Horizontal Honey-Bee Larvae Rearing Plates Can Increase the Deformation Rate of Newly Emerged Adult Honey Bees

Kim, Juyeong; Chon, Kyongmi; Kim, Bo-Seon; Oh, Jeongseog; Yoon, Chang-Young; Park, Hong-Hyun; Choi, Yong‐Soo

doi:10.3390/insects12070603

Cited by 6 publications

(3 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The higher mortality in the May session is associated with a protocol that was under development. Vertical capping plates are thought to significantly reduce the deformation rate of newly emerged adults but, in our experience, increase mortality and do not allow us to monitor mortality on a daily basis (Kim et al, 2021). During the June and August sessions, we applied the modification proposed by Schmel et al to transfer the larvae at day 8, as has been done in other publications on larval rearing (Mortensen & Ellis, 2023; Mortensen et al, 2019; Schmehl et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Experimental Ecotoxicology Procedures Interfere with Honey Bee Life History

Desclos le Peley,

Grateau,

Moreau‐Vauzelle

et al. 2024

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

Apis mellifera was used as a model species for ecotoxicological testing. In the present study, we tested the effects of acetone (0.1% in feed), a solvent commonly used to dissolve pesticides, on bees exposed at different developmental stages (larval and/or adult). Moreover, we explored the potential effect of in vitro larval rearing, a commonly used technique for accurately monitoring worker exposure at the larval stage, by combining acetone exposure and treatment conditions (in vitro larval rearing vs. in vivo larval rearing). We then analyzed the life‐history traits of the experimental bees using radio frequency identification technology over three sessions (May, June, and August) to assess the potential seasonal dependence of the solvent effects. Our results highlight the substantial influence of in vitro larval rearing on the life cycle of bees, with a 47.7% decrease in life span, a decrease of 0.9 days in the age at first exit, an increase of 57.3% in the loss rate at first exit, and a decrease of 40.6% in foraging tenure. We did not observe any effect of exposure to acetone at the larval stage on the capacities of bees reared in vitro. Conversely, acetone exposure at the adult stage reduced the bee life span by 21.8% to 60%, decreased the age at first exit by 1.12 to 4.34 days, and reduced the foraging tenure by 30% to 37.7%. Interestingly, we found a significant effect of season on acetone exposure, suggesting that interference with the life‐history traits of honey bees is dependent on season. These findings suggest improved integration of long‐term monitoring for assessing sublethal responses in bees following exposure to chemicals during both the larval and adult stages. Environ Toxicol Chem 2024;00:1–12. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.

show abstract

Section: Discussionmentioning

confidence: 99%

Experimental Ecotoxicology Procedures Interfere with Honey Bee Life History

Desclos le Peley,

Grateau,

Moreau‐Vauzelle

et al. 2024

Enviro Toxic and Chemistry

View full text Add to dashboard Cite

show abstract

“…However, surviving bees were evaluated and weighed on D21 (test termination). Adult deformation symptoms were classified based on the method described by Kim et al 47 and the following as: surviving normal (SN), deformed with no wings (DNW), deformed with short wings (DSW), deformed with tangled wings (DTW) and deformed antennae (DA). If wing deformation was observed on only one wing, it was recorded by adding asymmetric (A) to the end of the symptom name.…”

Section: Mortality Emergence Rate and Deformation Rate Of Honey Bee L...mentioning

confidence: 99%

Assessment of acute and chronic toxicity of cyantraniliprole and sulfoxaflor on honey bee (Apis mellifera) larvae

Kim

Chon

Kim

et al. 2022

Pest Management Science

Self Cite

View full text Add to dashboard Cite

BACKGROUND: Recently, cyantraniliprole (CYA) and sulfoxaflor (SUL) have been considered as alternatives to neonicotinoid insecticides. In this study, we evaluated the acute and chronic toxicities of CYA and SUL on honey bee (Apis mellifera L.) larvae reared in vitro. RESULTS:In the acute toxicity test, the following test doses were used to determine the median lethal dose (LD 50 ): CYA 0.007, 0.014, 0.028, 0.056 and 0.112 ∼g larva −1 ; SUL 2.5, 5, 10, 20 and 40 ∼g larva −1 . In the chronic toxicity test, the following test doses were used to determine the LD 50 : CYA 0.00512, 0.0128, 0.032, 0.08 and 0.2 ∼g larva −1 ; SUL 0.0625, 0.125, 0.25, 0.5 and 1.0 ∼g larva −1 . The acute LD 50 values of CYA and SUL were 0.047 and 11.404 ∼g larva −1 , respectively. Larvae acutely exposed to SUL had significantly lower body weight than controls, but those exposed to CYA showed no difference. The no observed adverse effect level (NOAEL) and LD 50 values of the chronic toxicity tests for each insecticide were 0.00512 and 0.064 ∼g larva −1 for CYA, and 0.0625 ∼g larva −1 and 0.212 ∼g larva −1 for SUL, respectively. Larvae chronically exposed to SUL emerged as bees with deformed wings, reaching adult deformation rates of over 50%; however, CYA had no effect on adult deformation. CONCLUSION: Exposure to CYA increased larval mortality but did not cause any adult deformation, whereas SUL exposure increased pupal mortality and caused wing deformation in newly emerged bees. Our study may be useful for the assessment of pesticide toxicity by providing valuable findings on the effects of these insecticides on honey bee larvae.

show abstract

“…Rearing honeybee (Apis mellifera) larvae in vitro is an important method for studying bee larvae diseases or the toxicity of pesticides on bees. Kim et al [9] evaluated the emergence and deformation rates of honeybee (Apis mellifera ligustica Spinola) larvae reared in horizontal and vertical positions, finding that a vertical rearing method was the better approach. Compared to the horizontal rearing plates, the vertical rearing plates resulted in a twofold decrease in adult deformation rates and larger adults (11.6 mm vs. 10.8 mm).…”

mentioning

confidence: 99%