Horizontal Gel Electrophoresis for Enhanced Detection of Protein-RNA Complexes

Dowdle, Megan E.; Imboden, Susanne Blaser; Park, Sookhee; Ryder, Seán; Sheets, Michael

doi:10.3791/56031-v

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…After turning on the power, gels were run under 120 V at 4°C in the dark. Next, images were captured by using an imager [25]; the surface morphology of 3WJ‐apt‐miR was examined using Atomic Force Microscopy (AFM).…”

Section: Methodsmentioning

confidence: 99%

Preparation, characterisation, and in vitro cancer‐suppression function of RNA nanoparticles carrying miR‐301b‐3p Inhibitor

Zhao

Yang

Liu

et al. 2023

IET Nanobiotechnology

View full text Add to dashboard Cite

Background Multidrug resistance is the biggest barrier on the way to chemotherapy for lung adenocarcinoma (LUAD). For some LUAD patients with cisplatin (DDP) resistance and poor prognoses, the authors put forward RNA nanoparticles (NPs) carrying miR‐301b‐3p Inhibitor. Methods The NPs were composed of miR‐301b‐3p, A549 aptamer (A549apt), and Cyanine 5 in a bottom‐up manner with a 3‐way‐junction (3WJ) structure. Diameter, assembly process, and morphology of NPs were observed by Dynamic Light Scattering, Native‐Polyacrylamide Gel Electrophoresis, and Atomic Force Microscopy. Cell internalisation, toxicity, proliferation, migration, invasion, and apoptosis were assayed by confocal laser scanning microscope, CCK8, colony formation assay, Transwell, western blot, and flow cytometry. Results 3WJ‐apt‐miR was evenly distributed, with diameter of 19.61 ± 0.49 nm and triangular branching structures. The accurate delivery of this NP in vivo was ensured by A549 aptamer featuring specific targeting, with smaller side effects than traditional chemotherapy. Such nanomaterials were effectively internalized by cancer cells, with normal cell activity intact. Cancer cell proliferation, invasion, and migration were suppressed, and DDP sensitivity was enhanced, causing DNA damage and facilitating apoptosis of DDP‐resistant cells. Conclusion Based on RNA self‐assembling, the authors researched the effect of miRNA on DDP sensitivity in LUAD regarding gene regulation. 3WJ‐apt‐miR paves the way for clinical tumour therapy.

show abstract

Section: Methodsmentioning

confidence: 99%