Homologous Region 3 fromBombyx moriNucleopolyhedrovirus Enhancing the Transcriptional Activity of Heat Shock Cognate 70-4 Promoter fromBombyx moriandBombyx mandarinain Vitroandin Vivo

Tang, Shengnan; Zhao, Qiaoling; Yao, Yi; Zhang, Zhifang; Li, Yiren

doi:10.1271/bbb.69.1014

Cited by 12 publications

(4 citation statements)

References 10 publications

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“…On the other hand, it has been supposed that HSPs act to chaperone viruses, are involved with the assembly and production of progeny virus, and thereby facilitate viral replication [34] . In addition, Tang et al [35] found that the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) can significantly increase the transcriptional activity of BmHSC70-4p and HSC70-4p from Bombyx mandarina (BmandHSC70-4p). Taken together, we hold that protein HSP70 is not the result of virus infection, but an accomplice to the infection.…”

Section: Identification Of Host-derived Proteinsmentioning

confidence: 99%

Determination of Protein Composition and Host-Derived Proteins of Bombyx mori Nucleopolyhedrovirus by 2-Dimensional Electrophoresis and Mass Spectrometry

et al. 2008

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Objective: The occlusion-derived form of baculovirus is specially adapted for primary infection of host midgut epithelial cells. The virion contains proteins essential for host-range determination and initiation of infection. Determination of protein composition of the occlusion-derived virus (ODV) will help us to understand this virion’s infection mechanism. Methods: We obtained the ODV virion of Bombyx mori nucleopolyhedrovirus (BmNPV) by sucrose density gradient centrifugation, and used SDS-PAGE, 2-dimensional electrophoresis and mass spectrometry to identify – for the first time – proteins present within or associated with the ODV virion. Results: 20 proteins, including 16 major proteins of the virion encoded by BmNPV and 4 proteins of the silkworm, were identified with confidence. Conclusion: Three BmNPV proteins (orf40, P95 and protein tyrosine phosphatase), which had not been detected as components of ODV previously, were identified in this study. We propose that the host-derived proteins may be important for the assembly and maturation of the ODV capsid.

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Section: Identification Of Host-derived Proteinsmentioning

confidence: 99%

Determination of Protein Composition and Host-Derived Proteins of Bombyx mori Nucleopolyhedrovirus by 2-Dimensional Electrophoresis and Mass Spectrometry

et al. 2008

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“…Previous studies have shown that B. mori baculovirus promoters are functional in expression vectors and display expected transcriptional activity when injected into silkworm larvae (Zhou et al 2002;Chen et al 2004;Tang et al 2005). To assess the efficiency of this method in the pupal wing, we generated a constitutive reporter encoding the luciferase gene under the control of the previously described ie-1 promoter and hr3 enhancer (pBacPAK8-ie-1-luc-hr3; Chen et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Homology of Dipteran Bristles and Lepidopteran Scales: Requirement for the Bombyx mori achaete-scute Homologue ASH2

Zhou

Shen

et al. 2009

Genetics

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Lepidopteran wing scales and Drosophila bristles are considered homologous structures on the basis of the similarities in their cell lineages. However, the molecular mechanisms underlying scale development are essentially unknown as analysis of gene function in Lepidoptera is sorely limited. In this study, we used the Bombyx mori mutant scaleless (sl), which displays a nearly complete loss of wing scales, to explore the mechanism of lepidopteran wing-scale formation. We found that Bm-ASH2, one of four Bombyx achaetescute homologs, is highly expressed in early pupal wings of wild-type silkworms, but its expression is severely reduced in sl pupal wings. Through molecular characterization of the mutant locus using luciferase and gel shift assays, genetic analysis of recombining populations, and in vivo rescue experiments, we provide evidence that a 26-bp deletion within the Bm-ASH2 promoter is closely linked to the sl locus and leads to loss of Bm-ASH2 expression and the scaleless-wings phenotype. Thus, the Bm-ASH2 appears to play a critical role in scale formation in B. mori. This finding supports the proposed homology of lepidopteran scales and dipteran bristles and provides evidence for conservation of the genetic pathway in scale/bristle development at the level of gene function.

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“…The cycling protocol for PCR was the following: 1 cycle at 94°C for 3 min; 5 cycles at 94°C for 30 s, 50°C for 30 s and 72°C for 30 s; then 30 cycles at 94°C for 30 s, 55°C for 30 s and 72°C for 30 s; and a final extension step of 10 min at 72°C. The PCR products were analysed on 1% agarose gels and purified using the glass-milk method 30 . The purified fragment was then cloned into the pMD18-T vector and sequenced.…”

Section: Molecular Cloning Of Rmhe Cdnamentioning

confidence: 99%

Molecular cloning and identification of the hatching enzyme gene in Rondotia menciana

Tang¹,

Wang²,

Wang³

et al. 2018

ScienceAsia

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Rondotia menciana Moore (Lepidoptera: Bombycidae) is a major pest of mulberry. We cloned the fulllength cDNA of the hatching enzyme from R. menciana eggs (RmHE) using the RACE-PCR and SMART cDNA synthesis technique. The cDNA is 1325 bp long and contains an ORF of 885 bp. It encodes 294 amino acid residues, including a putative signal peptide of 16 amino acid residues. Two conserved signature sequences of insect hatching enzyme (HE) harbour in the RmHE. The deduced amino acid sequence of RmHE has 30-84% identity to HE sequences in other insect species, and RmHE is most similar to that of wild silkworm HE. RmHE transcripts could be detected in R. menciana eggs at different developmental stages. Levels remained low during the early stages, increased greatly in 7-d eggs, and reached a maximum in 9-d eggs. Changes in the levels of RmHE transcripts occurred in accordance with the process of embryo development and eclosion. This indicated that RmHE plays an important role in these processes. RmHE transcripts were also detected in larval midguts, suggesting that RmHE is involved in food digestion. As far as we know, this is the first report on an HE gene in Lepidopteran pests and will offer some basic information for developing an efficient control method during the hatching process for agricultural Lepidopteran pests.

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Homologous Region 3 fromBombyx moriNucleopolyhedrovirus Enhancing the Transcriptional Activity of Heat Shock Cognate 70-4 Promoter fromBombyx moriandBombyx mandarinain Vitroandin Vivo

Cited by 12 publications

References 10 publications

Determination of Protein Composition and Host-Derived Proteins of <i>Bombyx mori</i> Nucleopolyhedrovirus by 2-Dimensional Electrophoresis and Mass Spectrometry

Determination of Protein Composition and Host-Derived Proteins of <i>Bombyx mori</i> Nucleopolyhedrovirus by 2-Dimensional Electrophoresis and Mass Spectrometry

Homology of Dipteran Bristles and Lepidopteran Scales: Requirement for the Bombyx mori achaete-scute Homologue ASH2

Molecular cloning and identification of the hatching enzyme gene in Rondotia menciana

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