Homologs of Late Blight Resistance Genes in Representatives of Tuber-Bearing Species of the Genus Solanum L.

Gurina, A. A.; Alpatieva, N. V.; Chalaya, N. A.; Мироненко, Н. В.; Хютти, А. В.; Рогозина, Е. В.

doi:10.1134/s1022795422120043

Cited by 2 publications

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“…While in S. bulbocastanum these genome fragments were linked to resistance to P. infestans , in other species such a relationship was not observed [ 33 , 93 , 94 ]. The structural homologs of Rpi-vnt1 initially identified in S. venturii were found in many South and North American species ([ 54 , 99 , 100 ] and this communication); most probably they are pseudogenes.…”

Section: General Discussion and Conclusionmentioning

confidence: 80%

“…Using allele mining with specific PCR primers, Pel [ 99 ] recognized the functional and non-functional homologs of this gene in many South American species. The list of species comprising these homologs was recently complemented with S. alandiae (OP889280) and S. okadae (OP903198) [ 62 ] and with S. phureia and S. stenotomum [ 100 ]. In the current study, the marker of the Rpi-vnt1 gene was also registered in two Tuberosa species absent from the Pel’s list: S. alandiae and S. berthaultii ( Table 2 ).…”

Section: General Discussion and Conclusionmentioning

confidence: 99%

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Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Рогозина

Gurina

Chalaya

et al. 2023

Plants

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Late blight (LB) caused by the oomycete Phytophthora infestans (Mont.) de Bary is the greatest threat to potato production worldwide. Current potato breeding for LB resistance heavily depends on the introduction of new genes for resistance to P. infestans (Rpi genes). Such genes have been discovered in highly diverse wild, primitive, and cultivated species of tuber-bearing potatoes (Solanum L. section Petota Dumort.) and introgressed into the elite potato cultivars by hybridization and transgenic complementation. Unfortunately, even the most resistant potato varieties have been overcome by LB due to the arrival of new pathogen strains and their rapid evolution. Therefore, novel sources for germplasm enhancement comprising the broad-spectrum Rpi genes are in high demand with breeders who aim to provide durable LB resistance. The Genbank of the N.I. Vavilov Institute of Plant Genetic Resources (VIR) in St. Petersburg harbors one of the world’s largest collections of potato and potato relatives. In this study, LB resistance was evaluated in a core selection representing 20 species of seven Petota series according to the Hawkes (1990) classification: Bulbocastana (Rydb.) Hawkes, Demissa Buk., Longipedicellata Buk., Maglia Bitt., Pinnatisecta (Rydb.) Hawkes, Tuberosa (Rydb.) Hawkes (wild and cultivated species), and Yungasensa Corr. LB resistance was assessed in 96 accessions representing 18 species in the laboratory test with detached leaves using a highly virulent and aggressive isolate of P. infestans. The Petota species notably differed in their LB resistance: S. bulbocastanum Dun., S. demissum Lindl., S. cardiophyllum Lindl., and S. berthaultii Hawkes stood out at a high frequency of resistant accessions (7–9 points on a 9-point scale). Well-established specific SCAR markers of ten Rpi genes—Rpi-R1, Rpi-R2/Rpi-blb3, Rpi-R3a, Rpi-R3b, Rpi-R8, Rpi-blb1/Rpi-sto1, Rpi-blb2, and Rpi-vnt1—were used to mine 117 accessions representing 20 species from seven Petota series. In particular, our evidence confirmed the diverse Rpi gene location in two American continents. The structural homologs of the Rpi-R2, Rpi-R3a, Rpi-R3b, and Rpi-R8 genes were found in the North American species other than S. demissum, the species that was the original source of these genes for early potato breeding, and in some cases, in the South American Tuberosa species. The Rpi-blb1/Rpi-sto1 orthologs from S. bulbocastanum and S. stoloniferum Schlechtd et Bché were restricted to genome B in the Mesoamerican series Bulbocastana, Pinnatisecta, and Longipedicellata. The structural homologs of the Rpi-vnt1 gene that were initially identified in the South American species S. venturii Hawkes and Hjert. were reported, for the first time, in the North American series of Petota species.

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Section: General Discussion and Conclusionmentioning

confidence: 80%

Section: General Discussion and Conclusionmentioning

confidence: 99%

Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Рогозина

Gurina

Chalaya

et al. 2023

Plants

View full text Add to dashboard Cite

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<i>In silico</i> search for and analysis of R gene variation in primitive cultivated potato species

Gurina,

Gancheva,

Alpatieva

et al. 2024

Vestn. VOGiS

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Pathogen recognition receptors encoded by R genes play a key role in plant protection. Nowadays, R genes are a basis for breeding many crops, including potato. Many potato R genes have been discovered and found suitable for breeding thanks to the studies of a wide variety of wild potato species. The use of primitive cultivated potato species (PCPS) as representatives of the primary gene pool can also be promising in this respect. PCPS are the closest to the early domesticated forms of potato; therefore, their investigation could help understand the evolution of R genes. The present study was aimed at identifying and analyzing R genes in PCPS listed in the open database of NCBI and Solomics DB. In total, the study involved 27 accessions belonging to three species: Solanum phureja Juz. & Bukasov, S. stenotomum Juz. & Bukasov and S. goniocalyx Juz. & Bukasov Materials for the analysis were the sequencing data for the said three species from the PRJNA394943 and PRJCA006011 projects. An in silico search was carried out for sequences homologous to 26 R genes identified in potato species differing in phylogenetic distance from PCPS, namely nightshade (S. americanum), North- (S. bulbocastanum, S. demissum) and South-American (S. venturii, S. berthaultii) wild potato species, as well as the cultivated potato species S. tuberosum and S. andigenum. Homologs of all investigated protein-coding sequences were discovered in PCPS with a relatively high degree of similarity (85–100 %). Homologs of the Rpi-R3b, Rpi-amr3 and Rpi-ber1 genes have been identified in PCPS for the first time. An analysis of polymorphism of nucleotide and amino acid sequences has been carried out for 15 R genes. The differences in frequencies of substitutions in PCPS have been demonstrated by analysis of R genes, the reference sequences of which have been identified in different species. For all the studied NBS-LRR genes, the proportion of substituted amino acids in the LRR domain exceeds this figure for the NBS domain. The potential prospects of using PCPS as sources of resistance to Verticillium wilt have been shown.

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Homologs of Late Blight Resistance Genes in Representatives of Tuber-Bearing Species of the Genus Solanum L.

Cited by 2 publications

References 40 publications

Diversity of Late Blight Resistance Genes in the VIR Potato Collection

Diversity of Late Blight Resistance Genes in the VIR Potato Collection

<i>In silico</i> search for and analysis of R gene variation in primitive cultivated potato species

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