Honeybee chitosan-melanin complex: isolation and investigation of antimicrobial activity

Manko, Nazar; Gromyko, Oleksandr; Tistechok, Stepan; Lutsyk, M.; Stoika, Rostyslav

doi:10.15407/ubj92.06.143

Cited by 5 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The index lower than control (0-hour time) was considered as candidacidal effect, while higher than control (0-hour time) but less than in control after 4 h of incubation was classified as growth inhibition, and higher than in control after 4 h of incubation indicated a stimulation of growth [35].…”

Section: Antimicrobial Methodsmentioning

confidence: 99%

Каркасні Арилсульфонаміди Та Їх Антимікробні Властивості

Palchykov¹,

Діль

Манько

et al. 2022

J. of Chem. and Tech.

View full text Add to dashboard Cite

Арилсульфонаміди, що містять (аза)норборнан, 3-азабіцикло[3.2.1]окт-6-ен та схожі фрагменти, були відібрані та протестовані на антимікробну активність до п’яти ключових ESKAPE патогенних бактерій, однієї грампозитивної бактерії, стійкої до метициліну Staphylococcus aureus (ATCC 43300), чотирьох грамнегативних бактерій, Escherichia coli (ATCC 25922), Klebsiella pneumonia (ATCC 700603), Acinetobacter baumannii (ATCC 19606) і Pseudomonas aeruginosa (ATCC 27853) і протигрибкову активність відносно двох патогенних штамів Candida albicans (ATCC 90028) та Cryptococcus neoformans var. Grubii (H99; ATCC 208821). Одна сполука з 4-нітрофенілсульфонамідним фрагментом продемонструвала високу активність щодо метицилін-резистентного Staphylococcus aureus (ATCC 43300). Сполука VP-4606 має низьку цитотоксичність щодо псевдонормальних клітин ліній HaCaT, Balb/c 3T3 та мітоген-активованих лімфоцитів, виділених із здорової дорослої периферичної крові здорового донора до 2,5 мМ.

show abstract

Section: Antimicrobial Methodsmentioning

confidence: 99%

Каркасні Арилсульфонаміди Та Їх Антимікробні Властивості

Palchykov¹,

Діль

Манько

et al. 2022

J. of Chem. and Tech.

View full text Add to dashboard Cite

show abstract

“…Thereafter 10 μl of MTT solution (5 mg/ml) was introduced and incubation was continued for 1 h. Cells were harvested by centrifugation 5 min at 1500 g, supernatant was discarded, small sediment was suspended in 1 ml of DMSO. After the incubation for 1 h at 37°C the OD of liquid was measured at 580 nm using spectrophotometer ULAB 102 UV (Ukraine) [29].…”

Section: Methodsmentioning

confidence: 99%

2-Amino-4,6,7,8-tetrahydrothiopyrano[3,2-b]pyran-3-carbonitrile 5,5-dioxide VP-4535 as an antimicrobial agent selective toward methicillin‐resistant Staphylococcus aureus

Palchykov¹

2022

Ukr. Biochem. J.

View full text Add to dashboard Cite

The antifungal activity was studied towards pathogenic fungal strains Candida albicans (ATCC 90028) and Cryptococcus neoformans var. Grubii (ATCC 208821). Compound VP-4535 bearing 5-methylindolin-2-one motif possessed the highest antibacterial activity and excellent selectivity toward methicillin-resistant Staphylococcus aureus but was inactive against non-resistant Staphylococcus aureus strain. The compound in therapeutic concentration was safe to human red blood cells, human lymphocytes, HaCaT,

show abstract

“…The index lower than control (0 hour) was considered as candidacidal effect, while higher than control (0 hour) but less than in control after 4 h of incubation was classified as growth inhibition, and higher than in control after 4 h of incubation indicated a stimulation of growth [25]. DMSO in such low concertation did not show inhibition effect on the growth of candida strains.…”

Section: сFu (Colony Forming Units) Assay On C Albicans Strainsmentioning

confidence: 97%

Synthesis of the novel cage amides and imides and evaluation of their antibacterial and antifungal activity

Palchykov¹,

Gaponov²,

Manko³

et al. 2022

Ukr.Biochem.J.

View full text Add to dashboard Cite

Cage amides and imides bearing bicyclo[2.2.1]- and bicyclo[2.2.2]-subunits were synthesized and evaluated both for antimicrobial activity toward five key ESKAPE pathogenic bacteria: one Gram‐positive bacteria methicillin‐resistant Staphylococcus aureus (ATCC 43300), four Gram‐negative bacteria Escherichia coli (ATCC 25922), Klebsiella pneumoniae (ATCC 700603), Acinetobacter baumannii (ATCC 19606) and Pseudomonas aeruginosa (ATCC 27853) and for antifungal activity towards pathogenic fungal strains Candida albicans (ATCC 90028) and Cryptococcus neoformans var. Grubii (H99; ATCC 208821). Compound VP-4539 with bicyclo[2.2.2]octene motif demonstrated the highest cytotoxic activity towards C. neoformans, while human keratinocytes of HaCaT line, murine fibroblasts of Balb/c 3T3 line and mitogen-activated lymphocytes of peripheral human blood were found to be tolerant to its action. VP-4539 compound did not intercalate into salmon sperm DNA indicating that its cytotoxicity is not related to intercalation into nucleic acid. Keywords: antifungal, antimicrobial, bicyclo[2.2.1]heptane, bicyclo[2.2.2]octene, cytotoxicity, DNA intercalation, human keratinocytes, lymphocytes, сage compounds

show abstract

Honeybee chitosan-melanin complex: isolation and investigation of antimicrobial activity

Cited by 5 publications

References 23 publications

Каркасні Арилсульфонаміди Та Їх Антимікробні Властивості

Каркасні Арилсульфонаміди Та Їх Антимікробні Властивості

2-Amino-4,6,7,8-tetrahydrothiopyrano[3,2-b]pyran-3-carbonitrile 5,5-dioxide VP-4535 as an antimicrobial agent selective toward methicillin‐resistant Staphylococcus aureus

Synthesis of the novel cage amides and imides and evaluation of their antibacterial and antifungal activity

Contact Info

Product

Resources

About