1996
DOI: 10.1002/(sici)1520-6327(1996)32:3/4<467::aid-arch18>3.3.co;2-5
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Hormonal control of expression of a pupal cuticular protein gene during metamorphosis in Galleria

Abstract: To trace the developmentally regulated expression of a gene coding for a pupal cuticle protein (GmPCP52), total RNA of carcasses was extracted at different times during metamorphosis and subjected to Northern blot analysis. Blots were probed with a dig-labelled antisense in vitro transcript of GmPCP52 cDNA.In untreated animals, GrnPCP52 mRNA is absent during the wandering stage in last instar larvae. The amount of mRNA rises after pupal ecdysis to a maximum at a pupal age of 18 h. At 42 h it again becomes unde… Show more

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Cited by 1 publication
(6 citation statements)
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“…This time course fits well to northern blot data (Krämer and Wolbert 1996), which have shown that the GmPCP52 transcript is still undetectable immediately before pupal ecdysis and can be found only in trace amounts immediately thereafter. Then the transcript gradually accumulates as long as transcription continues.…”
Section: Discussionsupporting
confidence: 87%
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“…This time course fits well to northern blot data (Krämer and Wolbert 1996), which have shown that the GmPCP52 transcript is still undetectable immediately before pupal ecdysis and can be found only in trace amounts immediately thereafter. Then the transcript gradually accumulates as long as transcription continues.…”
Section: Discussionsupporting
confidence: 87%
“…The developmental profile of in vitro translatable pupal cuticle protein mRNAs (Wolbert 1985;Wolbert and Schäfer 1991) closely follows that of poly(A) tail shortening of the GmPCP52 transcript. Therefore, we conclude, that most of what can be seen in the northern blots (Krämer and Wolbert 1996) is accumulation of translationally inactive material. Most studies in developmental regulation of gene expression in insect metamorphosis are based on northern blot or in situ hybridization.…”
Section: Discussionmentioning
confidence: 56%
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