Hormonal regulation changes in shrimp infected with Enterocytozoon hepatopenaei: a transcriptomic analysis

Zhang, Leiting; Zhang, Sheng; Dou, Yabin; Shen, Hui; Qiao, Yi; Cao, Xiaohui; Fan, Xianping; Hu, Yadong; Qian, Jin

doi:10.1007/s10499-023-01299-x

Cited by 1 publication

(1 citation statement)

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“…Therefore, effective genetic management is crucial to safeguard the health and adaptability of aquaculture populations. When genes associated with immunity [ 84 , 85 ], reproduction [ 86 , 87 ], environmental stress response [ 68 , 88 , 89 , 90 ], behavior [ 91 ], and other functions experience a loss of diversity, the population’s capacity to adapt is compromised, thereby heightening the risk of encountering various ecological pressures. The analysis of functional markers corresponds to key functional genes associated with resistance traits against White Spot Syndrome Virus (WSSV) and Acute Hepatopancreatic Necrosis Disease (AHPND) found in the transcriptome sequencing dataset.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Insights and the Development of Microsatellite Markers to Assess Genetic Diversity in the Broodstock Management of Litopenaeus stylirostris

Yang,

Chu,

Chen

et al. 2024

Animals

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The Pacific blue shrimp (Litopenaeus stylirostris) is a premium product in the international seafood market. However, intensified farming has increased disease incidence and reduced genetic diversity. In this study, we developed a transcriptome database for L. stylirostris and mined microsatellite markers to analyze their genetic diversity. Using the Illumina HiSeq 4000 platform, we identified 53,263 unigenes from muscle, hepatopancreas, the intestine, and lymphoid tissues. Microsatellite analysis identified 36,415 markers from 18,657 unigenes, predominantly dinucleotide repeats. Functional annotation highlighted key disease resistance pathways and enriched categories. The screening and PCR testing of 42 transcriptome-based and 58 literature-based markers identified 40 with successful amplification. The genotyping of 200 broodstock samples revealed that Na, Ho, He, PIC, and FIS values were 3, 0.54 ± 0.05, 0.43 ± 0.09, 0.41 ± 0.22, and 0.17 ± 0.27, respectively, indicating moderate genetic variability and significant inbreeding. Four universal microsatellite markers (CL1472.Contig13, CL517.Contig2, Unigene5692, and Unigene7147) were identified for precise diversity analysis in Pacific blue, Pacific white (Litopenaeus vannamei), and black tiger shrimps (Penaeus monodon). The transcriptome database supports the development of markers and functional gene analysis for selective breeding programs. Our findings underscore the need for an appropriate genetic management system to mitigate inbreeding depression, reduce disease susceptibility, and preserve genetic diversity in farmed shrimp populations.

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