struktur Kloramfenikol [2]unclassified

Pengaruh Variasi Komposisi Ekstraktan Terhadap Kemampuan Adsorpsi Kloramfenikol Menggunakan Molecularly Imprinted Polymer (Mip)

Listiadi¹,

Sianita²

2021

UJC

View full text Add to dashboard Cite

Penelitian ini bertujuan untuk mengoptimasi komposisi ekstraktan metanol:asam asetat dengan variasi 90:10(v/v), 85:15(v/v), dan 80:20(v/v) pada sintesis Molecularly Imprinted Polymer- Kloramfenikol (MIP-CAP). MIP-CAP diperoleh dari (Non Imprinted Polymer) NIP yang telah melalui proses ekstraksi template. Ekstraksi template menggunakan metode maserasi dan dianalisis menggunakan High Performance Liquid Chromatography (HPLC) untuk menentukan CAP terekstrak. Variasi komposisi ekstraktan terbaik ditentukan berdasarkan % ekstraksi dan kemampuan adsorpsi. Karakterisasi polimer MIP-CAP dilakukan dengan menggunakan Fourier Transform Infra Red (FTIR) untuk melihat keberadaan gugus –NO2. Pada penelitian dihasilkan komposisi ekstraktan metanol:asam asetat terbaik adalah 80:20 (v/v) (20% asam asetat) dengan perolehan % ekstraksi sebesar 89,5158% dan kemampuan adsorpsi sebesar 4,0725 mg/g dengan nilai Imprinting Factor (IF) sebesar 3,7369. Semakin tinggi penambahan asam asetat menyebabkan semakin tingginya % ekstraksi dan nilai kemampuan adsorpsi. Karaterisasi FTIR pada MIP-CAP menunjukkan tidak adanya serapan gugus -NO2. Kata kunci: CAP, MIP-CAP, Komposisi Ekstraktan

show abstract