1988
DOI: 10.1002/bies.950080606
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Hormonal and heat‐stress regulation of protein synthesis in the aleurone layers of barley seeds

Abstract: Barley aleurone cells have long served as a model system for studying the regulation of gene expression in plants. In this review we survey what is known about hormone‐regulated gene expression in aleurone cells. We also describe the effects of heat stress on gene expression in this system, and speculate how the aleurone cell prioritizes its response between hormone‐induced and environment‐induced programs of gene expression.

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Cited by 10 publications
(5 citation statements)
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“…With the exception of hybridizations using the Volvox f3-tubulin clone (where stringency was significantly reduced), the membrane was prehybridized, hybridized, and washed according to the procedures of Church and Gilbert (8). Prehybridization and hybridization with reduced stringency (for the ,B-tubulin clone) were done in a solution containing 50% formamide, 5 x SSC (0.75 M NaCl, 0.075 M Na citrate, pH 7.0) and 100 ,ug/mL sheared salmon sperm DNA at 42°C. Under reduced stringency conditions, the hybridized membranes were washed (10 min each) three times in 2 x SSC, 0.1% SDS at room temperature; once in 2 x SSC, 0.1% SDS at 60°C, and once in 0.2 x SSC, 0.…”
Section: Rna Analysismentioning
confidence: 99%
“…With the exception of hybridizations using the Volvox f3-tubulin clone (where stringency was significantly reduced), the membrane was prehybridized, hybridized, and washed according to the procedures of Church and Gilbert (8). Prehybridization and hybridization with reduced stringency (for the ,B-tubulin clone) were done in a solution containing 50% formamide, 5 x SSC (0.75 M NaCl, 0.075 M Na citrate, pH 7.0) and 100 ,ug/mL sheared salmon sperm DNA at 42°C. Under reduced stringency conditions, the hybridized membranes were washed (10 min each) three times in 2 x SSC, 0.1% SDS at room temperature; once in 2 x SSC, 0.1% SDS at 60°C, and once in 0.2 x SSC, 0.…”
Section: Rna Analysismentioning
confidence: 99%
“…Isolated embryos synthesize gibberellins, whereas embryoless grains or isolated aleurone layers generally fail to synthesize hydrolytic enzymes. Addition of gibberellins or gibberellin-synthesizing embryos to half-grains or to aleurone layers induces a-amylase synthesis in a dose-dependent manner (2,3,13).Synthesis of gibberellins by isolated embryos, however, does not necessarily reflect the situation in whole grains. Radley (17) shows that an isolated scutellum synthesizes gibberellins, whereas a scutellum attached to the grain does not; synthesis of gibberellins in isolated embryos can be completely inhibited by low concentrations of sugars.…”
mentioning
confidence: 99%
“…Isolated embryos synthesize gibberellins, whereas embryoless grains or isolated aleurone layers generally fail to synthesize hydrolytic enzymes. Addition of gibberellins or gibberellin-synthesizing embryos to half-grains or to aleurone layers induces a-amylase synthesis in a dose-dependent manner (2,3,13).…”
mentioning
confidence: 99%
“…They proposed that ER plays an important role in stabilizing a-amylase mRNA in aleurone cells. They argued that, since a-amylase is a secreted protein that is synthesized on membrane-bound ribosomes, the stability of mRNA for this protein is a consequence of its binding to the endomembrane system (3,4).…”
mentioning
confidence: 99%