Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis

Matsuda, Motoo; Tazumi, Akihiro; Kagawa, Shizuko; Sekizuka, Tsuyoshi; Murayama, Ohoshi; Je, Moore; Millar, B. Cherie

doi:10.1186/1746-6148-2-1

Cited by 27 publications

(19 citation statements)

References 19 publications

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“…Classification and percentage distribution of antibiotic resistance in commensal E. coli isolates against 10 different antibiotics is presented in Table 3 . All E. coli isolates were resistant to at least one antibiotic; these results are consistent with other previous studies on commensal E. coli , where commensal E. coli exhibited high prevalence of resistance to commonly used antibiotics in livestock [ 20 , 21 ] (Miles et al ., 2016). Furthermore, about 75.5% of bacterial isolate were resistant to more than two antibiotics (multidrug-resistant).…”

Section: Resultssupporting

confidence: 92%

Antibiotics Resistance Genes Screening and Comparative Genomics Analysis of Commensal Escherichia coli Isolated from Poultry Farms between China and Sudan

Abdelgader

Shi

Chen

et al. 2018

BioMed Research International

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Escherichia coli (E. coli) strains, from the gut of animals and humans, harbor wide range of drug resistance genes. A comparative study is conducted on the intestinal E. coli from fecal samples of healthy chicken from China and Sudan in order to monitor the antimicrobial sensitivity pattern. A number of 250 E. coli isolates from chicken farms, including 120 from China and 130 from Sudan, were isolated and identified. All isolates were subjected to susceptibility tests against 10 antibiotics and the distribution of antibiotic resistant genes was confirmed by PCR amplification, involving genes such as ampC, tetA, pKD13, acrA, ermA, ermB, ermC, tetB, mphA, aadA14, aadA1, aac3-1, and aac3- III. Many isolates were found to exhibit resistance against more than one antibiotic. However, the Chinese isolates showed more antibiotics resistance and resistance genes compared to the Sudanese isolates. For better understanding of the multidrug resistance factors, we conducted whole genome analyses of E. coli D107 isolated from China, which revealed that the genome possesses multiple resistance genes including tetracycline, erythromycin, and kanamycin. Furthermore, E. coli D4 isolate from Sudan was more sensitive to antibiotics such as erythromycin, tetracycline, and gentamicin. After analysis by RAST and MAUVE, the two strains showed 89% average nucleotide identity. However, the genomes mostly differed at the number of antibiotics-related genes, as the genome of D107 revealed a considerable number of antibiotics resistance genes such as ermA and mphD which were found to be absent in D4 genome. These outcomes provided confirmation that the poultry farms environment in different countries (China and Sudan) may serve as a potential reservoir of antimicrobial resistance genes and also indicated the evolutionary differences of strains in terms of resistant genes expression.

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Section: Resultssupporting

confidence: 92%

Antibiotics Resistance Genes Screening and Comparative Genomics Analysis of Commensal Escherichia coli Isolated from Poultry Farms between China and Sudan

Abdelgader

Shi

Chen

et al. 2018

BioMed Research International

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“…For instance, Bacillus anthracis shows VNTRs with repeat size ranging from 2 to 36 bp and array size from 1 to 23 repeats 36 , Salmonella enterica subsp. enterica shows VNTRs with monomer size between 6 bp and 189 bp, with array size of 4–15 repeats 37 , and Streptococcus uberus shows VNTRs from 12 to 208 bp monomer length and 2–5 repeats per array 38 . The high similarity of repeat size between bacteria and the animal and plant species analyzed here, and the resemblance of the short arrays length and dissemination pattern, suggest that satellite DNA is a common phenomenon to prokaryotes and eukaryotes.…”

Section: Discussionmentioning

confidence: 99%

High-throughput analysis of the satellitome illuminates satellite DNA evolution

Ruiz‐Ruano

López-León

Cabrero

et al. 2016

Sci Rep

207

307

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Satellite DNA (satDNA) is a major component yet the great unknown of eukaryote genomes and clearly underrepresented in genome sequencing projects. Here we show the high-throughput analysis of satellite DNA content in the migratory locust by means of the bioinformatic analysis of Illumina reads with the RepeatExplorer and RepeatMasker programs. This unveiled 62 satDNA families and we propose the term “satellitome” for the whole collection of different satDNA families in a genome. The finding that satDNAs were present in many contigs of the migratory locust draft genome indicates that they show many genomic locations invisible by fluorescent in situ hybridization (FISH). The cytological pattern of five satellites showing common descent (belonging to the SF3 superfamily) suggests that non-clustered satDNAs can become into clustered through local amplification at any of the many genomic loci resulting from previous dissemination of short satDNA arrays. The fact that all kinds of satDNA (micro- mini- and satellites) can show the non-clustered and clustered states suggests that all these elements are mostly similar, except for repeat length. Finally, the presence of VNTRs in bacteria, showing similar properties to non-clustered satDNAs in eukaryotes, suggests that this kind of tandem repeats show common properties in all living beings.

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“…Antibiotics are the drugs used for preventing economic losses caused by E. coli and increasing the production efficiency [ 10 ]. But increasing consumption of these drugs leads to scattering them into manure and other poultry wastes and transferring them to humans by their remains in carcasses and can be the origin of bacterial resistances, mortality, and increase of the human hospitalization in hospitals [ 3 , 15 ].…”

Section: Discussionmentioning

confidence: 99%

Multiple Antimicrobial Resistance ofEscherichia coliIsolated from Chickens in Iran

Talebiyan

Kheradmand

Khamesipour

et al. 2014

Veterinary Medicine International

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Antimicrobial agents are used extremely in order to reduce the great losses caused by Escherichia coli infections in poultry industry. In this study, 318 pathogenic Escherichia coli (APEC) strains isolated from commercial broiler flocks with coli-septicemia were examined for antimicrobials of both veterinary and human significance by disc diffusion method. Multiple resistances to antimicrobial agents were observed in all the isolates. Resistance to the antibiotics was as follows: Tylosin (88.68%), Erythromycin (71.70%), Oxytetracycline (43.40%), Sulfadimethoxine-Trimethoprim (39.62%), Enrofloxacin (37.74%), Florfenicol (35.85%), Chlortetracycline (33.96%), Doxycycline (16.98%), Difloxacin (32.08%), Danofloxacin (28.30%), Chloramphenicol (20.75%), Ciprofloxacin (7.55%), and Gentamicin (5.66%). This study showed resistance against the antimicrobial agents that are commonly applied in poultry, although resistance against the antibiotics that are only applied in humans or less frequently used in poultry was significantly low. This study emphasizes on the occurrence of multiple drug resistant E. coli among diseased broiler chickens in Iran. The data revealed the relative risks of using antimicrobials in poultry industry. It also concluded that use of antibiotics must be limited in poultry farms in order to reduce the antibiotic resistances.

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Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis

Cited by 27 publications

References 19 publications

Antibiotics Resistance Genes Screening and Comparative Genomics Analysis of Commensal Escherichia coli Isolated from Poultry Farms between China and Sudan

Antibiotics Resistance Genes Screening and Comparative Genomics Analysis of Commensal Escherichia coli Isolated from Poultry Farms between China and Sudan

High-throughput analysis of the satellitome illuminates satellite DNA evolution

Multiple Antimicrobial Resistance ofEscherichia coliIsolated from Chickens in Iran

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