Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization

Mota, I.; Wong, Daniel

doi:10.1016/0024-3205(69)90099-x

Cited by 252 publications

(139 citation statements)

References 11 publications

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“…The specific IgE antibody titer was determined from the heterologous PCA reaction in the dorsal skin of male Sprague-Dawley rats. 13 ) One-tenth of a milliliter of the pooled serum serially diluted with PBS was injected intradermally into the shaved back of an SD rat. After 3 hr, the rat was challenged by intravenous injection of 1mg of antigen in 1ml of 0.5% Evans blue dye dissolved in PBS.…”

Section: Methodsmentioning

confidence: 99%

Reduction of Ovomucoid Immunogenic Activity on Peptic Fragmentation and Heat Denaturation

Matsuda

Tsuruta

Nakabe

et al. 1985

Agricultural and Biological Chemistry

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Section: Methodsmentioning

confidence: 99%

Reduction of Ovomucoid Immunogenic Activity on Peptic Fragmentation and Heat Denaturation

Matsuda

Tsuruta

Nakabe

et al. 1985

Agricultural and Biological Chemistry

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“…CPSMV-specific IgG1 antibodies were detected in pooled mouse sera using a 2-h latent period for skin sensitization (25). Intradermal injections of the antisera or their dilutions were made into the dorsal skin of shaved normal mice.…”

Section: Passive Cutaneous Anaphylaxis For Igg1 and Igementioning

confidence: 99%

Immune response induced in mice oral immunization with cowpea severe mosaic virus

Florindo

Aragão

Silva

et al. 2002

Braz J Med Biol Res

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There is increasing interest in the immune response induced by plant viruses since these could be used as antigen-expressing systems in vaccination procedures. Cowpea severe mosaic virus (CPSMV), as a purified preparation (300 g of leaves, 2 weeks post-inoculation), or crude extract from cowpea (Vigna unguiculata) leaves infected with CPSMV both administered by gavage to Swiss mice induced a humoral immune response. Groups of 10 Swiss mice (2-month-old females) were immunized orally with 10 daily doses of either 50 µg viral capsid protein (boosters of 50 µg at days 21 and 35 after immunization) or 0.6 mg protein of the crude extract (boosters of 0.6 mg at days 21 and 35 after immunization). Anti-CPSMV antibodies were quantified by ELISA in pooled sera diluted at least 1:400 at days 7, 14, 21, 28, 35 and 42 after the 10th dose. IgG and IgA against CPSMV were produced systemically, but IgE was not detected. No synthesis of specific antibodies against the proteins of leaf extracts from V. unguiculata, infected or not with CPSMV, was detected. The use of CPSMV, a plant-infecting virus that apparently does not induce a pathogenic response in animals, induced a humoral and persistent (at least 6 months) immune response through the administration of low antigen doses by gavage. These results raise the possibility of using CPSMV either as a vector for the production of vaccines against animal pathogens or in quick and easy methods to produce specific antisera for viral diagnosis.

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“…The allergenic properties of A. suum components were determined by their ability to induce IgE measured by passive cutaneous anaphylaxis (PCA) in rats according to the method of Mota and Wong (10). Several dilutions of the serum from mice immunized with Al(OH) 3 (7.5 mg) plus 250 µl of each fraction of A. suum crude extract eluted on a Sephacryl S-300 column were injected intradermally into the shaved back of three rats.…”

mentioning

confidence: 99%

Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component

Pires

Oshiro

Itami

et al. 2001

Braz J Med Biol Res

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Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization

Cited by 252 publications

References 11 publications

Reduction of Ovomucoid Immunogenic Activity on Peptic Fragmentation and Heat Denaturation

Reduction of Ovomucoid Immunogenic Activity on Peptic Fragmentation and Heat Denaturation

Immune response induced in mice oral immunization with cowpea severe mosaic virus

Production and characterization of a monoclonal antibody against an Ascaris suum allergenic component

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