“…Cell migration was studied by the IncuCyte scratch assay modified by An et al 22 The results showed that NDF, DDF, and DWF treated with film extract for 24 h elucidated a completely healed scratch after 48 h, while under other conditions (DMEM with 0% FBS and DMEM with 2% FBS), they exhibit no wound closure at the same time, as shown in Figure 4 A. At 48 h, percentages of relative wound density for NDF, DDF, and DWF treated with film extract were 99.73 ± 5.01, 98.01 ± 2.90, and 93.47 ± 2.86%, respectively, which reached approximately 100% of relative wound density, whereas they were lower for NDF, DDF, and DWF cultured with DMEM with 0% FBS (32.65 ± 3.52, 37.01 ± 1.75, and 38.63 ± 2.36%) and DMEM with 2% FBS (68.15 ± 4.91, 59.37 ± 6.85, and 48.27 ± 2.71%) ( Figure 4 B).…”