Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA

Nilsson, Ove; Moritz, Thomas; Imbault, Nadine; Sandberg, Göran; Olsson, Olof

doi:10.1104/pp.102.2.363

Cited by 94 publications

(54 citation statements)

References 33 publications

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“…Various degrees of rolC expression were shown in relation to the extent of the dwarf phenotype ( Figures 3A and B). This indicates that the rolC gene might play a key role in the phenotypic variation described above, which is consistent with previous reports (Nilsson et al 1993;Winefield et al 1999). The corolla tissues also exhibited rolC expression, and their extent of expression was negatively correlated with corolla length (Figures 3A and C).…”

Section: Phenotypic Features Of Dwarf Gentianssupporting

confidence: 82%

Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes

Mishiba

Nishihara

Abe

et al. 2006

Plant Biotechnology

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We successfully produced dwarf potted gentian using wild-type Agrobacterium rhizogenes A4 strain (ATCC43057) harboring an agropine type plasmid (pRiA4). A number of hairy roots were induced by direct inoculation with A. rhizogenes to the stem and leaf tissues in vitro. Adventitious shoots were regenerated from the hairy roots on Murashige and Skoog medium containing 10 mg l Ϫ1 N-phenyl-NЈ-(1,2,3-thiadiazol-5-yl) urea, 0.1 or 1.0 mg l Ϫ1 1-naphthaleneacetic acid, and 3 g l Ϫ1 gellan gum. Regenerated plants were then cultured in vitro for at least six months and tested for the absence of viable A. rhizogenes within their tissues. After receiving authorization from the Ministry of Agriculture, Forestry and Fisheries, Japan, the plants were acclimated and grown in a greenhouse and/or field. Opine synthesis and rolC gene expression were analyzed to demonstrate successful introduction and expression of the transferred DNA. In total, 122 lines of variant types of dwarf plants with blue, white, and pink flowers were obtained, which might be useful for molecular breeding of a series of dwarf potted gentian cultivars.

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Section: Phenotypic Features Of Dwarf Gentianssupporting

confidence: 82%

Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes

Mishiba

Nishihara

Abe

et al. 2006

Plant Biotechnology

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“…In most cases only minor changes in the content of free cytokinins were measured when different tissues of constitutively expressing tobacco or potato plants were analyzed with immunoassays or LC-M~" (Nilsson et aL, 1993b;SchmQIling et al, 1993a). This could be ultimately due to homeostatic mechanisms that could mask primary events and blur differences in constitutively expressing plants.…”

Section: Introductionmentioning

confidence: 99%

Chemically induced expression of the rolC‐encoded β‐glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosides in planta

et al. 1996

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SummaryThe rolC gene of Agrobacterium rhizogenes T-DNA plays an essential role in the establishment of hairy root disease and its overexpression in transgenic plants causes pleiotropic developmental alterations. This study investigated whether the biological activity of the rolC 13-glucosidase is due to an alteration of the cytokinin balance in planta.HPLC radiocounting assays of [3H]-Iabeled cytokinin glucosides fed exogenously to tobacco leaf disks, to rolC expressing Escherichia coil cells or cell-free extracts showed that cytokinin N3-and O-glucosides are the preferred substrate of the rolC protein. Hydrolysis of N7-and N9-glucosides was not detected at substrate concentrations close to physiological levels. Furthermore, these conjugates were also not active as cytokinins in biotests when fed to rolC-expressing tissues. For analysis of the rolC activity on endogenous cytokinin conjugates the gene was expressed under the transcriptional control of a modified tetracycline-inducible 35S promoter. This was done to avoid possible interference with secondary effects or plant homeostatic mechanisms which could mask primary in planta events when transgenes are expressed constitutively. No changes in the endogenous pool of different cytokinin glucosides, as determined by a newly developed electrospray tandem mass spectroscopy directly coupled to high performance liquid chromatography, were found following chemical induction of the rolC gene. Also the levels of free cytokinins remained unchanged after gene induction. Hybrid tobacco plants expressing the cytokinin-

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“…To date, gene constructs that contain the single rolC gene expressed from the cauliflower mosaic virus 35s promoter have been used to transform species such as tobacco (Nicotiana tabacum) (Schmiilling et al, 1988;Nilsson et al, 1993), potato (Solanum tuberosum) (Fladung, 1990), and Atropa belladonna (Kurioka et al, 1992). The growth and development of the transformants have been drastically altered.…”

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confidence: 99%

Expression of the Agrobacterium rhizogenes rolC Gene in a Deciduous Forest Tree Alters Growth and Development and Leads to Stem Fasciation

et al. 1996

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We have altered the growth and development of a deciduous forest tree by transforming hybrid aspen (Populus tremula x Populus tremuloides) with the Agrobacterium rhizogenes rolC gene expressed under the strong cauliflower mosaic virus 35s promoter. We demonstrate that the genetically manipulated perennial plants, after a period of dormancy, maintain the induced phenotypical changes during the second growing period. Furthermore, massspectrometrical quantifications of the free and conjugated forms of indole-3-acetic acid and cytokinins and severa1 gibberellins on one transgenic line correlate the induced developmental alterations such as stem fasciation to changes in plant hormone metabolism. We also show that the presence of the RolC protein increases the levels of the free cytokinins, but not by a process involving hydrolysis of the inactive cytokinin conjugates.Genetic engineering of annual plant species has proven to be a valuable technique both for commercial applications and to manipulate and to study the different aspects of plant biology. With the recent development of new transformation protocols, these techniques now can be extended to commercially and biologically interesting perennials such as fruit and forest trees to alter the quality and yield parameters and also to learn more about how growth and development are regulated in woody plants. However, although these techniques are available and the list of transgenic tree species is constantly growing (reviewed by Jouanin et al., 1993), there are still only a few reports available on the regeneration of phenotypically altered trees due to the controlled, ectopic expression of a heterologous gene (Weigel and Nilsson, 1995;Tuominen et al., 1995).We transformed hybrid aspen (Populus tremula X Populus tremuloides), a temperate-zone deciduous tree species, with a gene that has been demonstrated to drastically alter the

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Hormonal Characterization of Transgenic Tobacco Plants Expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA

Cited by 94 publications

References 33 publications

Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes

Production of dwarf potted gentian using wild-type Agrobacterium rhizogenes

Chemically induced expression of the rolC‐encoded β‐glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosides in planta

Expression of the Agrobacterium rhizogenes rolC Gene in a Deciduous Forest Tree Alters Growth and Development and Leads to Stem Fasciation

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