Homologous Overexpression of Diacylglycerol Acyltransferase in Oleaginous Fungus <i>Mucor circinelloides</i> WJ11 Enhances Lipid Accumulation under Static Solid Cultivation

Xin, Feifei; Chang, Yufei; Gao, Meng; Xie, Zhike; Yang, Weiren; Chen, Mei‐Ling; Zhang, Huaiyuan; Song, Yuanda

doi:10.1021/acs.jafc.2c03489

Cited by 3 publications

(3 citation statements)

References 47 publications

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“…The promotion of DGAT on lipid accumulation has been reported in many previous studies. The homologous overexpression of McDGAT2d gave an improvement to lipid accumulation under static solid fermentation [ 30 ], and the overexpression of endogenous PtDGAT1 increased by more than double in TG and total lipid compared to that of the wild-type Phaeodactylum tricornutum , which reached 57% and 73% of dry weight, respectively [ 31 ]. In addition, the overexpression of endogenous PtDGAT2B resulted in enhancing the neutral lipid content by 69% [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

Chang

Cao

et al. 2023

JoF

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Triacylglycerol (TG) with high-value long-chain polyunsaturated fatty acids is beneficial to human health; consequently, there is an urgent need to broaden its sources due to the current growing demand. Mortierella alpina, one of the most representative oleaginous fungi, is the only certificated source of dietary arachidonic acid-rich oil supplied in infant formula. This study was conducted to improve TG production in M. alpina by homologous overexpression of diacylglycerol acyltransferase (DGAT) and linseed oil (LSO) supplementation. Our results showed that the homologous overexpression of MaDGAT1B and MaDGAT2A strengthened TG biosynthesis and significantly increased the TG content compared to the wild-type by 12.24% and 14.63%, respectively. The supplementation with an LSO concentration of 0.5 g/L elevated the TG content to 83.74% and total lipid yield to 4.26 ± 0.38 g/L in the M. alpina-MaDGAT2A overexpression strain. Our findings provide an effective strategy for enhancing TG production and highlight the role of DGAT in TG biosynthesis in M. alpina.

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Section: Discussionmentioning

confidence: 99%

Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

Chang

Cao

et al. 2023

JoF

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“…circinelloides strain WJ11 (wild-type) [6] was used as a reference in this study. M. circinelloides WJ11 defective in the carRP gene (∆carRP WJ11) was employed for investigating lipid and carotenoid biosynthesis, with reference to the Mc2075 strain with knock out of the carRP gene performed through homologous recombination by using the designed pMAT2075 plasmid [28].…”

Section: Fungal Strains and Cultivationsmentioning

confidence: 99%

“…However, the downregulation of acetyl-CoA synthetase (EC: 6.2.1.1) and ATP citrate lyase (EC: 2.3.3.8) might lead to a reduction in the acetyl-CoA pool for fatty acid synthesis [28]. Downregulation of fatty acid synthase (FAS1) (EC: 2.3.1.86), involved in long-chain fatty acid synthesis, was also found in ∆carRP WJ11.…”

Section: Transcriptome Data and Degs Across Pairwise Comparisons Of W...mentioning

confidence: 99%

Dissecting Holistic Metabolic Acclimatization of Mucor circinelloides WJ11 Defective in Carotenoid Biosynthesis

Li,

Thananusak,

Raethong

et al. 2024

Biology

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Mucor circinelloides WJ11 is a lipid-producing strain with industrial potential. A holistic approach using gene manipulation and bioprocessing development has improved lipid production and the strain’s economic viability. However, the systematic regulation of lipid accumulation and carotenoid biosynthesis in M. circinelloides remains unknown. To dissect the metabolic mechanism underlying lipid and carotenoid biosynthesis, transcriptome analysis and reporter metabolites identification were implemented between the wild-type (WJ11) and ΔcarRP WJ11 strains of M. circinelloides. As a result, transcriptome analysis revealed 10,287 expressed genes, with 657 differentially expressed genes (DEGs) primarily involved in amino acid, carbohydrate, and energy metabolism. Integration with a genome-scale metabolic model (GSMM) identified reporter metabolites in the ΔcarRP WJ11 strain, highlighting metabolic pathways crucial for amino acid, energy, and nitrogen metabolism. Notably, the downregulation of genes associated with carotenoid biosynthesis and acetyl-CoA generation suggests a coordinated relationship between the carotenoid and fatty acid biosynthesis pathways. Despite disruptions in the carotenoid pathway, lipid production remains stagnant due to reduced acetyl-CoA availability, emphasizing the intricate metabolic interplay. These findings provide insights into the coordinated relationship between carotenoid and fatty acid biosynthesis in M. circinelloides that are valuable in applied research to design optimized strains for producing desired bioproducts through emerging technology.

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Transcriptomic Analysis Revealed the Differences in Lipid Accumulation between Spores and Mycelia of Mucor circinelloides WJ11 under Solid–State Fermentation

et al. 2022

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The oleaginous fungus Mucor circinelloides has been studied for microbial oil production. Solid–state fermentation may be more suitable for lipid production than submerged fermentation due to its special filamentous structure and lower fermentation costs. M. circinelloides WJ11 under solid–state fermentation indicated that the total fatty acid content of mycelia was significantly higher than that of spores (15.0 and 10.4% in mycelia and spores after 192 h, respectively), while the biomass of the fungal mycelia was lower than that of the spores, reaching 78.2 and 86.9 mg/g, respectively. Transcriptomic studies showed that a total of 9069 genes were differentially expressed between spores and mycelia during solid–state fermentation, of which 4748 were up-regulated and 4321 were down-regulated. Among them, triglyceride-related synthases in M. circinelloides were significantly up-regulated in the mycelia. The mRNA expression level of ATP: citrate lyase was obviously increased to provide more acetyl-CoA for fatty acid synthesis in mycelia, moreover, the metabolism of leucine and isoleucine can also produce more acetyl-CoA for lipid accumulation in M. circinelloides. For NADPH supply, the expression of the pentose phosphate pathway was significantly up-regulated in mycelia, while NADP+-dependent malic enzyme was also increased by 9.5-fold under solid–state fermentation. Compared with gene expression in spores, the autophagy pathway was clearly up-regulated in mycelia to prove that autophagy was related to lipid accumulation in M. circinelloides.

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Homologous Overexpression of Diacylglycerol Acyltransferase in Oleaginous Fungus Mucor circinelloides WJ11 Enhances Lipid Accumulation under Static Solid Cultivation

Cited by 3 publications

References 47 publications

Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

Applications of Diacylglycerol Acyltransferase for Triacylglycerol Production in Mortierella alpina

Dissecting Holistic Metabolic Acclimatization of Mucor circinelloides WJ11 Defective in Carotenoid Biosynthesis

Transcriptomic Analysis Revealed the Differences in Lipid Accumulation between Spores and Mycelia of Mucor circinelloides WJ11 under Solid–State Fermentation

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