Honey loaded silk fibroin 3D porous scaffold facilitates homeostatic full-thickness wound healing

Rajput, Monika; Mandal, Mousumi; Anura, Anji; Mukhopadhyay, Anurup; Subramanian, Bhuvaneshwaran; Paul, Ranjan Rashmi; Chatterjee, Jyotirmoy

doi:10.1016/j.mtla.2020.100703

Cited by 20 publications

(13 citation statements)

References 32 publications

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“…These two critical steps are essential for skin regeneration. Our results are consistent with those of previous studies that used SF dressing to improve wound healing [ 20 , 50 , 51 ]; however, previous studies did not focus on the influence of β-sheet SF. This study used 75% ethanol treatment to achieve an α-helix to β-sheet SF transition.…”

Section: Discussionsupporting

confidence: 92%

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

et al. 2021

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Therapeutic dressings to enhance burn wound repair and regeneration are required. Silk fibroin (SF), a natural protein, induces cell migration and serves as a biomaterial in various dressings. SF dressings usually contain α-helices and β-sheets. The former has been confirmed to improve cell proliferation and migration, but the wound healing effect and related mechanisms of β-sheet SF remain unclear. We investigated the effects of β-sheet SF in vivo and in vitro. Alcohol-treated α-helix SF transformed into the β-sheet form, which promoted granulation formation and re-epithelialization when applied as lyophilized SF dressing (LSFD) in a rat burn model. Our in vitro results showed that β-sheet SF increased human dermal fibroblast (HDF) migration and promoted the expression of extracellular matrix (ECM) proteins (fibronectin and type III collagen), matrix metalloproteinase-12, and the cell adhesion molecule, integrin β1, in rat granulation tissue and HDFs. This confirms the role of crosstalk between integrin β1 and ECM proteins in cell migration. In summary, we demonstrated that β-sheet SF facilitates tissue regeneration by modulating cell adhesion molecules in dermal fibroblasts. LSFD could find clinical application for burn wound regeneration. Moreover, β-sheet SF could be combined with anti-inflammatory materials, growth factors, or antibiotics to develop novel dressings.

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Section: Discussionsupporting

confidence: 92%

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

et al. 2021

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“…Therefore, recent research focuses on the development of different materials or matrices to convey honey, control its delivery, and act as absorbent secondary dressings [7,39,[93][94][95].…”

Section: Medical Grade Honey and Honey Ointments For Topical Applicationmentioning

confidence: 99%

Role of Honey in Advanced Wound Care

et al. 2021

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Honey is a natural product rich in several phenolic compounds, enzymes, and sugars with antioxidant, anticarcinogenic, anti-inflammatory, and antimicrobial potential. Indeed, the development of honey-based adhesives for wound care and other biomedical applications are topics being widely investigated over the years. Some of the advantages of the use of honey for wound-healing solutions are the acceleration of dermal repair and epithelialization, angiogenesis promotion, immune response promotion and the reduction in healing-related infections with pathogenic microorganisms. This paper reviews the main role of honey on the development of wound-healing-based applications, the main compounds responsible for the healing capacity, how the honey origin can influence the healing properties, also highlighting promising results in in vitro and in vivo trials. The challenges in the use of honey for wound healing are also covered and discussed. The delivery methodology (direct application, incorporated in fibrous membranes and hydrogels) is also presented and discussed.

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“…However, the degradation rate also increased in the fibrous mats with higher honey loading, together with a reduction in compressive strength. These effects on the mechanical features could be explained by the enhanced pore size and porosity of the tridimensional structures upon the incorporation of increasing honey quantities [171]. Similarly, when incorporated as an additive during the electrospinning process of silk fibroin (SF) solutions, Manuka honey has been found to function as an effective hydrophilicity-enhancer, significantly increasing the water retention capacity of the control (pristine silk fibroin scaffolds) at both the honey concentrations tested (1% and 5%), up to a maximum swelling ratio of almost 400% of the initial dry weight [172].…”

Section: Functional Aspects Of Honey-loaded Scaffolds For Wound Healingmentioning

confidence: 99%

“…The capacity of a wound dressing to swell and uptake a considerable volume when immersed in an extracellular fluid-resembling medium is indicative of its ability to absorb wound exudate and to maintain a curative moist environment on the affected skin area This sample had an optimal microstructure, which allowed a flux of nutritious substances to the wound site, with enhanced fibroblast proliferation and migration. In particular, a full skin regeneration occurred both at the epidermal and dermal level, with the prevalence of collagen I over collagen III, angiogenesis, and formation of skin appendages such as hair follicles and sebaceous glands after 2 weeks of treatment [171]. Similarly, dual crosslinked alginate hydrogels presented a disarranged crystalline structure for high honey concentrations, while honey incorporation up to 4% ensured a morphology favourable for cellular adhesion, a significantly better bactericidal activity compared to the other honey dilutions, and ultimately skin re-epithelialisation with characteristics indicative of healthy uninjured skin and minimal scar thickness [170].…”

Section: Functional Aspects Of Honey-loaded Scaffolds For Wound Healingmentioning

confidence: 99%

The Rediscovery of Honey for Skin Repair: Recent Advances in Mechanisms for Honey-Mediated Wound Healing and Scaffolded Application Techniques

2021

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Honey is a honey-bee product obtained mainly by the enzymatic processing of nectar from a variety of plants, which leads to the wide range of colours and flavours available on the market. These organoleptic and nutritional features are influenced by the chemical composition, which in turn depends on the botanical origin. Bioactive compounds account for honey beneficial activity in medical applications, which explains the extensive use of honey in ethno-pharmacology since antiquity, from cough remedies to dermatological treatments. Wound healing is one of the main therapeutic uses of honey, and various design options in pharmaceutical technology such as smart delivery systems and advanced dressings are currently being developed to potentiate honey’s valuable properties for better performance and improved final outcome. In this review, we will focus on the latest research that discloses crucial factors in determining what properties are most beneficial when considering honey as a medicinal product. We will present the most recent updates on the possible mechanisms responsible for the exceptional effects of this ageless therapeutical remedy on skin repair. Furthermore, the state-of-the-art in application techniques (incorporation into scaffolds as an alternative to direct administration) used to enhance honey-mediated wound-healing properties are explored.

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Honey loaded silk fibroin 3D porous scaffold facilitates homeostatic full-thickness wound healing

Cited by 20 publications

References 32 publications

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

Cutaneous Regeneration Mechanism of β-Sheet Silk Fibroin in a Rat Burn Wound Healing Model

Role of Honey in Advanced Wound Care

The Rediscovery of Honey for Skin Repair: Recent Advances in Mechanisms for Honey-Mediated Wound Healing and Scaffolded Application Techniques

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