Homologous Acellular Matrix Graft for Urethral Reconstruction in the Rabbit: Histological and Functional Evaluation

Sievert, Karl‐Dietrich; Bakırcıoğlu, Emre; Nunes, Lora; Tu, Richard; Dahiya, Rajvir; Tanagho, Emil A.

doi:10.1016/s0022-5347(05)67610-0

Cited by 103 publications

(45 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recent advances in the field of tissue engineering have prompted researchers to reconstitute tissue using cultured cells and extracellular matrix [Sievert et al, 2000;Knight et al, 2005]. Amano [2003] reported that porcine corneal stroma, expressing little ␣ -gal epitope, induced no superacute rejection but only mild cellular rejection when transplanted to the corneas of cynomolgus monkeys.…”

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Shao

et al. 2010

Cells Tissues Organs

View full text Add to dashboard Cite

Background: The global shortage of donor corneas has motivated the development of bioengineered corneas. Although corneal endothelium has been reconstituted using corneal endothelial cells (CEC) and precursor cells with various carrier materials, all of the current options require corneal tissue and are also limited by the scarcity of donor corneas. Here, we explored the feasibility of inducing bone marrow-derived endothelial progenitor cells (BEPC) to differentiate into CEC for the repair of corneal endothelial defects. Materials and Methods: BEPC were isolated from human fetal bone marrow, and identified using several antigen markers. BEPC were cocultured with CEC for 10 days in a transwell system with conditioned medium from CEC, and cell transdifferentiation was then examined. With a porcine corneal acellular matrix (PCACM) as the carrier, the induced BEPC were transplanted onto a cat’s cornea from which Descemet’s membrane and the endothelium had been stripped. Results: The induced BEPC resembled CEC in polygonal shape, expressing aquaporin-1, tightly opposed cell junctions, and neurone-specific enolase. Twenty-eight days after surgery, the transparency gradually returned to the corneas transplanted with the induced BEPC on PCACM. Conclusions: Human fetal BEPC transdifferentiate into corneal endothelial-like cells in vitro. Features of the induced BEPC indicated that they may be useful for the repair of corneal endothelial dysfunction.

show abstract

Section: Discussionmentioning

confidence: 99%

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Shao

et al. 2010

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…Urinary bladder-derived ECM has been used in numerous regenerative medicine applications, including bladder regeneration, urethral repair, and esophageal repair. 12,27,28 Liver-derived ECM has also been evaluated as a biological scaffold for use in regenerative medicine. 29 The antimicrobial peptides present in the degradation products of UBM-ECM and L-ECM were released using acid/heat digestion and fractionated using ammonium sulfate precipitation.…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Brennan

Reing²,

Chew³

et al. 2006

Tissue Engineering

210

133

View full text Add to dashboard Cite

Biological scaffolds composed of extracellular matrix (ECM) have been shown to be resistant to deliberate bacterial contamination in preclinical in vivo studies. The present study evaluated the degradation products resulting from the acid digestion of ECM scaffolds for antibacterial effects against clinical strains of Staphylococcus aureus and Escherichia coli. The ECM scaffolds were derived from porcine urinary bladder (UBM-ECM) and liver (L-ECM). These biological scaffolds were digested with acid at high temperatures, fractionated using ammonium sulfate precipitation, and tested for antibacterial activity in a standardized in vitro assay. Degradation products from both UBM-ECM and L-ECM demonstrated antibacterial activity against both S. aureus and E. coli. Specific ammonium sulfate fractions that showed antimicrobial activity varied for the 2 different ECM scaffold types. The results of this study suggest that several different lowmolecular-weight peptides with antibacterial activity exist within ECM and that these peptides may help explain the resistance to bacterial infection provided by such biological scaffolds.

show abstract

“…Many tissue-engineered substitutes, for example, bioartificial skin (Gentzkow et al, 1996;Hansbrough et al, 1994), esophagus (Takimoto et al, 1998), and urethra (Sievert et al, 2000), have already been developed in vitro and some have been used clinically (Brittberg et al, 1994). The common features of these products are that they are quite thin tissues of less than several millimeters in thickness, and therefore very different from the primary organs, and that they are composed of single or a few types of cells with poorly organized blood capillaries.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of endothelialized tube in collagen gel as starting point for self‐developing capillary‐like network to construct three‐dimensional organs in vitro

Takei¹,

Sakai²,

Ono³

et al. 2006

Biotech & Bioengineering

View full text Add to dashboard Cite

A possible strategy for creating three-dimensional (3D) tissue-engineered organs in vitro with similar volumes to the primary organs is to develop a capillary network throughout the constructs to provide sufficient oxygenation and nutrition to the cells composing them. Here, we propose a novel approach for the creation of a capillary-like network in vitro, based on the spontaneous tube-forming activity of vascular endothelial cells (ECs) in collagen gel. We fabricated a linear tube of 500 microm in diameter, the inner surface of which was filled with bovine carotid artery vascular endothelial cells (BECs), in type I collagen gel as a starting point for the formation of a capillary-like network. The BECs exposed to a medium containing vascular endothelial growth factor (VEGF) migrated into the ambient gel around the tube. After 2 weeks of VEGF exposure, the distance of the migration into the ambient gel in the radial direction of the tube reached approximately 800 microm. Cross-sections of capillary-like structures composed of the migrating BECs, with a lumen-like interior space, were observed in slices of the gel around the tube stained with hematoxylin-eosin (H&E). These results demonstrate that this approach using a pre-established tube, which is composed of ECs, as a starting point for a self-developing capillary-like network is potentially useful for constructing 3D organs in vitro.

show abstract

Homologous Acellular Matrix Graft for Urethral Reconstruction in the Rabbit: Histological and Functional Evaluation

Cited by 103 publications

References 17 publications

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Bone Marrow-Derived Endothelial Progenitor Cells: A Promising Therapeutic Alternative for Corneal Endothelial Dysfunction

Antibacterial Activity within Degradation Products of Biological Scaffolds Composed of Extracellular Matrix

Fabrication of endothelialized tube in collagen gel as starting point for self‐developing capillary‐like network to construct three‐dimensional organs in vitro

Contact Info

Product

Resources

About