Honey improves spermatogenesis and hormone secretion in testicular ischaemia-reperfusion-induced injury in rats

Abstract

This study was conducted to survey the protective effect of pre-treatment with Persian honey during post-ischaemia reperfusion on ischaemia-reperfusion (IR)-induced testis injury. Animals were divided into four groups of IR, honey + ischaemia- reperfusion (HIR), vitamin C + ischaemia- reperfusion (VIR) and carbohydrates + ischaemia- reperfusion (CIR). The testes were examined for spermatogenesis index. Detection of single- and double-stranded DNA breaks at the early stages of apoptosis was performed. Total serum concentration of FSH, LH and testosterone was measured using ELISA. All data were expressed as mean ± SD in each group, and significance was set at p ≤ .05. Spermatogenesis index was significant in the HIR group (p < .001). Serum levels of FSH and LH were significantly higher in the CIR and HIR groups. Serum levels of testosterone were significantly higher in VIR and HIR groups. Apoptotic cells in IR and CIR groups increased significantly statistically (p < .001), while in HIR and VIR groups, the number of apoptotic cells decreased and the positive cells of TUNEL staining were detected in spermatocytes and spermatid. The present study indicates that honey decreases the cellular damage and apoptosis during testicular I/R injury, with significant protective effects on reproductive hormone production.

Troxerutin protect sperm, seminiferous epithelium and pituitary-gonadal axis from torsion-detorsion injury: An experimental study

“…Result of this study show that vitamin C only increase level of testosterone in the damage-induced torsion-detorsion testis. Natural and synthetic antioxidants such as melatonin, hydro alcoholic extract of citrus aurantifolia, coriandrum sativum L seed, quercetin, royal jelly and honey can protect testis from damage-induced by ischemia-reperfusion ( 2 , 39 - 46 ).…”

“…Testicular torsion is an acute surgical emergency that the delay in its treatment leads to ischemia and testicular injury ( 1 ). After interrupting the arterial blood flow, testis apoptosis will develope in a few hours ( 2 - 4 ). Testicular torsion is a serious problem in men, that if not treated can be lead to reduce fertility ( 3 ).…”

The effect of delivery type on neonatal blood indices in an Iranian population

“…For the first newborn, the hematological indexes (such as leukocytes, hematocrit, platelets, and hemoglobin) were higher than those for the second and third newborn. In women with high parity due to frequent childbirth and menstrual cycles, iron deficiency is greater than in nulliparous women [28][29][30] . Iron deficiency increases the risk of preterm childbirth and low weight in newborns.…”

Protective effects of royal jelly on testicular torsion induced ischaemia reperfusion injury in rats

“…The severity of the testicular torsional injury is associated with both the degree of torsion and the duration of ischaemia. Although the blood flow is completely halted at 720°, rotation of 360° or 720° was used in previous testis I/R rat models (Gholami et al., 2018; Ranade et al., 2011). In the present study, torsion has been applied in 720°.…”