Hormonal reactivity in tooth germsin vitro: Effects of parathyroid hormone and calcitonin

Gozariu, L; Barabás, Éva; Cristea, M; Abraham, A. D.

doi:10.1007/bf02064118

Cited by 4 publications

(3 citation statements)

References 3 publications

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“…Neither were there significant differences in the calcium concentration reductions in the medium between the treated explants and their controls. These findings are in contrast to those reported by Gozariu et al 5 and by Mallek et al 15, who used an in vitro system to study the direct effects of PTH and CT on tooth germ mineralization. However, their methods apparently disrupted the integrity of the enamel organ and are irrelevant to the study of calcium transport through the enamel organ.…”

Section: Methodscontrasting

confidence: 67%

The Effects of Parathyroid Hormone, Calcitonin, and Vitamin D Metabolites on Calcium Transport in the Secretory Rat Enamel Organ

1983

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Section: Methodscontrasting

confidence: 67%

The Effects of Parathyroid Hormone, Calcitonin, and Vitamin D Metabolites on Calcium Transport in the Secretory Rat Enamel Organ

1983

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“…The primary role of PTH and CT is to maintain serum cail cium and phosphorus concentrations in the extracellular fluid in the physiological range. Several investigators have bcused on the uptake and release of calcium in tooth germs (KIine, 1978: 1981-Gozariu et at, 1977Gozariu and Baiabas, 1978;MMallek et at, 1979) and the transport of calcium through the enamel organ of developing teeth (Bawden ec a, 1983(Bawden ec a, , 1985.…”

Section: Methodsmentioning

confidence: 98%

“…With regard to the uptake or release of calcium in tooth germs, Kline (1978Kline ( , 1981 reported that CTtreated tooth germs took up a higher amount of calcium than did the controls. Gozariu et al (1977) and Gozariu and Barabas (1978) showed that CT inhibited 45Ca-uptake by molar tooth germs. Calcium release from the tooth germs was stimulated by PTH, but CT blocked the PTH effect.…”

Section: Introductionmentioning

confidence: 96%

In vitro Effects of Calcitonin and/or Parathyroid Hormone on Odontogenesis of Mouse Embryonic Molars

et al. 1989

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Mandibular first molars of mouse embryos were cultivated for examination of the effects of calcitonin (CT) and/or parathyroid hormone (PTH) on the odontogenesis of the molars, and for determination of whether and how CT, which is a PTH antagonist, has an influence on the effect of PTH on odontogenesis. On the second day, the inner enamel epithelium in the control group had already differentiated into pre-ameloblasts. Typical odontoblasts had secreted a layer of predentin. On the fourth day of culture, the pre-ameloblasts achieved terminal differentiation into secretory ameloblasts, and enamel and dentin had already been deposited. PTH (1 unit/mL) inhibited the odontogenesis of the cultured molars during the designated culture periods (two and four days), while CT (0.5 unit/mL) stimulated odontogenesis. On the second day, the development of the molars in the CT + PTH group showed an intermediate stage between the control and PTH-treated explants, but on day 4 it corresponded to that of the controls. Moreover, when the molars exposed to PTH for two days were untreated and treated with CT for an additional two days, the former produced a small quantity of enamel matrix, while the latter formed a large amount of the matrix. These histological findings were also supported by a morphometric analysis of the enamel matrix in the cultured molars. The present results suggest that CT stimulates, but PTH suppresses, the odontogenesis of the mouse embryonic molars, and that CT is an antagonist to the inhibitory effect of PTH on odontogenesis.

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Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar toothIn vitro

Sakakura

1987

Calcif Tissue Int

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Mandibular first molars of 17-day-old mouse embryos were cultured in vitro to examine the histological effects of various concentrations of parathyroid hormone (PTH) on odontogenesis of the molars. PTH did not affect the cytodifferentiation of mesenchymal cells into preodontoblasts but inhibited that of preodontoblasts into odontoblasts. Consequently, the odontoblasts failed to undergo dentinogenesis. On the other hand, inner enamel epithelium achieved terminal cytodifferentiation into secretory ameloblasts and these cells partially formed enamel in spite of the absence of dentin. All treated molars showed the same histological disturbances and these effects were independent of PTH dose. The present study indicated that PTH had an influence on mesenchyme-derived cells, inhibiting both the differentiation of odontoblasts and the formation of predentin and dentin.

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Hormonal reactivity in tooth germsin vitro: Effects of parathyroid hormone and calcitonin

Cited by 4 publications

References 3 publications

The Effects of Parathyroid Hormone, Calcitonin, and Vitamin D Metabolites on Calcium Transport in the Secretory Rat Enamel Organ

The Effects of Parathyroid Hormone, Calcitonin, and Vitamin D Metabolites on Calcium Transport in the Secretory Rat Enamel Organ

In vitro Effects of Calcitonin and/or Parathyroid Hormone on Odontogenesis of Mouse Embryonic Molars

Effects of parathyroid hormone on odontogenesis of the mouse embryonic molar toothIn vitro

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