Honeysuckle extract relieves ovalbumin-induced allergic rhinitis by inhibiting AR-induced inflammation and autoimmunity

Abstract

Honeysuckle has antiviral, antioxidative and anti-inflammatory properties. Allergic rhinitis (AR) is induced by immunoglobulin E (IgE)-mediated inflammatory reaction. Our study investigates whether honeysuckle extract (HE) has therapeutic effect on AR. An AR model of mice was established by ovalbumin (OVA). Hematoxylin–Eosin staining was used to assess nasal mucosa damage. Enzyme-linked immunosorbent assay (ELISA) was performed to determine serum histamine, IgE and interleukin (IL)-2, IL-4, IL-17 and interferon-γ (IFN-γ) from nasal lavage fluid. Western blot was carried out to analyze the protein level from nasal mucosa tissue. We found that HE not only decreased nasal rubbing and sneezing in AR mice, but also reduced AR-induced damage to nasal mucosa. Moreover, HE lowered the levels of serum IgE and histamine and inhibited IL-4 and IL-17 levels from AR mice but raised IL-2 and IFN-γ levels in AR-induced nasal lavage fluid. Our results also showed that HE elevated the protein levels of forkhead box P3 (Foxp3) and T-box transcription factor (T-bet) in AR-induced nasal mucosa tissue, whereas it inhibited signal transducer and activator of transcription (STAT) 3 and GATA binding protein 3 (GATA-3) protein levels. By regulating AR-induced inflammatory reaction and autoimmune response, HE also relieved OVA-induced AR. Thus, HE could be used as a potential drug to treat AR.

Chlorogenic acid ameliorated allergic rhinitis-related symptoms in mice by regulating Th17 cells

“…However, this therapy was not effective and even caused further problems such as acute lung pathology [ 10 ]. Recent findings show that both Th17 cells and pTreg cells whose precursor cells are also CD4 T play vital roles in autoimmunity response that can be induced by AR [ 11 ]. Th17 cells can cause autoimmunity and inflammation, however which can be suppressed by Th17 cells to thus attain immune homeostasis [ 12 ].…”

The application of gargle containing honeysuckle and semen oroxyli to reduce the pain and complications after uvulopalatopharyngoplasty

“…Due to the excellent anti-inflammatory effect and low toxicity, we included honeysuckle and semen oroxyli in our self-made gargle with the aim to relive the pain and complications after UPPP. Actually, we did find that using the gargle containing honeysuckle and semen oroxyli could relieve both resting and Frontiers in Pharmacology frontiersin.org Total length of stay (day, median (IQR)) 8 (Yan et al, 2014;Lin et al, 2019;Yin et al, 2019) 8 (Yan et al, 2014;Lin et al, 2019;Yin et al, 2019) 0.740…”

“…Postoperative length of stay (day, median (IQR)) 5 (Ryu et al, 2010;Browaldh et al, 2013;Sommer et al, 2016) 5 (Ryu et al, 2010;Browaldh et al, 2013;Sommer et al, 2016;Lin et al, 2019) 0.816 BMI, body mass index; OSA, obstructive sleep apnea; ASA, american society of anesthesiologists; NYHA, new york heart association; IQR, interquartile range. swallowing throat pain after UPPP.…”

Integrative Analysis of Systems Pharmacology and Proteomics to Identify Key Targets and Pathways of Tuomin-Zhiti-Decoction for Allergic Rhinitis

“…During recent decades, Chinese herbal medicine, one of the traditional Chinese medicine that serves as a complementary and alternative medicine, has been used as popular strategies for treating AR and aroused a remarkable and growing interest [5][6][7] . Tuomin-Zhiti-Decoction (TZD), which was optimized based on Chinese herbal formula discovered by well-known professor Qi Wang, is a novel formula of Chinese medicine that has been used to effectively treat AR during recent years.…”