Hordeum Vulgare : A Suitable Host for Mass Production of Arbuscular Mycorrhizal Fungi From Natural Soil

Abstract

Abstract. Four plant species viz. Hordeum vulgare, Triticum aestivum, Phaseolus vulgaris and Phaseolus mungo were examined for mass production of consortium of AM fungi present in the rhizosphere soil. Rhizosphere soil were collected from teak dominated forest and was used as a source of AM inoculum. Surface sterilized seeds of all the four selected plant species were raised in a mixture of sterilized soil and sand in 3:1 ratio. Mass production of AM fungi was observed in terms of (%) AM colonization, AM spore population, and effectiveness of AM consortia was recorded in terms of height and dry weight of inoculated and uninoculated plants. Out of four test species H. vulgare responded as the most suitable host. After 60 days, H. vulgare showed the highest colonization (92%) and 74 spores per 25 g soil. It was observed that plants having higher AM colonization showed higher AM spore production showing a positive correlation, however, it was not found in T. aestivum. Study indicates that H. vulgare is suitable for early colonization and mass production of AM fungi. After 90 days of pot culture, pot soil from all host were analyzed for AM flora. In all, eleven AM species were identified from the consortium. Maximum numbers of AM species (8 species) were found associated with H. vulgare. Three species of AM fungi i.e. Glomus aggregatum, G. fasciculatum and Sclerocystis pakistanica were found to dominate in the soil of H. vulgare.

Screening and selection of efficient host and sugarcane bagasse as substrate for mass multiplication ofFunneliformis mosseae

“…Use of maize and lemon grass for AM fungal multiplication is also suggested by Tahat et al (2008) and Kaushish et al (2011), and the suitability of these species could be due to the production of a wide variety of water-soluble and volatile organic compounds that may serve as attractants, nutrient sources and even as genetic regulatory signals for AM fungi for better colonization (Koide & Schreiner 1992). The host plants used in this study are members of Poaceae, and effective AM symbiosis in these hosts is attributed to secondary roots and their high density, which favours root colonization and spore production (Chaurasia & Khare 2005). Likewise, efficacy of various grasses of the Bangalore (India) region was screened for their suitability for mass production of Glomus fasciculatum, and guinea grass [Megathyrsus maximus (Jacq.)…”

Promiscuous arbuscular mycorrhizal symbiosis of yam (Dioscorea spp.), a key staple crop in West Africa

“…This further indicates that AMF may play an important role in yam nutrition and tuber production and explain the good production performance of yam immediately following savanna clearance, when AMF abundance is high as compared to subsequent years (Tchabi et al 2008). Plant growth promotion by AMF is a wellestablished phenomenon across crops and climatic zones (e.g., Chaurasia and Hare 2005;Caglar and Akgun 2006;Smith and Read 2008). Further studies on yam growth promotion by AMF should now focus on the specificity and functionality of the symbiotic interaction and the influence of inocula levels on AMF-yam interactions under controlled and field conditions.…”

The Indigenous endomycorrhizal fungi at salak (Salacca zalacca) plantations in Bali, Indonesia and their colonization of the roots

“…Soil samples were taken from the rhizosphere around the base of the bark. Samples were taken 30-40 cm around the base of the stem and at a depth of 10-15 cm from the ground surface to collect about 2 kg of soil (Chaurasia and Khare 2005;Nurhandayani et al 2013). Root samples (10 cm) were taken from the same points were put into polyethylene bags and stored at 4 o C in the laboratory.…”