Honey Flavonoids Inhibit
            <i>Candida albicans</i>
            Morphogenesis by Affecting DNA Behavior and Mitochondrial Function

Canonico, Barbara; Candiracci, Manila; Citterio, Barbara; Curci, Rosa; Squarzoni, Stefano; Mazzoni, Annalisa; Papa, Stefano; Piatti, Elena

doi:10.2217/fmb.14.17

Cited by 35 publications

(20 citation statements)

References 37 publications

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“…At 24 hr, all cells formed larger clumps and had transitioned mostly to hyphae (Figure ). Another related study examined the effects of a flavonoid rich honey extract on the C. albicans phenotypic switch using similar incubation time (6 and 18 hr) used herein (Canonico et al, ).…”

Section: Resultsmentioning

confidence: 99%

“…Yet, a critical lack of knowledge remains on the ability of phenolic synergists to prevent the C. albicans hyphae phenotypes, with notable exceptions (Canonico et al, ; Kazuko, Sato, & Azuma, ; Saito, Tamura, Imai, Ishigami, & Ochiai, ). However, the referenced research does not account for responsible components or confirm whether those agents acted synergistically by constructing isobolograms or calculating the combination index (CI) (Junio et al, ).…”

Section: Introductionmentioning

confidence: 99%

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Histone acetylation increases in response to ferulic, gallic, and sinapic acids acting synergistically in vitro to inhibit Candida albicans yeast‐to‐hyphae transition

Câmara

Shi

Pedersen

et al. 2018

Phytotherapy Research

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Novel treatments are needed to prevent candidiasis/candidemia infection due to the emergence of Candida species resistant to current antifungals. Considering the yeastto-hyphae switch is a critical factor to Candida albicans virulence, phenols common in plant sources have been reported to demonstrating their ability to prevent dimorphism. Therefore, phenols present in many agricultural waste stress (ferulic (FA) and gallic (GA) acid) were initially screened in isolation for their yeast-to-hyphae inhibitory properties at times 3, 6, and 24 hr. Both FA and GA inhibited 50% of hyphae formation inhibitory concentration (IC 50 ) but at a concentration of 8.0 ± 0.09 and 90.6 ± 1.05 mM, respectively, at 24 hr. However, the inhibitory effect of FA increased by 1.9-2.6 fold when combined with different GA concentrations. GA and FA values decreased even lower when sinapic acid (SA) was added as a third component. As evidenced by concave isobolograms and combination indexes less than 1, both GA:F A and GA:FA:SA combinations acted synergistically to inhibit 50% hyphae formation at 24 hr. Lastly, acetylation of histone H3 lysine 56 acetylation (H3K56) was higher in response to the triple phenolic cocktail (using the IC 50 24 hr inhibitory concentration level) comparable with the nontreated samples, indicating that the phenols inhibited hyphal growth in part by targeting H3K56 acetylation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histone acetylation increases in response to ferulic, gallic, and sinapic acids acting synergistically in vitro to inhibit Candida albicans yeast‐to‐hyphae transition

Câmara

Shi

Pedersen

et al. 2018

Phytotherapy Research

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“…It has been demonstrated that flavonoid part of honey decelerates the growth of fungi, affects the external morphology and membrane integrity, and inhibits some cellular processes that are involved in fungal growth and hyphal transition (Canonico et al . ).…”

Section: Resultsmentioning

confidence: 97%

Italian acacia honey exhibits lytic effects against the crayfish pathogensAphanomyces astaciandFusarium avenaceum

Sateriale

Scioscia

Colicchio

et al. 2018

Lett Appl Microbiol

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Significance and Impact of the Study: The continuous spread of aquatic fungal disease as the 'crayfish plague' and the 'burn spot disease' has severe ecological and commercial repercussions. Critical factor to prevent further spread is the availability of effective antifungals possibility derived from local natural resources to use in innovative strategies of control and eradication of these diseases. This study provides relevant information about the in vitro anti-oomycete and antifungal activity of Italian acacia honey aqueous extracts against two highly infectious and dangerous pathogenic species, Aphanomyces astaci and Fusarium avenaceum, that are responsible for important crayfish diseases. AbstractThis study purpose was to evaluate the in vitro inhibitory properties of Italian acacia honey extracts against pathogenic aquatic oomycete/fungal isolates that cause different diseases in crayfish, resulting in an elevated mortality rate. The antimycotic activity of acacia honey aqueous extracts was evaluated against the strain UEF88662 of Aphanomyces astaci (oomycete) and the strain SMM2 of Fusarium avenaceum (fungus). The extracts preparation was carried out with water by a cheap, not complex and organic solvent-free procedure, with low environmental impact and the higher possibility of large-scale reproducibility. The anti-oomycete and antifungal activities were quantitatively evaluated by growth, survival and sporulation microbiological assays. The extracts displayed a dose-dependent inhibitory efficacy on oomycete and fungal growth and survival, as well as on the production of oomycete and fungal spores. Supported by future in vivo studies, our results encourage the use of natural extracts like honey as innovative tools to counteract mycotic infections.Letters in Applied Microbiology 68, 64--72

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“…Manuka honey has also been shown to alter the structure of P. aeruginosa; scanning and transmission electron microscopy revealed changes in cell shape and cell lysis following incubation with honey 42 . A honey flavonoid extract was also found to alter membrane integrity and branching processes associated with virulence in C. albicans 43 .…”

Section: Accepted Manuscriptmentioning

confidence: 98%

Honey: A realistic antimicrobial for disorders of the skin

McLoone

Warnock

Fyfe

2016

Journal of Microbiology, Immunology and Infection

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Resistance of pathogenic microorganisms to antibiotics is a serious global health concern. In this review, research investigating the antimicrobial properties of honeys from around the world against skin relevant microbes is evaluated. A plethora of in vitro studies have revealed that honeys from all over the world have potent microbicidal activity against dermatologically important microbes. Moreover, in vitro studies have shown that honey can reduce microbial pathogenicity as well as reverse antimicrobial resistance. Studies investigating the antimicrobial properties of honey in vivo have been more controversial. It is evident that innovative research is required to exploit the antimicrobial properties of honey for clinical use and to determine the efficacy of honey in the treatment of a range of skin disorders with a microbiological etiology.

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Honey Flavonoids Inhibit Candida albicans Morphogenesis by Affecting DNA Behavior and Mitochondrial Function

Abstract: HFE improved mitochondrial functionality and reduced the vacuolization, modifying the branching process associated with virulence. It is hypothesized that HFE induces changes in cell cycle progress, membrane integrity, mitochondrial function and biogenesis.

Cited by 35 publications

References 37 publications

Histone acetylation increases in response to ferulic, gallic, and sinapic acids acting synergistically in vitro to inhibit Candida albicans yeast‐to‐hyphae transition

Histone acetylation increases in response to ferulic, gallic, and sinapic acids acting synergistically in vitro to inhibit Candida albicans yeast‐to‐hyphae transition

Italian acacia honey exhibits lytic effects against the crayfish pathogensAphanomyces astaciandFusarium avenaceum

Honey: A realistic antimicrobial for disorders of the skin

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