Homoisoflavanone prevents mast cell activation and allergic responses by inhibition of Syk signaling pathway

Abstract

Homoisoflavanone inhibited mast cell activation through the suppression of Syk pathway together with the inhibition of cPLA2 . Thus, it might be a good candidate molecule for allergic diseases.

Cinnamaldehyde derivatives inhibit degranulation and inflammatory mediator production in rat basophilic leukemia cells

“…During degranulation, the secretory granules of mast cells can fuse with plasma membrane using SNAREs, and prestored molecules, such as histamine and β-hexosaminidase, are then released into the extracellular environment [8,43,44]. Thus, the release of β-hexosaminidase is widely used for an indicator of degranulation of mast cells [12,[14][15][16][17]19,23,45]. In this context, the effect of cinnamaldehyde derivatives on degranulation was assessed by β-hexosaminidase assay.…”

“…The effects of IgE and PMA/A23187 on mast cells are known to activate PKC and extracellular Ca 2 + uptake [6,11,23,42,44,46]. These effects control degranulation, cytoskeleton rearrangement, and gene expression of pro-inflammatory cytokines in mast cells [5-8, 19,46].…”

“…Pro-inflammatory mediators such as TNF-α, IL-4, and COX-2 are crucial for allergic and inflammatory responses [4,[16][17][18][19][20][21][22][23]. We measured the effects of 4-chloro-cinnamaldehyde and 4-trifluorocinnamaldehyde on the relative gene expression of pro-inflammatory mediators in RBL-2H3 cells (Fig.…”

“…Additionally, the activation of PKC and increased Ca 2+ influx, stimulated by PMA and A23187, respectively, are critical factors for allergic responses of mast cells [7,19,21,22]. Thus, PMA and A23187 stimulate mast cells to release inflammatory mediators related to allergic responses [12,13,23].…”

Capsiate Inhibits DNFB-Induced Atopic Dermatitis in NC/Nga Mice through Mast Cell and CD4+ T-Cell Inactivation

“…BMMCs were generated as previously described, with minor modifications (Frossi et al, 2007;Lee et al, 2014). Briefly, bone marrow cells were obtained from tibias and femurs of 8-12-week-old C57BL/6 mice.…”