2016
DOI: 10.1016/j.jprot.2015.12.029
|View full text |Cite
|
Sign up to set email alerts
|

Homogenization of tissues via picosecond-infrared laser (PIRL) ablation: Giving a closer view on the in-vivo composition of protein species as compared to mechanical homogenization

Abstract: Posttranslational modifications and proteolytic processing regulate almost all physiological processes. Dysregulation can potentially result in pathologic protein species causing diseases. Thus, tissue species proteomes of diseased individuals provide diagnostic information. Since the composition of tissue proteomes can rapidly change during tissue homogenization by the action of enzymes released from their compartments, disease specific protein species patterns can vanish. Recently, we described a novel, ultr… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1

Citation Types

2
42
0

Year Published

2017
2017
2021
2021

Publication Types

Select...
6
1
1

Relationship

0
8

Authors

Journals

citations
Cited by 35 publications
(44 citation statements)
references
References 36 publications
2
42
0
Order By: Relevance
“…The method has been particularly applicable as a surgical technique with minimized collateral damage to surrounding tissue (Amini-Nik et al, 2010;Petersen et al, 2016). Recently, we have demonstrated PIRL-DIVE ablation for the soft extraction of structurally and even functionally intact biomolecular complexes such as large protein complexes ($520 kDa) and viruses ($40 MDa) as well as unmodified protein species, directly from aqueous solution and tissue (Kwiatkowski et al, 2015(Kwiatkowski et al, , 2016Ren et al, 2015).…”
Section: Introductionmentioning
confidence: 99%
“…The method has been particularly applicable as a surgical technique with minimized collateral damage to surrounding tissue (Amini-Nik et al, 2010;Petersen et al, 2016). Recently, we have demonstrated PIRL-DIVE ablation for the soft extraction of structurally and even functionally intact biomolecular complexes such as large protein complexes ($520 kDa) and viruses ($40 MDa) as well as unmodified protein species, directly from aqueous solution and tissue (Kwiatkowski et al, 2015(Kwiatkowski et al, , 2016Ren et al, 2015).…”
Section: Introductionmentioning
confidence: 99%
“…The ablation process was shown to be soft enough to extract proteins from tissue without any change in their chemical composition, and samples of human blood plasma were shown to have detectable enzymatic activities after sample treatment. PIRL‐DIVE showed a high quality of tissue homogenate with a higher number of intact proteins and recovery compared to mechanical homogenization (Kwiatkowski et al, ).…”
Section: Mechanical and Physical Procedures To Facilitate Protein Retmentioning
confidence: 99%
“…At the same time, efficient energy redistribution into thermal modes is ensured by keeping the irradiance low enough to avoid nonlinear optical excitation of higher-lying molecular states. Picosecond infrared laser (PIRL) irradiation fulfilling these criteria has been shown to be well-suited for the extraction of large bio-molecules and even intact viruses for subsequent offline analysis, [20][21][22] as an atmospheric online ion source in combination with electrospray ionization (LAESI), 23 and has been suggested as a potential tool for sample-delivery in high-speed X-ray crystallography. 24 Moreover, the absence of ionizing radiation and minimal deposition of thermal and acoustic energy into the non-ablated sample surface make DIVE a valuable tool for precise material removal in surgical applications.…”
Section: Introductionmentioning
confidence: 99%