Homogeneous electrochemical detection of ochratoxin A in foodstuff using aptamer–graphene oxide nanosheets and DNase I-based target recycling reaction

Sun, Ailing; Zhang, Yanfang; Sun, Guoqiang; Wang, Xuannian; Tang, Dianping

doi:10.1016/j.bios.2015.12.032

Cited by 125 publications

(28 citation statements)

References 48 publications

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“…Therefore, these electrodes are cumbersome to use for electrochemical aptasensing applications. The screen printed carbon electrode (SPCE) based biosensor offers the advantages over the conventional electrochemical biosensors in terms of disposability, portability and feasibility for on-site analysis at remote areas [14][15][16]. The preparation of SPCEs is simple, inexpensive and versatile with advantages of mass production and miniaturization of devices.…”

Section: Introductionmentioning

confidence: 99%

Disposable and portable electrochemical aptasensor for label free detection of aflatoxin B1 in alcoholic beverages

Goud

Catanante

Hayat

et al. 2016

Sensors and Actuators B: Chemical

122

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Section: Introductionmentioning

confidence: 99%

Disposable and portable electrochemical aptasensor for label free detection of aflatoxin B1 in alcoholic beverages

Goud

Catanante

Hayat

et al. 2016

Sensors and Actuators B: Chemical

122

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“…A number of different techniques have been developed in recent years for the detection of OTA, most of which are based on gas chromatography/mass spectrometry (GC/MS) and liquid chromatography (HPLC) . However, although these methods can correctly determine the presence of OTA, they require expensive equipment and complicated operator training . In this scenario, the design of simple, yet selective and sensitive, probes for the detection of this carcinogenic agent is a field of interest.…”

Section: Introductionmentioning

confidence: 99%

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

et al. 2017

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Aptamers have been used as recognition elements for several molecules due to their great affinity and selectivity. Additionally, mesoporous nanomaterials have demonstrated great potential in sensing applications. Based on these concepts, we report herein the use of two aptamer‐capped mesoporous silica materials for the selective detection of ochratoxin A (OTA). A specific aptamer for OTA was used to block the pores of rhodamine B‐loaded mesoporous silica nanoparticles. Two solids were prepared in which the aptamer capped the porous scaffolds by using a covalent or electrostatic approach. Whereas the prepared materials remained capped in water, dye delivery was selectively observed in the presence of OTA. The protocol showed excellent analytical performance in terms of sensitivity (limit of detection: 0.5–0.05 nm), reproducibility, and selectivity. Moreover, the aptasensors were tested for OTA detection in commercial foodstuff matrices, which demonstrated their potential applicability in real samples.

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“…Scheme of GO nanosheet-based homogeneous aptasensing system for detection of OTA on negatively charged SPCEs by coupling with DNaseI-based target recycling reaction and thionine-labeled OTA aptamer: target-induced dissociation of thionine-aptamer from GO nanosheet (A) and DNaseI-triggered target recycling detection (B). Reprinted from [66] with permission.…”

Section: Introductionmentioning

confidence: 99%

“…These molecules were captured by negatively charged SPCE producing an electrochemical signal at the applied potential. The electrochemical responses of THI at the SPCE were used as the analytical signals to be related with OTA concentrations from values as low as 5.6 pg·mL −1 [66]. Bacterial toxins, toxic substances produced and released by bacteria to target other bacterial or host cells, have also been determined using electrochemical affinity biosensors [67].…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical Affinity Biosensors in Food Safety

2017

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Safety and quality are key issues of today's food industry. Since the food chain is becoming more and more complex, powerful analytical methods are required to verify the performance of food safety and quality systems. Indeed, such methods require high sensitivity, selectivity, ability for rapid implementation and capability of automatic screening. Electroanalytical chemistry has, for decades, played a relevant role in food safety and quality assessment, taking more and more significance over time in the solution of analytical problems. At present, the implementation of electrochemical methods in the food is evident. This is in a large part due to the relevant results obtained by combining the attractive advantages of electrochemical transduction strategies (in terms of relatively simple hardware, versatility, interface with automatic logging and feasibility of application outside the laboratory environment) with those from biosensors technology. Important examples of enzyme electrochemical biosensors are those dedicated to the determination of glucose, alcohol or cholesterol are important examples. In addition, other types of different electrochemical biosensing approaches have emerged strongly in the last years. Among these, the strategies involving affinity interactions have been shown to possess a large number of applications. Therefore, electrochemical immunosensors and DNA-based biosensors have been widely used to determine major and minor components in foodstuffs, providing sufficient data to evaluate food freshness, the quality of raw materials, or the origin of samples, as well as to determine a variety of compounds at trace levels related to food safety such as micotoxins, allergens, drugs residues or pathogen microorganisms. This review discusses some critical examples of the latest advances in this area, pointing out relevant methodologies related to the measurement techniques, including the use of nanostructured electrodes and strategies for signal amplification.

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Homogeneous electrochemical detection of ochratoxin A in foodstuff using aptamer–graphene oxide nanosheets and DNase I-based target recycling reaction

Cited by 125 publications

References 48 publications

Disposable and portable electrochemical aptasensor for label free detection of aflatoxin B1 in alcoholic beverages

Disposable and portable electrochemical aptasensor for label free detection of aflatoxin B1 in alcoholic beverages

Two New Fluorogenic Aptasensors Based on Capped Mesoporous Silica Nanoparticles to Detect Ochratoxin A

Electrochemical Affinity Biosensors in Food Safety

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