Homogeneous stopped-flow fluorimmunoassay for gliadin determination in food samples

Sánchez-Martínez, M.L.; Aguilar-Caballos, M.P.; Gómez‐Hens, A.

doi:10.1016/j.aca.2004.07.011

Cited by 8 publications

(2 citation statements)

References 26 publications

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“…Because the anti-gliadin antibodies from the yolk that was used in the assay developed herein were polyclonal, the specificity of this assay must be evaluated. Most related works have tried to identify gliadin-free food by quantifying the gliadin in a test sample. , Therefore, in this work, eight cereal samples were processed to remove the water-soluble albumins and salt-soluble globulins before extraction with 60% ethanol to obtain gliadin in samples. − Figure summarizes the results of the QCM assays of cereals that underwent extraction using 60% ethanol. Besides wheat, only barley showed a significant frequency change among the other seven cereals (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles

Chu

Lin

Chen

et al. 2012

J. Agric. Food Chem.

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This work develops a label-free gliadin immunosensor that is based on changes in the frequency of a quartz crystal microbalance (QCM) chip. A higher sensitivity was obtained by applying 25 nm gold nanoparticles (AuNPs) to the surface of a bare QCM electrode. Subsequently, chicken anti-gliadin antibodies (IgY) were immobilized directly on the AuNP-modified surface by cross-linking amine groups in IgY with glutaraldehyde. Experimental results revealed that the change in frequency exhibited when 2 ppm gliadin was bound to the AuNP-modified electrode was 35 Hz (48%) greater than that of the bare gold electrode. The linear dynamic range in 60% ethanol was from 1 × 10(1) to 2 × 10(5) ppb gliadin, and the calculated limit of detection (LOD) was 8 ppb. The entire detection process was completed in 40 min and was highly repeatable. Additionally, the AuNP-modified QCM system generated results in the detection of gliadin in 10 commercial food products that were consistent with those obtained using an AOAC-approved gliadin kit. In conclusion, the QCM platform provides a potential alternative means of ensuring that people with wheat allergies and celiac patients have access to gliadin-free food.

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Section: Resultsmentioning

confidence: 99%

Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles

Chu

Lin

Chen

et al. 2012

J. Agric. Food Chem.

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“…Although gliadin is soluble in alcohol‐water mixtures ranging from 40 to 70 %, at this high concentrations a complete dissolution was never achieved. Therefore, a stock solution in DMSO 13 was prepared by adding 20 mg of gliadin to 10 mL of DMSO and subsequent vigorous sonication in a ultrasonic bath.…”

Section: Methodsmentioning

confidence: 99%

Amperometric Quantification of Gluten in Food Samples Using an ELISA Competitive Assay and Flow Injection Analysis

Amaya-González

de‐los‐Santos‐Álvarez

Lobo-Castañón

et al. 2010

Electroanalysis

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Gluten analysis in food is intricate because of the analyte variability among cereal species and protein states. Herein the immunodominant peptide 33-mer from alpha2-gliadin is proposed as standard for calibration in a competitive ELISA assay with amperometric flow-injection detection. Using the monoclonal antibody G12, a correlation between the content of gluten in peptide and gluten units was obtained for the first time. The analytical features were compared with optical measurements using several standards obtaining a slightly lower detection limit, which suggested the sensitivity is limited by affinity and not by the detection technique. "Gluten-free" samples were satisfactorily analyzed.

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A novel electrochemical labeled immunosensor for the detection of gliadin based on graphene oxide and silver nanoparticles

Şenol,

Koyuncu Zeybek

2024

Electroanalysis

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Homogeneous stopped-flow fluorimmunoassay for gliadin determination in food samples

Cited by 8 publications

References 26 publications

Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles

Detection of Gliadin in Foods Using a Quartz Crystal Microbalance Biosensor That Incorporates Gold Nanoparticles

Amperometric Quantification of Gluten in Food Samples Using an ELISA Competitive Assay and Flow Injection Analysis

A novel electrochemical labeled immunosensor for the detection of gliadin based on graphene oxide and silver nanoparticles

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